Advanced search

Semi-automated micronucleus scoring in cytokinesis-blocked lymphocytes after irradiation

Hubert Thierens (UGent) , Anne Vral (UGent) , F De Scheerder, Leo De Ridder (UGent) and A Tates
Author
Organization
Abstract
A semi-automated scoring procedure for the cytokinesis-block micronucleus assay based on computerized image analysis was validated by comparing results of the automated analysis with those from manual analysis by three experienced scorers of micronuclei. Each object selected by the computer system was also relocated and visually inspected. The validation was based on slides of blood samples after in vitro irradiation with X-rays in the low-dose range up to 2 Gy. Up to 1 Gy a very satisfactory agreement between manual and semi-automated scoring was obtained. At 2 Gy the micronucleus yields obtained by the semi-automated scoring were systematically below the manual data due to a small percentage of false-negatives (3%). Using the appropriate dose-response relationship, the validation study indicates that the results obtained by computerized image analysis are comparable to manual scoring with respect to accuracy. The computerized assay is superior regarding reproducibility. At present, semi-automated scoring of micronuclei is practically feasible for a limited number of slides. For large-scare applications of the semi-automated micronucleus assay in the field of radiation protection an increase of the speed of the system is necessary.
Keywords
DETAILED DESCRIPTION, RADIATION-INDUCED MICRONUCLEI, IMAGE-ANALYSIS, ASSAY

Citation

Please use this url to cite or link to this publication:

Chicago
Thierens, Hubert, Anne Vral, F De Scheerder, Leo De Ridder, and A Tates. 1997. “Semi-automated Micronucleus Scoring in Cytokinesis-blocked Lymphocytes After Irradiation.” International Journal of Radiation Biology 72 (3): 319–324.
APA
Thierens, H., Vral, A., De Scheerder, F., De Ridder, L., & Tates, A. (1997). Semi-automated micronucleus scoring in cytokinesis-blocked lymphocytes after irradiation. INTERNATIONAL JOURNAL OF RADIATION BIOLOGY, 72(3), 319–324.
Vancouver
1.
Thierens H, Vral A, De Scheerder F, De Ridder L, Tates A. Semi-automated micronucleus scoring in cytokinesis-blocked lymphocytes after irradiation. INTERNATIONAL JOURNAL OF RADIATION BIOLOGY. 1997;72(3):319–24.
MLA
Thierens, Hubert, Anne Vral, F De Scheerder, et al. “Semi-automated Micronucleus Scoring in Cytokinesis-blocked Lymphocytes After Irradiation.” INTERNATIONAL JOURNAL OF RADIATION BIOLOGY 72.3 (1997): 319–324. Print.
@article{184974,
  abstract     = {A semi-automated scoring procedure for the cytokinesis-block micronucleus assay based on computerized image analysis was validated by comparing results of the automated analysis with those from manual analysis by three experienced scorers of micronuclei. Each object selected by the computer system was also relocated and visually inspected. The validation was based on slides of blood samples after in vitro irradiation with X-rays in the low-dose range up to 2 Gy. Up to 1 Gy a very satisfactory agreement between manual and semi-automated scoring was obtained. At 2 Gy the micronucleus yields obtained by the semi-automated scoring were systematically below the manual data due to a small percentage of false-negatives (3\%). Using the appropriate dose-response relationship, the validation study indicates that the results obtained by computerized image analysis are comparable to manual scoring with respect to accuracy. The computerized assay is superior regarding reproducibility. At present, semi-automated scoring of micronuclei is practically feasible for a limited number of slides. For large-scare applications of the semi-automated micronucleus assay in the field of radiation protection an increase of the speed of the system is necessary.},
  author       = {Thierens, Hubert and Vral, Anne and De Scheerder, F and De Ridder, Leo and Tates, A},
  issn         = {0955-3002},
  journal      = {INTERNATIONAL JOURNAL OF RADIATION BIOLOGY},
  keyword      = {DETAILED DESCRIPTION,RADIATION-INDUCED MICRONUCLEI,IMAGE-ANALYSIS,ASSAY},
  language     = {eng},
  number       = {3},
  pages        = {319--324},
  title        = {Semi-automated micronucleus scoring in cytokinesis-blocked lymphocytes after irradiation},
  url          = {http://dx.doi.org/10.1080/095530097143310},
  volume       = {72},
  year         = {1997},
}

Altmetric
View in Altmetric