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Influence of C4-dicarboxylic acid transporters on succinate production

Joeri Beauprez UGent, Maria R Foulquié-Moreno, Jo Maertens UGent, Ellen Van Horen UGent, Katelijne Bekers, Gino Baart UGent, Raymond M Cunin, Daniel Charlier, Joseph J Heijnen and Wim Soetaert UGent (2011) GREEN CHEMISTRY. 13(8). p.2179-2186
abstract
Current climate issues and the ongoing depletion of oil reserves have led to increased attention for biobased production processes. Not only has the production of bio-energy gained interest, but also the production of biochemicals. Succinate is one of those biochemicals. In the presented work, the dicarboxylic acid transport system of Escherichia coli was modified to enhance production of succinate, a highly attractive chemical building block. The engineering comprised the elimination of succinate uptake and the overexpression of succinate export. However, succinate export in Escherichia coli is normally only active under anaerobic conditions and import only under aerobic conditions. Therefore, the gene responsible for succinate import, dctA, was knocked out and the gene coding for succinate export, dcuC, was overexpressed with a constitutive artificial promoter. In the applied batch cultivations, these modifications increased succinate yield and specific production rate more than 50% in a Delta dhA Delta sdhB background (0.16 C-mole/C-mole glucose and 0.17 C-mole/C-mole biomass/h, respectively), but also revealed alternative succinate import proteins, YdjN and YbhI. Mutations in the genes coding for these proteins led to increased growth rates and specific production rates, however, they did not increase succinate yield (e. g. the deletion of ybhI resulted in a growth rate of 0.54 h(-1) and a specific production rate of 0.23 C-mole/C-mole biomass/h).
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
CAPABILITIES, PROTEINS, DATABASE, BACTERIA, metabolic engineering, genetic engineering, E coli, succinate, transport, ESCHERICHIA-COLI, MICROBIAL GENOME ANALYSES, PHOSPHOENOLPYRUVATE, INACTIVATION, FERMENTATION
journal title
GREEN CHEMISTRY
Green Chem.
volume
13
issue
8
pages
2179 - 2186
Web of Science type
Article
Web of Science id
000293518300037
JCR category
CHEMISTRY, MULTIDISCIPLINARY
JCR impact factor
6.32 (2011)
JCR rank
16/149 (2011)
JCR quartile
1 (2011)
ISSN
1463-9262
DOI
10.1039/C1GC15369B
project
Biotechnology for a sustainable economy (Bio-Economy)
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
1853192
handle
http://hdl.handle.net/1854/LU-1853192
date created
2011-07-05 16:44:32
date last changed
2013-02-27 12:59:23
@article{1853192,
  abstract     = {Current climate issues and the ongoing depletion of oil reserves have led to increased attention for biobased production processes. Not only has the production of bio-energy gained interest, but also the production of biochemicals. Succinate is one of those biochemicals. In the presented work, the dicarboxylic acid transport system of Escherichia coli was modified to enhance production of succinate, a highly attractive chemical building block. The engineering comprised the elimination of succinate uptake and the overexpression of succinate export. However, succinate export in Escherichia coli is normally only active under anaerobic conditions and import only under aerobic conditions. Therefore, the gene responsible for succinate import, dctA, was knocked out and the gene coding for succinate export, dcuC, was overexpressed with a constitutive artificial promoter. In the applied batch cultivations, these modifications increased succinate yield and specific production rate more than 50\% in a Delta dhA Delta sdhB background (0.16 C-mole/C-mole glucose and 0.17 C-mole/C-mole biomass/h, respectively), but also revealed alternative succinate import proteins, YdjN and YbhI. Mutations in the genes coding for these proteins led to increased growth rates and specific production rates, however, they did not increase succinate yield (e. g. the deletion of ybhI resulted in a growth rate of 0.54 h(-1) and a specific production rate of 0.23 C-mole/C-mole biomass/h).},
  author       = {Beauprez, Joeri and Foulqui{\'e}-Moreno, Maria R and Maertens, Jo and Van Horen, Ellen and Bekers, Katelijne and Baart, Gino and Cunin, Raymond M and Charlier, Daniel and Heijnen, Joseph J and Soetaert, Wim},
  issn         = {1463-9262},
  journal      = {GREEN CHEMISTRY},
  keyword      = {CAPABILITIES,PROTEINS,DATABASE,BACTERIA,metabolic engineering,genetic engineering,E coli,succinate,transport,ESCHERICHIA-COLI,MICROBIAL GENOME ANALYSES,PHOSPHOENOLPYRUVATE,INACTIVATION,FERMENTATION},
  language     = {eng},
  number       = {8},
  pages        = {2179--2186},
  title        = {Influence of C4-dicarboxylic acid transporters on succinate production},
  url          = {http://dx.doi.org/10.1039/C1GC15369B},
  volume       = {13},
  year         = {2011},
}

Chicago
Beauprez, Joeri, Maria R Foulquié-Moreno, Jo Maertens, Ellen Van Horen, Katelijne Bekers, Gino Baart, Raymond M Cunin, Daniel Charlier, Joseph J Heijnen, and Wim Soetaert. 2011. “Influence of C4-dicarboxylic Acid Transporters on Succinate Production.” Green Chemistry 13 (8): 2179–2186.
APA
Beauprez, J., Foulquié-Moreno, M. R., Maertens, J., Van Horen, E., Bekers, K., Baart, G., Cunin, R. M., et al. (2011). Influence of C4-dicarboxylic acid transporters on succinate production. GREEN CHEMISTRY, 13(8), 2179–2186.
Vancouver
1.
Beauprez J, Foulquié-Moreno MR, Maertens J, Van Horen E, Bekers K, Baart G, et al. Influence of C4-dicarboxylic acid transporters on succinate production. GREEN CHEMISTRY. 2011;13(8):2179–86.
MLA
Beauprez, Joeri, Maria R Foulquié-Moreno, Jo Maertens, et al. “Influence of C4-dicarboxylic Acid Transporters on Succinate Production.” GREEN CHEMISTRY 13.8 (2011): 2179–2186. Print.