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Variation of inflammatory dynamics and mediators in primiparous cows after intramammary challenge with Escherichia coli

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Abstract
The objective of the current study was to investigate (i) the outcome of experimentally induced Escherichia coli mastitis in primiparous cows during early lactation in relation with production of eicosanoids and inflammatory indicators, and (ii) the validity of thermography to evaluate temperature changes on udder skin surface after experimentally induced E. coli mastitis. Nine primiparous Holstein Friesian cows were inoculated 24 +/- 6 days (d) after parturition in both left quarters with E. coli P4 serotype O32:H37. Blood and milk samples were collected before and after challenge with E. coli. The infrared images were taken from the caudal view of the udder following challenge with E. coli. No relationship was detected between severity of mastitis and changes of thromboxane B2 (TXB2), leukotriene B4 (LTB4) and lipoxin A4 (LXA4). However, prostaglandin E2 (PGE2) was related to systemic disease severity during E. coli mastitis. Moreover, reduced somatic cell count (SCC), fewer circulating basophils, increased concentration of tumor necrosis factor-alpha (TNF-alpha) and higher milk sodium and lower milk potassium concentrations were related to systemic disease severity. The thermal camera was capable of detecting 2-3 degrees C temperature changes on udder skin surface of cows inoculated with E. coli. Peak of udder skin temperature occurred after peak of rectal temperature and appearance of local signs of induced E. coli mastitis. Although infrared thermography was a successful method for detecting the changes in udder skin surface temperature following intramammary challenge with E. coli, it did not show to be a promising tool for early detection of mastitis.
Keywords
MILK, LEUKOCYTES, EXPRESSION, PHAGOCYTOSIS, BOVINE MASTITIS, INDUCED MASTITIS, DAIRY-COWS, MAMMARY BLOOD-FLOW, ARACHIDONIC-ACID METABOLITES, RESPIRATORY BURST ACTIVITY

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Chicago
Pezeshki, Adel, Philippe Stordeur, Hugues Wallemacq, Frederic Schynts, Mieke Stevens, Philippe Boutet, Luc Peelman, et al. 2011. “Variation of Inflammatory Dynamics and Mediators in Primiparous Cows After Intramammary Challenge with Escherichia Coli.” Veterinary Research 42.
APA
Pezeshki, A., Stordeur, P., Wallemacq, H., Schynts, F., Stevens, M., Boutet, P., Peelman, L., et al. (2011). Variation of inflammatory dynamics and mediators in primiparous cows after intramammary challenge with Escherichia coli. VETERINARY RESEARCH, 42.
Vancouver
1.
Pezeshki A, Stordeur P, Wallemacq H, Schynts F, Stevens M, Boutet P, et al. Variation of inflammatory dynamics and mediators in primiparous cows after intramammary challenge with Escherichia coli. VETERINARY RESEARCH. 2011;42.
MLA
Pezeshki, Adel, Philippe Stordeur, Hugues Wallemacq, et al. “Variation of Inflammatory Dynamics and Mediators in Primiparous Cows After Intramammary Challenge with Escherichia Coli.” VETERINARY RESEARCH 42 (2011): n. pag. Print.
@article{1849324,
  abstract     = {The objective of the current study was to investigate (i) the outcome of experimentally induced Escherichia coli mastitis in primiparous cows during early lactation in relation with production of eicosanoids and inflammatory indicators, and (ii) the validity of thermography to evaluate temperature changes on udder skin surface after experimentally induced E. coli mastitis. Nine primiparous Holstein Friesian cows were inoculated 24 +/- 6 days (d) after parturition in both left quarters with E. coli P4 serotype O32:H37. Blood and milk samples were collected before and after challenge with E. coli. The infrared images were taken from the caudal view of the udder following challenge with E. coli. No relationship was detected between severity of mastitis and changes of thromboxane B2 (TXB2), leukotriene B4 (LTB4) and lipoxin A4 (LXA4). However, prostaglandin E2 (PGE2) was related to systemic disease severity during E. coli mastitis. Moreover, reduced somatic cell count (SCC), fewer circulating basophils, increased concentration of tumor necrosis factor-alpha (TNF-alpha) and higher milk sodium and lower milk potassium concentrations were related to systemic disease severity. The thermal camera was capable of detecting 2-3 degrees C temperature changes on udder skin surface of cows inoculated with E. coli. Peak of udder skin temperature occurred after peak of rectal temperature and appearance of local signs of induced E. coli mastitis. Although infrared thermography was a successful method for detecting the changes in udder skin surface temperature following intramammary challenge with E. coli, it did not show to be a promising tool for early detection of mastitis.},
  articleno    = {15},
  author       = {Pezeshki, Adel and Stordeur, Philippe and Wallemacq, Hugues and Schynts, Frederic and Stevens, Mieke and Boutet, Philippe and Peelman, Luc and De Spiegeleer, Bart and Duchateau, Luc and Bureau, Fabrice and Burvenich, Christian},
  issn         = {0928-4249},
  journal      = {VETERINARY RESEARCH},
  keyword      = {MILK,LEUKOCYTES,EXPRESSION,PHAGOCYTOSIS,BOVINE MASTITIS,INDUCED MASTITIS,DAIRY-COWS,MAMMARY BLOOD-FLOW,ARACHIDONIC-ACID METABOLITES,RESPIRATORY BURST ACTIVITY},
  language     = {eng},
  pages        = {10},
  title        = {Variation of inflammatory dynamics and mediators in primiparous cows after intramammary challenge with Escherichia coli},
  url          = {http://dx.doi.org/10.1186/1297-9716-42-15},
  volume       = {42},
  year         = {2011},
}

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