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A novel lignin in poplar trees with a reduced caffeic acid/5-hydroxyferulic acid O-methyltransferase activity

(1995) PLANT JOURNAL. 8(6). p.855-864
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Abstract
Lignin is a polymeric constituent of the cell wall that needs to be removed during the paper making process. Bispecific caffeic acid/5-hydroxyferulic acid O-methyltransferase (COMT) catalyses the O-methylation of caffeic acid and 5-hydroxyferulic acid to ferulic acid and sinapic acid, respectively. These compounds are intermediates in the biosynthesis of the lignin precursors. Therefore, COMTs are potential target enzymes for reducing the amount, or modifying the composition, of lignin in plants. Different antisense and sense constructs have been expressed of a gene encoding a COMT from poplar (Populus trichocarpa X II: deltoides) in a I! tremula X P. alba clone under the control of the cauliflower mosaic virus 35S promoter. From all analysed transformants, four lines transformed with an antisense construct had a reduced COMT activity. Two showed a 50% reduction of COMT activity, which altered only slightly the monomeric composition. In the two other transform ants, the COMT activity was reduced by 95%. In the latter case, the syringyl/guaiacyl ratio (S/G) was reduced by sixfold (due to a decrease of S and an increase of G), as analysed by thioacidolysis. A new component of lignin, the 5-hydroxy-guaiacyl residue, was detected among the thioacidolysis products. Moreover, in contrast to the white/yellow colour of wild-type wood, the xylem of the transgenic lines with a 95% reduction of COMT activity was pale rose. A similar phenotype was observed in brown-midrib mutants of maize and sorghum, known for their altered lignification. Although the lignin composition was consistently modified, the lignin content of the transgenic poplars was similar to that of the controls.
Keywords
BROWN-MIDRIB MUTANT, CINNAMYL-ALCOHOL-DEHYDROGENASE, PURIFICATION, EXPRESSION, SEQUENCE, TOBACCO, GENES, WOOD, LIGNIFICATION, GUAIACYL

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Citation

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Chicago
Van Doorsselaere, Jan, Marie Baucher, Emmanuelle Chognot, Brigitte Chabbert, Marie-Thérèse Tollier, Michel Petit-Conil, Jean-Charles Leplé, et al. 1995. “A Novel Lignin in Poplar Trees with a Reduced Caffeic Acid/5-hydroxyferulic Acid O-methyltransferase Activity.” Plant Journal 8 (6): 855–864.
APA
Van Doorsselaere, Jan, Baucher, M., Chognot, E., Chabbert, B., Tollier, M.-T., Petit-Conil, M., Leplé, J.-C., et al. (1995). A novel lignin in poplar trees with a reduced caffeic acid/5-hydroxyferulic acid O-methyltransferase activity. PLANT JOURNAL, 8(6), 855–864.
Vancouver
1.
Van Doorsselaere J, Baucher M, Chognot E, Chabbert B, Tollier M-T, Petit-Conil M, et al. A novel lignin in poplar trees with a reduced caffeic acid/5-hydroxyferulic acid O-methyltransferase activity. PLANT JOURNAL. 1995;8(6):855–64.
MLA
Van Doorsselaere, Jan, Marie Baucher, Emmanuelle Chognot, et al. “A Novel Lignin in Poplar Trees with a Reduced Caffeic Acid/5-hydroxyferulic Acid O-methyltransferase Activity.” PLANT JOURNAL 8.6 (1995): 855–864. Print.
@article{186294,
  abstract     = {Lignin is a polymeric constituent of the cell wall that needs to be removed during the paper making process. Bispecific caffeic acid/5-hydroxyferulic acid O-methyltransferase (COMT) catalyses the O-methylation of caffeic acid and 5-hydroxyferulic acid to ferulic acid and sinapic acid, respectively. These compounds are intermediates in the biosynthesis of the lignin precursors. Therefore, COMTs are potential target enzymes for reducing the amount, or modifying the composition, of lignin in plants. Different antisense and sense constructs have been expressed of a gene encoding a COMT from poplar (Populus trichocarpa X II: deltoides) in a I! tremula X P. alba clone under the control of the cauliflower mosaic virus 35S promoter. From all analysed transformants, four lines transformed with an antisense construct had a reduced COMT activity. Two showed a 50\% reduction of COMT activity, which altered only slightly the monomeric composition. In the two other transform ants, the COMT activity was reduced by 95\%. In the latter case, the syringyl/guaiacyl ratio (S/G) was reduced by sixfold (due to a decrease of S and an increase of G), as analysed by thioacidolysis. A new component of lignin, the 5-hydroxy-guaiacyl residue, was detected among the thioacidolysis products. Moreover, in contrast to the white/yellow colour of wild-type wood, the xylem of the transgenic lines with a 95\% reduction of COMT activity was pale rose. A similar phenotype was observed in brown-midrib mutants of maize and sorghum, known for their altered lignification. Although the lignin composition was consistently modified, the lignin content of the transgenic poplars was similar to that of the controls.},
  author       = {Van Doorsselaere, Jan and Baucher, Marie and Chognot, Emmanuelle and Chabbert, Brigitte and Tollier, Marie-Th{\'e}r{\`e}se and Petit-Conil, Michel and Lepl{\'e}, Jean-Charles and Pilate, Gilles and Cornu, Daniel and Monties, Bernard and Van Montagu, Marc and Inz{\'e}, Dirk and Boerjan, Wout and Jouanin, Lise},
  issn         = {0960-7412},
  journal      = {PLANT JOURNAL},
  keyword      = {BROWN-MIDRIB MUTANT,CINNAMYL-ALCOHOL-DEHYDROGENASE,PURIFICATION,EXPRESSION,SEQUENCE,TOBACCO,GENES,WOOD,LIGNIFICATION,GUAIACYL},
  language     = {eng},
  number       = {6},
  pages        = {855--864},
  title        = {A novel lignin in poplar trees with a reduced caffeic acid/5-hydroxyferulic acid O-methyltransferase activity},
  url          = {http://dx.doi.org/10.1046/j.1365-313X.1995.8060855.x},
  volume       = {8},
  year         = {1995},
}

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