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Catalase is a sink for H₂O₂ and is indispensable for stress defence in C₃ plants

(1997) EMBO JOURNAL. 16(16). p.4806-4816
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Abstract
Hydrogen peroxide (H2O2) has been implicated in many stress conditions, Control of H2O2 levels is complex and dissection of mechanisms generating and relieving H2O2 stress is difficult, particularly in intact plants, We have used transgenic tobacco with similar to 10% wild-type catalase activity to study the role of catalase and effects of H2O2 stress in plants, Catalase-deficient plants showed no visible disorders at low Light, but in elevated light rapidly developed white necrotic lesions on the leaves, Lesion formation required photorespiratory activity since damage was prevented under elevated CO2, Accumulation of H2O2 was not detected during leaf necrosis, Alternative H2O2-scavenging mechanisms may have compensated for reduced catalase activity, as shown by increased ascorbate peroxidase and glutathione peroxidase levels, Leaf necrosis correlated with accumulation of oxidized glutathione and a 4-fold decrease in ascorbate, indicating that catalase is critical for maintaining the redox balance during oxidative stress, Such control may not be limited to peroxisomal H2O2 production, Catalase functions as a cellular sink for H2O2, as evidenced by complementation of catalase deficiency by exogenous catalase, and comparison of catalase-deficient and control leaf discs in removing external H2O2. Stress analysis revealed increased susceptibility-of catalase-deficient plants to paraquat, salt and ozone, but not to chilling.
Keywords
ASCORBATE PEROXIDASE, GLUTATHIONE-REDUCTASE, SIGNAL-TRANSDUCTION, DEFICIENT MUTANT, HYDROGEN-PEROXIDE, TRANSGENIC TOBACCO, ascorbate-glutathione cycle, ozone stress, photorespiration, redox control, salt stress, DISEASE RESISTANCE RESPONSE, HORDEUM-VULGARE-L, OXIDATIVE STRESS, SUPEROXIDE-DISMUTASE

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Chicago
Willekens, Hilde, Sangpen Chamnongpol, Mark W Davey, Martina Schraudner, Christian Langebartels, Marc Van Montagu, Dirk Inzé, and Wim Van Camp. 1997. “Catalase Is a Sink for H₂O₂ and Is Indispensable for Stress Defence in C₃ Plants.” Embo Journal 16 (16): 4806–4816.
APA
Willekens, H., Chamnongpol, S., Davey, M. W., Schraudner, M., Langebartels, C., Van Montagu, M., Inzé, D., et al. (1997). Catalase is a sink for H₂O₂ and is indispensable for stress defence in C₃ plants. EMBO JOURNAL, 16(16), 4806–4816.
Vancouver
1.
Willekens H, Chamnongpol S, Davey MW, Schraudner M, Langebartels C, Van Montagu M, et al. Catalase is a sink for H₂O₂ and is indispensable for stress defence in C₃ plants. EMBO JOURNAL. 1997;16(16):4806–16.
MLA
Willekens, Hilde, Sangpen Chamnongpol, Mark W Davey, et al. “Catalase Is a Sink for H₂O₂ and Is Indispensable for Stress Defence in C₃ Plants.” EMBO JOURNAL 16.16 (1997): 4806–4816. Print.
@article{184742,
  abstract     = {Hydrogen peroxide (H2O2) has been implicated in many stress conditions, Control of H2O2 levels is complex and dissection of mechanisms generating and relieving H2O2 stress is difficult, particularly in intact plants, We have used transgenic tobacco with similar to 10\% wild-type catalase activity to study the role of catalase and effects of H2O2 stress in plants, Catalase-deficient plants showed no visible disorders at low Light, but in elevated light rapidly developed white necrotic lesions on the leaves, Lesion formation required photorespiratory activity since damage was prevented under elevated CO2, Accumulation of H2O2 was not detected during leaf necrosis, Alternative H2O2-scavenging mechanisms may have compensated for reduced catalase activity, as shown by increased ascorbate peroxidase and glutathione peroxidase levels, Leaf necrosis correlated with accumulation of oxidized glutathione and a 4-fold decrease in ascorbate, indicating that catalase is critical for maintaining the redox balance during oxidative stress, Such control may not be limited to peroxisomal H2O2 production, Catalase functions as a cellular sink for H2O2, as evidenced by complementation of catalase deficiency by exogenous catalase, and comparison of catalase-deficient and control leaf discs in removing external H2O2. Stress analysis revealed increased susceptibility-of catalase-deficient plants to paraquat, salt and ozone, but not to chilling.},
  author       = {Willekens, Hilde and Chamnongpol, Sangpen and Davey, Mark W and Schraudner, Martina and Langebartels, Christian and Van Montagu, Marc and Inz{\'e}, Dirk and Van Camp, Wim},
  issn         = {0261-4189},
  journal      = {EMBO JOURNAL},
  keyword      = {ASCORBATE PEROXIDASE,GLUTATHIONE-REDUCTASE,SIGNAL-TRANSDUCTION,DEFICIENT MUTANT,HYDROGEN-PEROXIDE,TRANSGENIC TOBACCO,ascorbate-glutathione cycle,ozone stress,photorespiration,redox control,salt stress,DISEASE RESISTANCE RESPONSE,HORDEUM-VULGARE-L,OXIDATIVE STRESS,SUPEROXIDE-DISMUTASE},
  language     = {eng},
  number       = {16},
  pages        = {4806--4816},
  title        = {Catalase is a sink for H\unmatched{2082}O\unmatched{2082} and is indispensable for stress defence in C\unmatched{2083} plants},
  url          = {http://dx.doi.org/10.1093/emboj/16.16.4806},
  volume       = {16},
  year         = {1997},
}

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