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The antibacterial activity of biogenic silver and its mode of action

Liesje Sintubin UGent, Bart De Gusseme UGent, Paul Van Der Meeren UGent, Benny Pycke UGent, Willy Verstraete UGent and Nico Boon UGent (2011) APPLIED MICROBIOLOGY AND BIOTECHNOLOGY. 91(1). p.153-162
abstract
In a previous study, biogenic silver nanoparticles were produced by Lactobacillus fermentum which served as a matrix preventing aggregation. In this study the antibacterial activity of this biogenic silver was compared to ionic silver and chemically produced nanosilver. The minimal inhibitory concentration (MIC) was tested on Gram-positive and Gram-negative bacteria and was comparable for biogenic silver and ionic silver ranging from 12.5 to 50 mg/L. In contrast, chemically produced nanosilver had a much higher MIC of at least 500 mg/L, due to aggregation upon application. The minimal bactericidal concentration (MBC) in drinking water varied from 0.1 to 0.5 mg/L for biogenic silver and ionic silver, but for chemically produced nanosilver concentrations, up to 12.5 mg/L was needed. The presence of salts and organic matter decreased the antimicrobial activity of all types of silver resulting in a higher MBC and a slower inactivation of the bacteria. The mode of action of biogenic silver was mainly attributed to the release of silver ions due to the high concentration of free silver ions measured and the resemblance in performance between biogenic silver and ionic silver. Radical formation by biogenic silver and direct contact were found to contribute little to the antibacterial activity. In conclusion, biogenic nanosilver exhibited equal antimicrobial activity compared to ionic silver and can be a valuable alternative for chemically produced nanosilver.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
BIOLOGICAL APPROACH, ESCHERICHIA-COLI, MEDIATED SYNTHESIS, DRINKING-WATER, BY-PRODUCTS, NANOPARTICLES, IONS, NITRATE, DISINFECTION, ZEOLITE, Biocide, Nanoparticles, Green chemistry, Biological synthesis, Drinking water, Disinfectant
journal title
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
Appl. Microbiol. Biotechnol.
volume
91
issue
1
pages
153 - 162
Web of Science type
Article
Web of Science id
000291600600013
JCR category
BIOTECHNOLOGY & APPLIED MICROBIOLOGY
JCR impact factor
3.425 (2011)
JCR rank
39/157 (2011)
JCR quartile
1 (2011)
ISSN
0175-7598
DOI
10.1007/s00253-011-3225-3
project
Biotechnology for a sustainable economy (Bio-Economy)
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
1857878
handle
http://hdl.handle.net/1854/LU-1857878
date created
2011-07-13 09:32:04
date last changed
2013-02-27 12:59:20
@article{1857878,
  abstract     = {In a previous study, biogenic silver nanoparticles were produced by Lactobacillus fermentum which served as a matrix preventing aggregation. In this study the antibacterial activity of this biogenic silver was compared to ionic silver and chemically produced nanosilver. The minimal inhibitory concentration (MIC) was tested on Gram-positive and Gram-negative bacteria and was comparable for biogenic silver and ionic silver ranging from 12.5 to 50 mg/L. In contrast, chemically produced nanosilver had a much higher MIC of at least 500 mg/L, due to aggregation upon application. The minimal bactericidal concentration (MBC) in drinking water varied from 0.1 to 0.5 mg/L for biogenic silver and ionic silver, but for chemically produced nanosilver concentrations, up to 12.5 mg/L was needed. The presence of salts and organic matter decreased the antimicrobial activity of all types of silver resulting in a higher MBC and a slower inactivation of the bacteria. The mode of action of biogenic silver was mainly attributed to the release of silver ions due to the high concentration of free silver ions measured and the resemblance in performance between biogenic silver and ionic silver. Radical formation by biogenic silver and direct contact were found to contribute little to the antibacterial activity. In conclusion, biogenic nanosilver exhibited equal antimicrobial activity compared to ionic silver and can be a valuable alternative for chemically produced nanosilver.},
  author       = {Sintubin, Liesje and De Gusseme, Bart and Van Der Meeren, Paul and Pycke, Benny and Verstraete, Willy and Boon, Nico},
  issn         = {0175-7598},
  journal      = {APPLIED MICROBIOLOGY AND BIOTECHNOLOGY},
  keyword      = {BIOLOGICAL APPROACH,ESCHERICHIA-COLI,MEDIATED SYNTHESIS,DRINKING-WATER,BY-PRODUCTS,NANOPARTICLES,IONS,NITRATE,DISINFECTION,ZEOLITE,Biocide,Nanoparticles,Green chemistry,Biological synthesis,Drinking water,Disinfectant},
  language     = {eng},
  number       = {1},
  pages        = {153--162},
  title        = {The antibacterial activity of biogenic silver and its mode of action},
  url          = {http://dx.doi.org/10.1007/s00253-011-3225-3},
  volume       = {91},
  year         = {2011},
}

Chicago
Sintubin, Liesje, Bart De Gusseme, Paul Van Der Meeren, Benny Pycke, Willy Verstraete, and Nico Boon. 2011. “The Antibacterial Activity of Biogenic Silver and Its Mode of Action.” Applied Microbiology and Biotechnology 91 (1): 153–162.
APA
Sintubin, L., De Gusseme, B., Van Der Meeren, P., Pycke, B., Verstraete, W., & Boon, N. (2011). The antibacterial activity of biogenic silver and its mode of action. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 91(1), 153–162.
Vancouver
1.
Sintubin L, De Gusseme B, Van Der Meeren P, Pycke B, Verstraete W, Boon N. The antibacterial activity of biogenic silver and its mode of action. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY. 2011;91(1):153–62.
MLA
Sintubin, Liesje, Bart De Gusseme, Paul Van Der Meeren, et al. “The Antibacterial Activity of Biogenic Silver and Its Mode of Action.” APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 91.1 (2011): 153–162. Print.