Advanced search
1 file | 1.06 MB

Oleosin gene expression in Arabidopsis thaliana tapetum coincides with accumulation of lipids in plastids and cytoplasmic bodies

Author
Organization
Abstract
Oleosins are alkaline proteins found on the surface of oil bodies in plants. They serve a structural role in stabilizing the triacylglycerols of the oil bodies together with the phos pholipid layer. We studied the spatial and temporal expression pat;ern of three putative oleosin genes atgrp-6, atgrp-7, and atgrp-8, in anthers of Arabidopsis thaliana using RNA transcript in situ hybridization. For all three genes, transcripts were found within the tapetal cells during the process of senescence. To identify a putative function for corresponding proteins, the tapetum was characterized ultrastructurally by electron microscopy and analyzed cytochemically for the presence of unsaturated lipids and basic proteins. TWO lipid storage organelles were identified: elaioplastids and lipid protein bodies. The accumulation of lipids in these two organelles strictly coincides with oleosin gene expression in the tapetum of A. rhaliana. No typical oil body was observed during tapetum development. A putative function of oleosins in tapetal cells is discussed.
Keywords
OIL BODIES, BRASSICA-NAPUS, Arabidopsis thaliana, tapetum, senescence, oleosin, lipid protein body, glycine-rich protein, POLLEN, SEEDS, VISUALIZATION, LOCALIZATION, IMIDAZOLE, PATTERNS, OSMIUM-TETROXIDE, PROTEINS

Downloads

  • (...).pdf
    • full text
    • |
    • UGent only
    • |
    • PDF
    • |
    • 1.06 MB

Citation

Please use this url to cite or link to this publication:

Chicago
Alves Ferreira, Marcio, Janice de Almeida Engler, Flávio Costa Miguens, Marc Van Montagu, Gilbert Engler, and Dulce De Oliveira. 1997. “Oleosin Gene Expression in Arabidopsis Thaliana Tapetum Coincides with Accumulation of Lipids in Plastids and Cytoplasmic Bodies.” Plant Physiology and Biochemistry 35 (9): 729–739.
APA
Alves Ferreira, M., de Almeida Engler, J., Miguens, F. C., Van Montagu, M., Engler, G., & De Oliveira, D. (1997). Oleosin gene expression in Arabidopsis thaliana tapetum coincides with accumulation of lipids in plastids and cytoplasmic bodies. PLANT PHYSIOLOGY AND BIOCHEMISTRY, 35(9), 729–739.
Vancouver
1.
Alves Ferreira M, de Almeida Engler J, Miguens FC, Van Montagu M, Engler G, De Oliveira D. Oleosin gene expression in Arabidopsis thaliana tapetum coincides with accumulation of lipids in plastids and cytoplasmic bodies. PLANT PHYSIOLOGY AND BIOCHEMISTRY. 1997;35(9):729–39.
MLA
Alves Ferreira, Marcio, Janice de Almeida Engler, Flávio Costa Miguens, et al. “Oleosin Gene Expression in Arabidopsis Thaliana Tapetum Coincides with Accumulation of Lipids in Plastids and Cytoplasmic Bodies.” PLANT PHYSIOLOGY AND BIOCHEMISTRY 35.9 (1997): 729–739. Print.
@article{185021,
  abstract     = {Oleosins are alkaline proteins found on the surface of oil bodies in plants. They serve a structural role in stabilizing the triacylglycerols of the oil bodies together with the phos pholipid layer. We studied the spatial and temporal expression pat;ern of three putative oleosin genes atgrp-6, atgrp-7, and atgrp-8, in anthers of Arabidopsis thaliana using RNA transcript in situ hybridization. For all three genes, transcripts were found within the tapetal cells during the process of senescence. To identify a putative function for corresponding proteins, the tapetum was characterized ultrastructurally by electron microscopy and analyzed cytochemically for the presence of unsaturated lipids and basic proteins. TWO lipid storage organelles were identified: elaioplastids and lipid protein bodies. The accumulation of lipids in these two organelles strictly coincides with oleosin gene expression in the tapetum of A. rhaliana. No typical oil body was observed during tapetum development. A putative function of oleosins in tapetal cells is discussed.},
  author       = {Alves Ferreira, Marcio and de Almeida Engler, Janice and Miguens, Flávio Costa and Van Montagu, Marc and Engler, Gilbert and De Oliveira, Dulce},
  issn         = {0981-9428},
  journal      = {PLANT PHYSIOLOGY AND BIOCHEMISTRY},
  keywords     = {OIL BODIES,BRASSICA-NAPUS,Arabidopsis thaliana,tapetum,senescence,oleosin,lipid protein body,glycine-rich protein,POLLEN,SEEDS,VISUALIZATION,LOCALIZATION,IMIDAZOLE,PATTERNS,OSMIUM-TETROXIDE,PROTEINS},
  language     = {eng},
  number       = {9},
  pages        = {729--739},
  title        = {Oleosin gene expression in Arabidopsis thaliana tapetum coincides with accumulation of lipids in plastids and cytoplasmic bodies},
  volume       = {35},
  year         = {1997},
}