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Considerations on comprehensive and off-line supercritical fluid chromatography x reversed-phase liquid chromatography for the analysis of triacylglycerols in fish oil

(2010) JOURNAL OF SEPARATION SCIENCE. 33(10). p.1504-1512
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Abstract
The separation of the triacylglycerols in fish oil was performed by comprehensive and off-line supercritical fluid chromatography combined with RP-LC. The first dimension consisted of two serially coupled silver-ion (SI)-loaded columns operated with a supercritical mobile phase (supercritical fluid chromatography, SFC) in both the cases, whereas the second dimension was performed in non-aqueous RP mode (NARP-LC) on a 10-cm monolithic octadecyl silica (ODS) or a 45-cm long ODS column packed with 1.8 mu m particles for the comprehensive and off-line separations, respectively. Despite the outstanding performance of the SI-SFC x NARP-LC interface, the high complexity of the sample rendered the online separation far from complete. The off-line approach gave much better separation mainly because of the higher peak capacity of the second-dimension column, but even in this case, the use of MS was mandatory to elucidate the different triacylglycerols in fish oil. The disadvantage of the off-line procedure was the long analysis time.
Keywords
TRIGLYCERIDES

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Chicago
François, Isabelle, Alberto Dos Santos Pereira, and Patrick Sandra. 2010. “Considerations on Comprehensive and Off-line Supercritical Fluid Chromatography x Reversed-phase Liquid Chromatography for the Analysis of Triacylglycerols in Fish Oil.” Journal of Separation Science 33 (10): 1504–1512.
APA
François, I., Dos Santos Pereira, A., & Sandra, P. (2010). Considerations on comprehensive and off-line supercritical fluid chromatography x reversed-phase liquid chromatography for the analysis of triacylglycerols in fish oil. JOURNAL OF SEPARATION SCIENCE, 33(10), 1504–1512.
Vancouver
1.
François I, Dos Santos Pereira A, Sandra P. Considerations on comprehensive and off-line supercritical fluid chromatography x reversed-phase liquid chromatography for the analysis of triacylglycerols in fish oil. JOURNAL OF SEPARATION SCIENCE. 2010;33(10):1504–12.
MLA
François, Isabelle, Alberto Dos Santos Pereira, and Patrick Sandra. “Considerations on Comprehensive and Off-line Supercritical Fluid Chromatography x Reversed-phase Liquid Chromatography for the Analysis of Triacylglycerols in Fish Oil.” JOURNAL OF SEPARATION SCIENCE 33.10 (2010): 1504–1512. Print.
@article{1849548,
  abstract     = {The separation of the triacylglycerols in fish oil was performed by comprehensive and off-line supercritical fluid chromatography combined with RP-LC. The first dimension consisted of two serially coupled silver-ion (SI)-loaded columns operated with a supercritical mobile phase (supercritical fluid chromatography, SFC) in both the cases, whereas the second dimension was performed in non-aqueous RP mode (NARP-LC) on a 10-cm monolithic octadecyl silica (ODS) or a 45-cm long ODS column packed with 1.8 mu m particles for the comprehensive and off-line separations, respectively. Despite the outstanding performance of the SI-SFC x NARP-LC interface, the high complexity of the sample rendered the online separation far from complete. The off-line approach gave much better separation mainly because of the higher peak capacity of the second-dimension column, but even in this case, the use of MS was mandatory to elucidate the different triacylglycerols in fish oil. The disadvantage of the off-line procedure was the long analysis time.},
  author       = {Fran\c{c}ois, Isabelle and Dos Santos Pereira, Alberto and Sandra, Patrick},
  issn         = {1615-9306},
  journal      = {JOURNAL OF SEPARATION SCIENCE},
  language     = {eng},
  number       = {10},
  pages        = {1504--1512},
  title        = {Considerations on comprehensive and off-line supercritical fluid chromatography x reversed-phase liquid chromatography for the analysis of triacylglycerols in fish oil},
  url          = {http://dx.doi.org/10.1002/jssc.201000044},
  volume       = {33},
  year         = {2010},
}

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