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Expression of a novel-type small proline-rich protein gene of alfalfa is induced by 2,4-dichlorophenoxiacetic acid in dedifferentiated callus cells

(1997) PLANT MOLECULAR BIOLOGY. 34(4). p.593-602
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Abstract
Differential screening of a cDNA library of 2,4-dichlorophenoxiacetic acid (2,4-D)-treated alfalfa (Medicago sativa) callus tissues resulted in the isolation of a 571 bp cDNA clone (MsPRP5) encoding for a proline-rich protein (84 amino acids) with a specific repeat unit of TPVLPPRK/(R)GRPPPVPP. In addition, a characteristic amino acid block (PPVYK) previously found in other proline-rich proteins also occurs in the C-terminal region of MsPRP5. At the N-terminal, a signal peptide similar to leader sequences of extracellular proteins can be predicted. According to the northern analysis, the corresponding gene is not expressed or is weakly expressed in differentiated vegetative organs and somatic embryos. However the accumulation of MsPRP5 mRNA is auxin concentration-dependent in dedifferentiated callus tissue. An increase in the amount of steady-state mRNA was detected already 20 min after auxin shock (100 mu M 2,4-D). Maximum expression was observed at 24-48 h in the presence of 2,4-D. Elevated expression was also found in cells recovering after heat shock and wounding stress. In synchronized alfalfa cells, the transcript level of MsPRP5 gene fluctuated during cell cycle progression with peaks in G1/S phase cells. Considering the structural features and expression properties of MsPRP5, this clone may represents a new type of proline-rich protein gene which responds to hormonal shock and some other stresses as well.
Keywords
CDNA, SITES, ACTIVATION, SEQUENCE, PATTERNS, PRODUCT, CARROT EXTENSIN, WALL PROTEINS, cell cycle, stress response, proline-rich protein, auxin response, cDNA, Medicago sativa, SOMATIC EMBRYOGENESIS, MEDICAGO-TRUNCATULA

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Citation

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Chicago
Györgyey, János, Kinga Németh, Zoltán Magyar, Zsolt Kelemen, Thierry Alliotte, Dirk Inzé, and Dénes Dudits. 1997. “Expression of a Novel-type Small Proline-rich Protein Gene of Alfalfa Is Induced by 2,4-dichlorophenoxiacetic Acid in Dedifferentiated Callus Cells.” Plant Molecular Biology 34 (4): 593–602.
APA
Györgyey, J., Németh, K., Magyar, Z., Kelemen, Z., Alliotte, T., Inzé, D., & Dudits, D. (1997). Expression of a novel-type small proline-rich protein gene of alfalfa is induced by 2,4-dichlorophenoxiacetic acid in dedifferentiated callus cells. PLANT MOLECULAR BIOLOGY, 34(4), 593–602.
Vancouver
1.
Györgyey J, Németh K, Magyar Z, Kelemen Z, Alliotte T, Inzé D, et al. Expression of a novel-type small proline-rich protein gene of alfalfa is induced by 2,4-dichlorophenoxiacetic acid in dedifferentiated callus cells. PLANT MOLECULAR BIOLOGY. 1997;34(4):593–602.
MLA
Györgyey, János, Kinga Németh, Zoltán Magyar, et al. “Expression of a Novel-type Small Proline-rich Protein Gene of Alfalfa Is Induced by 2,4-dichlorophenoxiacetic Acid in Dedifferentiated Callus Cells.” PLANT MOLECULAR BIOLOGY 34.4 (1997): 593–602. Print.
@article{184439,
  abstract     = {Differential screening of a cDNA library of 2,4-dichlorophenoxiacetic acid (2,4-D)-treated alfalfa (Medicago sativa) callus tissues resulted in the isolation of a 571 bp cDNA clone (MsPRP5) encoding for a proline-rich protein (84 amino acids) with a specific repeat unit of TPVLPPRK/(R)GRPPPVPP. In addition, a characteristic amino acid block (PPVYK) previously found in other proline-rich proteins also occurs in the C-terminal region of MsPRP5. At the N-terminal, a signal peptide similar to leader sequences of extracellular proteins can be predicted. According to the northern analysis, the corresponding gene is not expressed or is weakly expressed in differentiated vegetative organs and somatic embryos. However the accumulation of MsPRP5 mRNA is auxin concentration-dependent in dedifferentiated callus tissue. An increase in the amount of steady-state mRNA was detected already 20 min after auxin shock (100 mu M 2,4-D). Maximum expression was observed at 24-48 h in the presence of 2,4-D. Elevated expression was also found in cells recovering after heat shock and wounding stress. In synchronized alfalfa cells, the transcript level of MsPRP5 gene fluctuated during cell cycle progression with peaks in G1/S phase cells. Considering the structural features and expression properties of MsPRP5, this clone may represents a new type of proline-rich protein gene which responds to hormonal shock and some other stresses as well.},
  author       = {Györgyey, János and Németh, Kinga and Magyar, Zoltán and Kelemen, Zsolt and Alliotte, Thierry and Inzé, Dirk and Dudits, Dénes},
  issn         = {0167-4412},
  journal      = {PLANT MOLECULAR BIOLOGY},
  keywords     = {CDNA,SITES,ACTIVATION,SEQUENCE,PATTERNS,PRODUCT,CARROT EXTENSIN,WALL PROTEINS,cell cycle,stress response,proline-rich protein,auxin response,cDNA,Medicago sativa,SOMATIC EMBRYOGENESIS,MEDICAGO-TRUNCATULA},
  language     = {eng},
  number       = {4},
  pages        = {593--602},
  title        = {Expression of a novel-type small proline-rich protein gene of alfalfa is induced by 2,4-dichlorophenoxiacetic acid in dedifferentiated callus cells},
  url          = {http://dx.doi.org/10.1023/A:1005845412667},
  volume       = {34},
  year         = {1997},
}

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