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Cigarette smoking alters intestinal barrier function and Peyer's Patch composition

STEPHANIE VERSCHUERE (UGent) , Ken Bracke (UGent) , Tine Demoor (UGent) , Maud Plantinga (UGent) , Bart Lambrecht (UGent) , Guy Brusselle (UGent) and Claude Cuvelier (UGent)
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Abstract
Smokers have a two-fold increased risk to develop Crohn’s disease (CD). However, little is known about the mechanisms through which smoking affects CD pathogenesis. Interestingly, the Peyer’s patches in the terminal ileum are the sites where the first CD lesions develop. To investigate whether smoke exposure causes alterations in Peyer’s patches, we studied C57BL/6 mice after exposure to air or cigarette smoke for 24 weeks. First, barrier function of the follicle-associated epithelium overlying Peyer’s patches was evaluated. We demonstrate that chronic smoke exposure is associated with increased apoptosis in the follicle-associated epithelium. Furthermore, immune cell numbers and differentiation along with chemokine expression were determined in the ileal Peyer’s patches. We observed significant increases in total dendritic cells (DC), CD4+ T-cells (including regulatory T-cells) and CD8+ T-cells after smoke exposure compared with air-exposed animals. The CD11b+ DC subset almost doubled. Interestingly, these changes were accompanied by an up-regulated mRNA expression of the chemokines CCL9 and CCL20, which are known to attract CD11b+ DC towards the subepithelial dome of Peyer’s patches. Our results demonstrate that cigarette smoke exposure induces apoptosis in follicle-associated epithelium and is associated with immune cell accumulation in Peyer’s patches, changes which can predispose to the development of CD.
Keywords
mouse model, epithelial barrier, Peyer's patches, Cigarette smoke

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Chicago
VERSCHUERE, STEPHANIE, Ken Bracke, Tine Demoor, Maud Plantinga, Bart Lambrecht, Guy Brusselle, and Claude Cuvelier. 2011. “Cigarette Smoking Alters Intestinal Barrier Function and Peyer’s Patch Composition.” In Mucosal Immunology, 15th International Congress, Abstracts.
APA
VERSCHUERE, S., Bracke, K., Demoor, T., Plantinga, M., Lambrecht, B., Brusselle, G., & Cuvelier, C. (2011). Cigarette smoking alters intestinal barrier function and Peyer’s Patch composition. Mucosal Immunology, 15th International congress, Abstracts. Presented at the 15th International congress of Mucosal Immunology (ICMI 2011).
Vancouver
1.
VERSCHUERE S, Bracke K, Demoor T, Plantinga M, Lambrecht B, Brusselle G, et al. Cigarette smoking alters intestinal barrier function and Peyer’s Patch composition. Mucosal Immunology, 15th International congress, Abstracts. 2011.
MLA
VERSCHUERE, STEPHANIE, Ken Bracke, Tine Demoor, et al. “Cigarette Smoking Alters Intestinal Barrier Function and Peyer’s Patch Composition.” Mucosal Immunology, 15th International Congress, Abstracts. 2011. Print.
@inproceedings{1857788,
  abstract     = {Smokers have a two-fold increased risk to develop Crohn{\textquoteright}s disease (CD). However, little is known about the mechanisms through which smoking affects CD pathogenesis. Interestingly, the Peyer{\textquoteright}s patches in the terminal ileum are the sites where the first CD lesions develop. To investigate whether smoke exposure causes alterations in Peyer{\textquoteright}s patches, we studied C57BL/6 mice after exposure to air or cigarette smoke for 24 weeks. First, barrier function of the follicle-associated epithelium overlying Peyer{\textquoteright}s patches was evaluated. We demonstrate that chronic smoke exposure is associated with increased apoptosis in the follicle-associated epithelium. Furthermore, immune cell numbers and differentiation along with chemokine expression were determined in the ileal Peyer{\textquoteright}s patches. We observed significant  increases in total dendritic cells (DC), CD4+ T-cells (including regulatory T-cells) and CD8+ T-cells after smoke exposure compared with air-exposed animals. The CD11b+ DC subset almost doubled. Interestingly, these changes were accompanied by an up-regulated mRNA expression of the chemokines CCL9 and CCL20, which are known to attract CD11b+ DC towards the subepithelial dome of Peyer{\textquoteright}s patches. Our results demonstrate that cigarette smoke exposure induces apoptosis in follicle-associated epithelium and is associated with immune cell accumulation in Peyer{\textquoteright}s patches, changes which can predispose to the development of CD.},
  author       = {VERSCHUERE, STEPHANIE and Bracke, Ken and Demoor, Tine and Plantinga, Maud and Lambrecht, Bart and Brusselle, Guy and Cuvelier, Claude},
  booktitle    = {Mucosal Immunology, 15th International congress, Abstracts},
  keyword      = {mouse model,epithelial barrier,Peyer's patches,Cigarette smoke},
  language     = {eng},
  location     = {Paris, France},
  title        = {Cigarette smoking alters intestinal barrier function and Peyer's Patch composition},
  year         = {2011},
}