Ghent University Academic Bibliography

Advanced

A new method for non-invasive estimation of human muscle fiber type composition

Audrey Baguet UGent, Inge Everaert UGent, Peter Hespel, Mirko Petrovic UGent, Eric Achten UGent and Wim Derave UGent (2011) PLOS ONE. 6(7).
abstract
Background: It has been established that excellence in sports with short and long exercise duration requires a high proportion of fast-twitch (FT) or type-II fibers and slow-twitch (ST) or type-I fibers, respectively. Until today, the muscle biopsy method is still accepted as gold standard to measure muscle fiber type composition. Because of its invasive nature and high sampling variance, it would be useful to develop a non-invasive alternative. Methodology: Eighty-three control subjects, 15 talented young track-and-field athletes, 51 elite athletes and 14 ex-athletes volunteered to participate in the current study. The carnosine content of all 163 subjects was measured in the gastrocnemius muscle by proton magnetic resonance spectroscopy ((1)H-MRS). Muscle biopsies for fiber typing were taken from 12 untrained males. Principal Findings: A significant positive correlation was found between muscle carnosine, measured by (1)H-MRS, and percentage area occupied by type II fibers. Explosive athletes had similar to 30% higher carnosine levels compared to a reference population, whereas it was similar to 20% lower than normal in typical endurance athletes. Similar results were found in young talents and ex-athletes. When active elite runners were ranked according to their best running distance, a negative sigmoidal curve was found between logarithm of running distance and muscle carnosine. Conclusions: Muscle carnosine content shows a good reflection of the disciplines of elite track-and-field athletes and is able to distinguish between individual track running distances. The differences between endurance and sprint muscle types is also observed in young talents and former athletes, suggesting this characteristic is genetically determined and can be applied in early talent identification. This quick method provides a valid alternative for the muscle biopsy method. In addition, this technique may also contribute to the diagnosis and monitoring of many conditions and diseases that are characterized by an altered muscle fiber type composition.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
IN-VIVO, CROSS-SECTIONS, RELAXATION-TIME, CARNOSINE CONCENTRATIONS, INTENSITY EXERCISE PERFORMANCE, HUMAN SKELETAL-MUSCLE, BETA-ALANINE SUPPLEMENTATION, HUMAN VASTUS LATERALIS, SPRINTERS, CAPACITY
journal title
PLOS ONE
PLoS One
volume
6
issue
7
article_number
e21956
pages
6 pages
Web of Science type
Article
Web of Science id
000292655400031
JCR category
BIOLOGY
JCR impact factor
4.092 (2011)
JCR rank
12/84 (2011)
JCR quartile
1 (2011)
ISSN
1932-6203
DOI
10.1371/journal.pone.0021956
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
1856511
handle
http://hdl.handle.net/1854/LU-1856511
date created
2011-07-08 18:26:16
date last changed
2015-06-17 09:48:59
@article{1856511,
  abstract     = {Background: It has been established that excellence in sports with short and long exercise duration requires a high proportion of fast-twitch (FT) or type-II fibers and slow-twitch (ST) or type-I fibers, respectively. Until today, the muscle biopsy method is still accepted as gold standard to measure muscle fiber type composition. Because of its invasive nature and high sampling variance, it would be useful to develop a non-invasive alternative. Methodology: Eighty-three control subjects, 15 talented young track-and-field athletes, 51 elite athletes and 14 ex-athletes volunteered to participate in the current study. The carnosine content of all 163 subjects was measured in the gastrocnemius muscle by proton magnetic resonance spectroscopy ((1)H-MRS). Muscle biopsies for fiber typing were taken from 12 untrained males. Principal Findings: A significant positive correlation was found between muscle carnosine, measured by (1)H-MRS, and percentage area occupied by type II fibers. Explosive athletes had similar to 30\% higher carnosine levels compared to a reference population, whereas it was similar to 20\% lower than normal in typical endurance athletes. Similar results were found in young talents and ex-athletes. When active elite runners were ranked according to their best running distance, a negative sigmoidal curve was found between logarithm of running distance and muscle carnosine. Conclusions: Muscle carnosine content shows a good reflection of the disciplines of elite track-and-field athletes and is able to distinguish between individual track running distances. The differences between endurance and sprint muscle types is also observed in young talents and former athletes, suggesting this characteristic is genetically determined and can be applied in early talent identification. This quick method provides a valid alternative for the muscle biopsy method. In addition, this technique may also contribute to the diagnosis and monitoring of many conditions and diseases that are characterized by an altered muscle fiber type composition.},
  articleno    = {e21956},
  author       = {Baguet, Audrey and Everaert, Inge and Hespel, Peter and Petrovic, Mirko and Achten, Eric and Derave, Wim},
  issn         = {1932-6203},
  journal      = {PLOS ONE},
  keyword      = {IN-VIVO,CROSS-SECTIONS,RELAXATION-TIME,CARNOSINE CONCENTRATIONS,INTENSITY EXERCISE PERFORMANCE,HUMAN SKELETAL-MUSCLE,BETA-ALANINE SUPPLEMENTATION,HUMAN VASTUS LATERALIS,SPRINTERS,CAPACITY},
  language     = {eng},
  number       = {7},
  pages        = {6},
  title        = {A new method for non-invasive estimation of human muscle fiber type composition},
  url          = {http://dx.doi.org/10.1371/journal.pone.0021956},
  volume       = {6},
  year         = {2011},
}

Chicago
Baguet, Audrey, Inge Everaert, Peter Hespel, Mirko Petrovic, Eric Achten, and Wim Derave. 2011. “A New Method for Non-invasive Estimation of Human Muscle Fiber Type Composition.” Plos One 6 (7).
APA
Baguet, A., Everaert, I., Hespel, P., Petrovic, M., Achten, E., & Derave, W. (2011). A new method for non-invasive estimation of human muscle fiber type composition. PLOS ONE, 6(7).
Vancouver
1.
Baguet A, Everaert I, Hespel P, Petrovic M, Achten E, Derave W. A new method for non-invasive estimation of human muscle fiber type composition. PLOS ONE. 2011;6(7).
MLA
Baguet, Audrey, Inge Everaert, Peter Hespel, et al. “A New Method for Non-invasive Estimation of Human Muscle Fiber Type Composition.” PLOS ONE 6.7 (2011): n. pag. Print.