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Determination of trace and ultratrace elements in human serum with a double focusing magnetic sector inductively coupled plasma mass spectrometer

Jörgen Riondato, Frank Vanhaecke UGent, Luc Moens UGent and Richard Dams UGent (1997) JOURNAL OF ANALYTICAL ATOMIC SPECTROMETRY. 12(9). p.933-937
abstract
The purpose of this study was to evaluate the potential of ICP-MS with a double focusing instrument for the determination of both spectrally and not spectrally interfered ultratrace elements in human serum after minimised sample pre-treatment and without using any separation or preconcentration techniques, Because of the heavy matrix (6-8% proteins and 1% inorganic compounds) of the material investigated, a higher resolving power than is available with quadrupole instruments was necessary for the determination of some elements in order to eliminate spectral interferences, This was achieved by using ICP-MS apparatus equipped with a double focusing magnetic sector MS, This type of instrument also permits lower detection limits when compared with traditional quadrupole ICP-MS instruments, mainly owing to the low instrumental background values. Determinations were carried out using external calibration and, for most of the elements, also single standard additions as calibration techniques, Both the non-spectrally interfered (Cd, Sn, Ag and U) and the spectrally interfered (Al, Si, P, S, Ti, Cr, Mn, Fe, Cu and Zn) elements were determined in a Second Generation Human Serum Reference Material, Sample preparation consisted in reconstitution of the; freeze-dried material or in microwave digestion, both followed by dilution, In addition, to continuous nebulization, flow injection was also applied as an introduction technique for the determination of some elements in reconstituted serum, The results for the certified elements were all within the certified range except for Cr for which a significantly higher value was obtained, The results of the remaining elements were compared with literature values where they existed, Very low concentrations could tie determined: for instance a concentration as low as 98 ng l(-1) was determined for Ag with a standard deviation of less than 10%, The major difficulty encountered in this study was to keep the blank values sufficiently low, which could only be achieved by using ultra-pure reagents and by working in a clean environment.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (proceedingsPaper)
publication status
published
subject
keyword
spectral interference, ultratrace elements, double focusing inductively coupled plasma mass spectrometry, human serum, flow injection, blank value, ATOMIC-ABSORPTION SPECTROMETRY, POLYATOMIC ION INTERFERENCES, ELECTROTHERMAL VAPORIZATION, SAMPLE INTRODUCTION, BIOLOGICAL SAMPLES, RESOLUTION, REDUCTION, FURNACE, SILICON
journal title
JOURNAL OF ANALYTICAL ATOMIC SPECTROMETRY
J. Anal. At. Spectrom.
volume
12
issue
9
pages
933 - 937
conference name
1997 European winter conference on Plasma Spectrochemistry
conference location
Ghent, Belgium
conference start
1997-01-12
conference end
1997-01-17
Web of Science type
Article
ISSN
0267-9477
DOI
10.1039/a701652b
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
185026
handle
http://hdl.handle.net/1854/LU-185026
date created
2004-01-14 13:41:00
date last changed
2012-11-21 13:27:14
@article{185026,
  abstract     = {The purpose of this study was to evaluate the potential of ICP-MS with a double focusing instrument for the determination of both spectrally and not spectrally interfered ultratrace elements in human serum after minimised sample pre-treatment and without using any separation or preconcentration techniques, Because of the heavy matrix (6-8\% proteins and 1\% inorganic compounds) of the material investigated, a higher resolving power than is available with quadrupole instruments was necessary for the determination of some elements in order to eliminate spectral interferences, This was achieved by using ICP-MS apparatus equipped with a double focusing magnetic sector MS, This type of instrument also permits lower detection limits when compared with traditional quadrupole ICP-MS instruments, mainly owing to the low instrumental background values. Determinations were carried out using external calibration and, for most of the elements, also single standard additions as calibration techniques, Both the non-spectrally interfered (Cd, Sn, Ag and U) and the spectrally interfered (Al, Si, P, S, Ti, Cr, Mn, Fe, Cu and Zn) elements were determined in a Second Generation Human Serum Reference Material, Sample preparation consisted in reconstitution of the; freeze-dried material or in microwave digestion, both followed by dilution, In addition, to continuous nebulization, flow injection was also applied as an introduction technique for the determination of some elements in reconstituted serum, The results for the certified elements were all within the certified range except for Cr for which a significantly higher value was obtained, The results of the remaining elements were compared with literature values where they existed, Very low concentrations could tie determined: for instance a concentration as low as 98 ng l(-1) was determined for Ag with a standard deviation of less than 10\%, The major difficulty encountered in this study was to keep the blank values sufficiently low, which could only be achieved by using ultra-pure reagents and by working in a clean environment.},
  author       = {Riondato, J{\"o}rgen and Vanhaecke, Frank and Moens, Luc and Dams, Richard},
  issn         = {0267-9477},
  journal      = {JOURNAL OF ANALYTICAL ATOMIC SPECTROMETRY},
  keyword      = {spectral interference,ultratrace elements,double focusing inductively coupled plasma mass spectrometry,human serum,flow injection,blank value,ATOMIC-ABSORPTION SPECTROMETRY,POLYATOMIC ION INTERFERENCES,ELECTROTHERMAL VAPORIZATION,SAMPLE INTRODUCTION,BIOLOGICAL SAMPLES,RESOLUTION,REDUCTION,FURNACE,SILICON},
  language     = {eng},
  location     = {Ghent, Belgium},
  number       = {9},
  pages        = {933--937},
  title        = {Determination of trace and ultratrace elements in human serum with a double focusing magnetic sector inductively coupled plasma mass spectrometer},
  url          = {http://dx.doi.org/10.1039/a701652b},
  volume       = {12},
  year         = {1997},
}

Chicago
Riondato, Jörgen, Frank Vanhaecke, Luc Moens, and Richard Dams. 1997. “Determination of Trace and Ultratrace Elements in Human Serum with a Double Focusing Magnetic Sector Inductively Coupled Plasma Mass Spectrometer.” Journal of Analytical Atomic Spectrometry 12 (9): 933–937.
APA
Riondato, J., Vanhaecke, F., Moens, L., & Dams, R. (1997). Determination of trace and ultratrace elements in human serum with a double focusing magnetic sector inductively coupled plasma mass spectrometer. JOURNAL OF ANALYTICAL ATOMIC SPECTROMETRY, 12(9), 933–937. Presented at the 1997 European winter conference on Plasma Spectrochemistry.
Vancouver
1.
Riondato J, Vanhaecke F, Moens L, Dams R. Determination of trace and ultratrace elements in human serum with a double focusing magnetic sector inductively coupled plasma mass spectrometer. JOURNAL OF ANALYTICAL ATOMIC SPECTROMETRY. 1997;12(9):933–7.
MLA
Riondato, Jörgen, Frank Vanhaecke, Luc Moens, et al. “Determination of Trace and Ultratrace Elements in Human Serum with a Double Focusing Magnetic Sector Inductively Coupled Plasma Mass Spectrometer.” JOURNAL OF ANALYTICAL ATOMIC SPECTROMETRY 12.9 (1997): 933–937. Print.