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Post-transcriptional β-1,3-glucanase gene silencing involves increased transcript turnover that is translation-independent

(1997) PLANT JOURNAL. 12(4). p.885-893
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Abstract
In tobacco line T17, basic beta-1,3-glucanases are silenced when a beta-1,3-glucanase (gn1) transgene of Nicotiana plumbaginifolia is present in a homozygous condition. Cosuppression of endogenous and transgenic basic beta-1,3-glucanases occurs via a post transcriptional process. This study shows that silencing correlates with a reduced stability of the transcripts encoded by gn1 and the co-suppressed endogenous glucanase genes. The observed decreases in mRNA stability at least partially explain the reductions in mRNA level. Furthermore, the results show that the enhanced, silencing-related turnover of gn1 mRNA does not require continued protein synthesis. The implications of these findings for the mechanism of gene silencing are discussed.
Keywords
COSUPPRESSION, ACCUMULATION, LEVEL, SUPPRESSION, PLANTS, RNA TURNOVER, DNA-METHYLATION, TRANSGENE EXPRESSION, VIRUS-RESISTANCE, TOBACCO

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Chicago
Jacobs, John JMR, Katia Litière, Victor van Dijk, Gerben J van Eldik, Marc Van Montagu, and Marc Cornelissen. 1997. “Post-transcriptional Β-1,3-glucanase Gene Silencing Involves Increased Transcript Turnover That Is Translation-independent.” Plant Journal 12 (4): 885–893.
APA
Jacobs, J. J., Litière, K., van Dijk, V., van Eldik, G. J., Van Montagu, M., & Cornelissen, M. (1997). Post-transcriptional β-1,3-glucanase gene silencing involves increased transcript turnover that is translation-independent. PLANT JOURNAL, 12(4), 885–893.
Vancouver
1.
Jacobs JJ, Litière K, van Dijk V, van Eldik GJ, Van Montagu M, Cornelissen M. Post-transcriptional β-1,3-glucanase gene silencing involves increased transcript turnover that is translation-independent. PLANT JOURNAL. 1997;12(4):885–93.
MLA
Jacobs, John JMR, Katia Litière, Victor van Dijk, et al. “Post-transcriptional Β-1,3-glucanase Gene Silencing Involves Increased Transcript Turnover That Is Translation-independent.” PLANT JOURNAL 12.4 (1997): 885–893. Print.
@article{185785,
  abstract     = {In tobacco line T17, basic beta-1,3-glucanases are silenced when a beta-1,3-glucanase (gn1) transgene of Nicotiana plumbaginifolia is present in a homozygous condition. Cosuppression of endogenous and transgenic basic beta-1,3-glucanases occurs via a post transcriptional process. This study shows that silencing correlates with a reduced stability of the transcripts encoded by gn1 and the co-suppressed endogenous glucanase genes. The observed decreases in mRNA stability at least partially explain the reductions in mRNA level. Furthermore, the results show that the enhanced, silencing-related turnover of gn1 mRNA does not require continued protein synthesis. The implications of these findings for the mechanism of gene silencing are discussed.},
  author       = {Jacobs, John JMR and Litière, Katia and van Dijk, Victor and van Eldik, Gerben J and Van Montagu, Marc and Cornelissen, Marc},
  issn         = {0960-7412},
  journal      = {PLANT JOURNAL},
  keywords     = {COSUPPRESSION,ACCUMULATION,LEVEL,SUPPRESSION,PLANTS,RNA TURNOVER,DNA-METHYLATION,TRANSGENE EXPRESSION,VIRUS-RESISTANCE,TOBACCO},
  language     = {eng},
  number       = {4},
  pages        = {885--893},
  title        = {Post-transcriptional β-1,3-glucanase gene silencing involves increased transcript turnover that is translation-independent},
  url          = {http://dx.doi.org/10.1046/j.1365-313X.1997.12040885.x},
  volume       = {12},
  year         = {1997},
}

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