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Considerations on comprehensive and off-line supercritical fluid chromatography x reversed-phase liquid chromatography for the analysis of triacylglycerols in fish oil

Isabelle François UGent, Alberto Dos Santos Pereira UGent and Patrick Sandra UGent (2010) JOURNAL OF SEPARATION SCIENCE. 33(10). p.1504-1512
abstract
The separation of the triacylglycerols in fish oil was performed by comprehensive and off-line supercritical fluid chromatography combined with RP-LC. The first dimension consisted of two serially coupled silver-ion (SI)-loaded columns operated with a supercritical mobile phase (supercritical fluid chromatography, SFC) in both the cases, whereas the second dimension was performed in non-aqueous RP mode (NARP-LC) on a 10-cm monolithic octadecyl silica (ODS) or a 45-cm long ODS column packed with 1.8 mu m particles for the comprehensive and off-line separations, respectively. Despite the outstanding performance of the SI-SFC x NARP-LC interface, the high complexity of the sample rendered the online separation far from complete. The off-line approach gave much better separation mainly because of the higher peak capacity of the second-dimension column, but even in this case, the use of MS was mandatory to elucidate the different triacylglycerols in fish oil. The disadvantage of the off-line procedure was the long analysis time.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
TRIGLYCERIDES
journal title
JOURNAL OF SEPARATION SCIENCE
J. Sep. Sci.
volume
33
issue
10
pages
1504 - 1512
Web of Science type
Article
Web of Science id
000278572000018
JCR category
CHEMISTRY, ANALYTICAL
JCR impact factor
2.631 (2010)
JCR rank
23/71 (2010)
JCR quartile
2 (2010)
ISSN
1615-9306
DOI
10.1002/jssc.201000044
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
1849548
handle
http://hdl.handle.net/1854/LU-1849548
date created
2011-06-30 14:53:59
date last changed
2011-07-05 12:56:14
@article{1849548,
  abstract     = {The separation of the triacylglycerols in fish oil was performed by comprehensive and off-line supercritical fluid chromatography combined with RP-LC. The first dimension consisted of two serially coupled silver-ion (SI)-loaded columns operated with a supercritical mobile phase (supercritical fluid chromatography, SFC) in both the cases, whereas the second dimension was performed in non-aqueous RP mode (NARP-LC) on a 10-cm monolithic octadecyl silica (ODS) or a 45-cm long ODS column packed with 1.8 mu m particles for the comprehensive and off-line separations, respectively. Despite the outstanding performance of the SI-SFC x NARP-LC interface, the high complexity of the sample rendered the online separation far from complete. The off-line approach gave much better separation mainly because of the higher peak capacity of the second-dimension column, but even in this case, the use of MS was mandatory to elucidate the different triacylglycerols in fish oil. The disadvantage of the off-line procedure was the long analysis time.},
  author       = {Fran\c{c}ois, Isabelle and Dos Santos Pereira, Alberto and Sandra, Patrick},
  issn         = {1615-9306},
  journal      = {JOURNAL OF SEPARATION SCIENCE},
  keyword      = {TRIGLYCERIDES},
  language     = {eng},
  number       = {10},
  pages        = {1504--1512},
  title        = {Considerations on comprehensive and off-line supercritical fluid chromatography x reversed-phase liquid chromatography for the analysis of triacylglycerols in fish oil},
  url          = {http://dx.doi.org/10.1002/jssc.201000044},
  volume       = {33},
  year         = {2010},
}

Chicago
François, Isabelle, Alberto Dos Santos Pereira, and Patrick Sandra. 2010. “Considerations on Comprehensive and Off-line Supercritical Fluid Chromatography x Reversed-phase Liquid Chromatography for the Analysis of Triacylglycerols in Fish Oil.” Journal of Separation Science 33 (10): 1504–1512.
APA
François, I., Dos Santos Pereira, A., & Sandra, P. (2010). Considerations on comprehensive and off-line supercritical fluid chromatography x reversed-phase liquid chromatography for the analysis of triacylglycerols in fish oil. JOURNAL OF SEPARATION SCIENCE, 33(10), 1504–1512.
Vancouver
1.
François I, Dos Santos Pereira A, Sandra P. Considerations on comprehensive and off-line supercritical fluid chromatography x reversed-phase liquid chromatography for the analysis of triacylglycerols in fish oil. JOURNAL OF SEPARATION SCIENCE. 2010;33(10):1504–12.
MLA
François, Isabelle, Alberto Dos Santos Pereira, and Patrick Sandra. “Considerations on Comprehensive and Off-line Supercritical Fluid Chromatography x Reversed-phase Liquid Chromatography for the Analysis of Triacylglycerols in Fish Oil.” JOURNAL OF SEPARATION SCIENCE 33.10 (2010): 1504–1512. Print.