Advanced search
1 file | 1.83 MB Add to list

Inositol trisphosphate-induced Ca2+ signaling modulates auxin transport and PIN polarity

(2011) DEVELOPMENTAL CELL. 20(6). p.855-866
Author
Organization
Abstract
The phytohormone auxin is an important determinant of plant development. Directional auxin flow within tissues depends on polar localization of PIN auxin transporters. To explore regulation of PIN-mediated auxin transport, we screened for suppressors of PIN1 overexpression (supo) and identified an inositol polyphosphate 1-phosphatase mutant (supo1), with elevated inositol trisphosphate (InsP(3)) and cytosolic Ca2+ levels. Pharmacological and genetic increases in InsP(3) or Ca2+ levels also suppressed the PIN1 gain-of-function phenotypes and caused defects in basal PIN localization, auxin transport and auxin-mediated development. In contrast, the reductions in InsP(3) levels and Ca2+ signaling antagonized the effects of the supo1 mutation and disrupted preferentially apical PIN localization. InsP(3) and Ca2+ are evolutionarily conserved second messengers involved in various cellular functions, particularly stress responses. Our findings implicate them as modifiers of cell polarity and polar auxin transport, and highlight a potential integration point through which Ca2+ signaling-related stimuli could influence auxin-mediated development.
Keywords
ENDOPLASMIC-RETICULUM, ARABIDOPSIS-THALIANA, POLYPHOSPHATE 1-PHOSPHATASE, PLANT DEVELOPMENT, CALCIUM PUMPS, EFFLUX CARRIER, NEGATIVE REGULATOR, ABSCISIC-ACID, ADVENTITIOUS ROOT-FORMATION, PROTEIN

Downloads

  • (...).pdf
    • full text
    • |
    • UGent only
    • |
    • PDF
    • |
    • 1.83 MB

Citation

Please use this url to cite or link to this publication:

MLA
Zhang, Jing, Steffen Vanneste, Philip B Brewer, et al. “Inositol Trisphosphate-induced Ca2+ Signaling Modulates Auxin Transport and PIN Polarity.” DEVELOPMENTAL CELL 20.6 (2011): 855–866. Print.
APA
Zhang, Jing, Vanneste, S., Brewer, P. B., Michniewicz, M., Grones, P., Kleine-Vehn, J., Löfke, C., et al. (2011). Inositol trisphosphate-induced Ca2+ signaling modulates auxin transport and PIN polarity. DEVELOPMENTAL CELL, 20(6), 855–866.
Chicago author-date
Zhang, Jing, Steffen Vanneste, Philip B Brewer, Marta Michniewicz, Peter Grones, Jürgen Kleine-Vehn, Christian Löfke, et al. 2011. “Inositol Trisphosphate-induced Ca2+ Signaling Modulates Auxin Transport and PIN Polarity.” Developmental Cell 20 (6): 855–866.
Chicago author-date (all authors)
Zhang, Jing, Steffen Vanneste, Philip B Brewer, Marta Michniewicz, Peter Grones, Jürgen Kleine-Vehn, Christian Löfke, Thomas Teichmann, Agnieszka Bielach, Bernard Cannoot, Klára Hoyerová, Xu Chen, Hong-Wei Xue, Eva Benkova, Eva Zazímalová, and Jiri Friml. 2011. “Inositol Trisphosphate-induced Ca2+ Signaling Modulates Auxin Transport and PIN Polarity.” Developmental Cell 20 (6): 855–866.
Vancouver
1.
Zhang J, Vanneste S, Brewer PB, Michniewicz M, Grones P, Kleine-Vehn J, et al. Inositol trisphosphate-induced Ca2+ signaling modulates auxin transport and PIN polarity. DEVELOPMENTAL CELL. 2011;20(6):855–66.
IEEE
[1]
J. Zhang et al., “Inositol trisphosphate-induced Ca2+ signaling modulates auxin transport and PIN polarity,” DEVELOPMENTAL CELL, vol. 20, no. 6, pp. 855–866, 2011.
@article{1854197,
  abstract     = {The phytohormone auxin is an important determinant of plant development. Directional auxin flow within tissues depends on polar localization of PIN auxin transporters. To explore regulation of PIN-mediated auxin transport, we screened for suppressors of PIN1 overexpression (supo) and identified an inositol polyphosphate 1-phosphatase mutant (supo1), with elevated inositol trisphosphate (InsP(3)) and cytosolic Ca2+ levels. Pharmacological and genetic increases in InsP(3) or Ca2+ levels also suppressed the PIN1 gain-of-function phenotypes and caused defects in basal PIN localization, auxin transport and auxin-mediated development. In contrast, the reductions in InsP(3) levels and Ca2+ signaling antagonized the effects of the supo1 mutation and disrupted preferentially apical PIN localization. InsP(3) and Ca2+ are evolutionarily conserved second messengers involved in various cellular functions, particularly stress responses. Our findings implicate them as modifiers of cell polarity and polar auxin transport, and highlight a potential integration point through which Ca2+ signaling-related stimuli could influence auxin-mediated development.},
  author       = {Zhang, Jing and Vanneste, Steffen and Brewer, Philip B and Michniewicz, Marta and Grones, Peter and Kleine-Vehn, Jürgen and Löfke, Christian and Teichmann, Thomas and Bielach, Agnieszka and Cannoot, Bernard and Hoyerová, Klára and Chen, Xu and Xue, Hong-Wei and Benkova, Eva and Zazímalová, Eva and Friml, Jiri},
  issn         = {1534-5807},
  journal      = {DEVELOPMENTAL CELL},
  keywords     = {ENDOPLASMIC-RETICULUM,ARABIDOPSIS-THALIANA,POLYPHOSPHATE 1-PHOSPHATASE,PLANT DEVELOPMENT,CALCIUM PUMPS,EFFLUX CARRIER,NEGATIVE REGULATOR,ABSCISIC-ACID,ADVENTITIOUS ROOT-FORMATION,PROTEIN},
  language     = {eng},
  number       = {6},
  pages        = {855--866},
  title        = {Inositol trisphosphate-induced Ca2+ signaling modulates auxin transport and PIN polarity},
  url          = {http://dx.doi.org/10.1016/j.devcel.2011.05.013},
  volume       = {20},
  year         = {2011},
}

Altmetric
View in Altmetric
Web of Science
Times cited: