Considerations on comprehensive and off-line supercritical fluid chromatography x reversed-phase liquid chromatography for the analysis of triacylglycerols in fish oil
- Author
- Isabelle François (UGent) , Alberto Dos Santos Pereira (UGent) and Patrick Sandra (UGent)
- Organization
- Abstract
- The separation of the triacylglycerols in fish oil was performed by comprehensive and off-line supercritical fluid chromatography combined with RP-LC. The first dimension consisted of two serially coupled silver-ion (SI)-loaded columns operated with a supercritical mobile phase (supercritical fluid chromatography, SFC) in both the cases, whereas the second dimension was performed in non-aqueous RP mode (NARP-LC) on a 10-cm monolithic octadecyl silica (ODS) or a 45-cm long ODS column packed with 1.8 mu m particles for the comprehensive and off-line separations, respectively. Despite the outstanding performance of the SI-SFC x NARP-LC interface, the high complexity of the sample rendered the online separation far from complete. The off-line approach gave much better separation mainly because of the higher peak capacity of the second-dimension column, but even in this case, the use of MS was mandatory to elucidate the different triacylglycerols in fish oil. The disadvantage of the off-line procedure was the long analysis time.
- Keywords
- TRIGLYCERIDES
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Please use this url to cite or link to this publication: http://hdl.handle.net/1854/LU-1849548
- MLA
- François, Isabelle, et al. “Considerations on Comprehensive and Off-Line Supercritical Fluid Chromatography x Reversed-Phase Liquid Chromatography for the Analysis of Triacylglycerols in Fish Oil.” JOURNAL OF SEPARATION SCIENCE, vol. 33, no. 10, 2010, pp. 1504–12, doi:10.1002/jssc.201000044.
- APA
- François, I., Dos Santos Pereira, A., & Sandra, P. (2010). Considerations on comprehensive and off-line supercritical fluid chromatography x reversed-phase liquid chromatography for the analysis of triacylglycerols in fish oil. JOURNAL OF SEPARATION SCIENCE, 33(10), 1504–1512. https://doi.org/10.1002/jssc.201000044
- Chicago author-date
- François, Isabelle, Alberto Dos Santos Pereira, and Patrick Sandra. 2010. “Considerations on Comprehensive and Off-Line Supercritical Fluid Chromatography x Reversed-Phase Liquid Chromatography for the Analysis of Triacylglycerols in Fish Oil.” JOURNAL OF SEPARATION SCIENCE 33 (10): 1504–12. https://doi.org/10.1002/jssc.201000044.
- Chicago author-date (all authors)
- François, Isabelle, Alberto Dos Santos Pereira, and Patrick Sandra. 2010. “Considerations on Comprehensive and Off-Line Supercritical Fluid Chromatography x Reversed-Phase Liquid Chromatography for the Analysis of Triacylglycerols in Fish Oil.” JOURNAL OF SEPARATION SCIENCE 33 (10): 1504–1512. doi:10.1002/jssc.201000044.
- Vancouver
- 1.François I, Dos Santos Pereira A, Sandra P. Considerations on comprehensive and off-line supercritical fluid chromatography x reversed-phase liquid chromatography for the analysis of triacylglycerols in fish oil. JOURNAL OF SEPARATION SCIENCE. 2010;33(10):1504–12.
- IEEE
- [1]I. François, A. Dos Santos Pereira, and P. Sandra, “Considerations on comprehensive and off-line supercritical fluid chromatography x reversed-phase liquid chromatography for the analysis of triacylglycerols in fish oil,” JOURNAL OF SEPARATION SCIENCE, vol. 33, no. 10, pp. 1504–1512, 2010.
@article{1849548,
abstract = {{The separation of the triacylglycerols in fish oil was performed by comprehensive and off-line supercritical fluid chromatography combined with RP-LC. The first dimension consisted of two serially coupled silver-ion (SI)-loaded columns operated with a supercritical mobile phase (supercritical fluid chromatography, SFC) in both the cases, whereas the second dimension was performed in non-aqueous RP mode (NARP-LC) on a 10-cm monolithic octadecyl silica (ODS) or a 45-cm long ODS column packed with 1.8 mu m particles for the comprehensive and off-line separations, respectively. Despite the outstanding performance of the SI-SFC x NARP-LC interface, the high complexity of the sample rendered the online separation far from complete. The off-line approach gave much better separation mainly because of the higher peak capacity of the second-dimension column, but even in this case, the use of MS was mandatory to elucidate the different triacylglycerols in fish oil. The disadvantage of the off-line procedure was the long analysis time.}},
author = {{François, Isabelle and Dos Santos Pereira, Alberto and Sandra, Patrick}},
issn = {{1615-9306}},
journal = {{JOURNAL OF SEPARATION SCIENCE}},
keywords = {{TRIGLYCERIDES}},
language = {{eng}},
number = {{10}},
pages = {{1504--1512}},
title = {{Considerations on comprehensive and off-line supercritical fluid chromatography x reversed-phase liquid chromatography for the analysis of triacylglycerols in fish oil}},
url = {{http://dx.doi.org/10.1002/jssc.201000044}},
volume = {{33}},
year = {{2010}},
}
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