FISH analysis of Lactobacillus biofilms in the gastrointestinal tract of different hosts
- Author
- S Lebeer, TLA Verhoeven, IJJ Claes, G De Hertogh, S Vermeire, J Buyse, Filip Van Immerseel (UGent) , J Vanderleyden and SCJ De Keersmaecker
- Organization
- Abstract
- Aims: To investigate the spatial organization of endogenous and exogenously applied Lactobacillus communities at specific locations in the adult gastrointestinal tract of different hosts. Methods and Results: Samples of the human, murine and avian gastrointestinal tract of subjects that received or not received a Lactobacillus probiotic were analysed by fluorescence in situ hybridization (FISH) with rRNA-targeted probes. High levels of endogenous lactobacilli were observed on the nonsecretory, stratified squamous epithelia present in the forestomach of mice and crop of chickens, respectively. These epithelial associations showed characteristics of bacterial biofilms, i.e. bacteria attached to a surface and embedded in a matrix of extracellular polymeric substances. In other regions of the analysed intestines, lactobacilli seemed to occur mainly as dispersed bacterial cells or as microcolonies. Exogenous administration of a Lactobacillus probiotic did increase the levels of loosely adherent Lactobacillus cells detected. However, the probiotic strains were unable to establish themselves inside the gastrointestinal biofilms. Conclusions: Gastrointestinal biofilms of lactobacilli occur only in specific niches in certain hosts, such as the murine forestomach and avian crop. Significance and Impact of the Study: Biofilm formation by lactobacilli in specific parts of animal gastrointestinal tracts was documented for the first time by FISH.
- Keywords
- LACTIC-ACID BACTERIA, MUCOSA, RHAMNOSUS GG, FUNCTIONAL-ANALYSIS, IDENTIFICATION, HYBRIDIZATION, INTESTINE, PROBIOTICS, MICROFLORA, DISEASE, probiotics, Lactobacillus, intestinal microbiology
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Citation
Please use this url to cite or link to this publication: http://hdl.handle.net/1854/LU-1849600
- MLA
- Lebeer, S., et al. “FISH Analysis of Lactobacillus Biofilms in the Gastrointestinal Tract of Different Hosts.” LETTERS IN APPLIED MICROBIOLOGY, vol. 52, no. 3, 2011, pp. 220–26, doi:10.1111/j.1472-765X.2010.02994.x.
- APA
- Lebeer, S., Verhoeven, T., Claes, I., De Hertogh, G., Vermeire, S., Buyse, J., … De Keersmaecker, S. (2011). FISH analysis of Lactobacillus biofilms in the gastrointestinal tract of different hosts. LETTERS IN APPLIED MICROBIOLOGY, 52(3), 220–226. https://doi.org/10.1111/j.1472-765X.2010.02994.x
- Chicago author-date
- Lebeer, S, TLA Verhoeven, IJJ Claes, G De Hertogh, S Vermeire, J Buyse, Filip Van Immerseel, J Vanderleyden, and SCJ De Keersmaecker. 2011. “FISH Analysis of Lactobacillus Biofilms in the Gastrointestinal Tract of Different Hosts.” LETTERS IN APPLIED MICROBIOLOGY 52 (3): 220–26. https://doi.org/10.1111/j.1472-765X.2010.02994.x.
- Chicago author-date (all authors)
- Lebeer, S, TLA Verhoeven, IJJ Claes, G De Hertogh, S Vermeire, J Buyse, Filip Van Immerseel, J Vanderleyden, and SCJ De Keersmaecker. 2011. “FISH Analysis of Lactobacillus Biofilms in the Gastrointestinal Tract of Different Hosts.” LETTERS IN APPLIED MICROBIOLOGY 52 (3): 220–226. doi:10.1111/j.1472-765X.2010.02994.x.
- Vancouver
- 1.Lebeer S, Verhoeven T, Claes I, De Hertogh G, Vermeire S, Buyse J, et al. FISH analysis of Lactobacillus biofilms in the gastrointestinal tract of different hosts. LETTERS IN APPLIED MICROBIOLOGY. 2011;52(3):220–6.
- IEEE
- [1]S. Lebeer et al., “FISH analysis of Lactobacillus biofilms in the gastrointestinal tract of different hosts,” LETTERS IN APPLIED MICROBIOLOGY, vol. 52, no. 3, pp. 220–226, 2011.
@article{1849600,
abstract = {{Aims: To investigate the spatial organization of endogenous and exogenously applied Lactobacillus communities at specific locations in the adult gastrointestinal tract of different hosts.
Methods and Results: Samples of the human, murine and avian gastrointestinal tract of subjects that received or not received a Lactobacillus probiotic were analysed by fluorescence in situ hybridization (FISH) with rRNA-targeted probes. High levels of endogenous lactobacilli were observed on the nonsecretory, stratified squamous epithelia present in the forestomach of mice and crop of chickens, respectively. These epithelial associations showed characteristics of bacterial biofilms, i.e. bacteria attached to a surface and embedded in a matrix of extracellular polymeric substances. In other regions of the analysed intestines, lactobacilli seemed to occur mainly as dispersed bacterial cells or as microcolonies. Exogenous administration of a Lactobacillus probiotic did increase the levels of loosely adherent Lactobacillus cells detected. However, the probiotic strains were unable to establish themselves inside the gastrointestinal biofilms.
Conclusions: Gastrointestinal biofilms of lactobacilli occur only in specific niches in certain hosts, such as the murine forestomach and avian crop.
Significance and Impact of the Study: Biofilm formation by lactobacilli in specific parts of animal gastrointestinal tracts was documented for the first time by FISH.}},
author = {{Lebeer, S and Verhoeven, TLA and Claes, IJJ and De Hertogh, G and Vermeire, S and Buyse, J and Van Immerseel, Filip and Vanderleyden, J and De Keersmaecker, SCJ}},
issn = {{0266-8254}},
journal = {{LETTERS IN APPLIED MICROBIOLOGY}},
keywords = {{LACTIC-ACID BACTERIA,MUCOSA,RHAMNOSUS GG,FUNCTIONAL-ANALYSIS,IDENTIFICATION,HYBRIDIZATION,INTESTINE,PROBIOTICS,MICROFLORA,DISEASE,probiotics,Lactobacillus,intestinal microbiology}},
language = {{eng}},
number = {{3}},
pages = {{220--226}},
title = {{FISH analysis of Lactobacillus biofilms in the gastrointestinal tract of different hosts}},
url = {{http://doi.org/10.1111/j.1472-765X.2010.02994.x}},
volume = {{52}},
year = {{2011}},
}
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