Improvement of the maturation and germination of horse chestnut somatic embryos
- Author
- Maurizio Capuana and Pierre Debergh
- Organization
- Abstract
- Embryogenic tissues obtained from stamen filament cultures of horse chestnut (Aesculum hippocastanum L.) were cultured on maturation media supplemented with different combinations of abscisic acid, polyethylene glycol 4000, mannitol or activated charcoal. Somatic embryos were subjected to different desiccation procedures after a culture period on maturation media. After a slow desiccation, obtained by placing the somatic embryos in empty and non-sealed Petri dishes under the laminar air flow for 48 h, an increase in viability, shoot elongation and conversion was observed for the embryos previously cultured on medium enriched with ABA (80 mu M) alone or plus PEG (50 gl(-1)).
- Keywords
- Aesculus hippocastanum, abscisic acid, desiccation, polyethylene glycol, MICROSPORE-DERIVED EMBRYOS, AESCULUS-HIPPOCASTANUM L, BRASSICA-NAPUS L, ABSCISIC-ACID, DESICCATION TOLERANCE, PLANT-REGENERATION, STORAGE PROTEINS, PICEA-MARIANA, LEAF EXPLANTS, EMBRYOGENESIS
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Citation
Please use this url to cite or link to this publication: http://hdl.handle.net/1854/LU-183730
- MLA
- Capuana, Maurizio, and Pierre Debergh. “Improvement of the Maturation and Germination of Horse Chestnut Somatic Embryos.” PLANT CELL TISSUE AND ORGAN CULTURE, vol. 48, no. 1, 1997, pp. 23–29, doi:10.1023/A:1005890826431.
- APA
- Capuana, M., & Debergh, P. (1997). Improvement of the maturation and germination of horse chestnut somatic embryos. PLANT CELL TISSUE AND ORGAN CULTURE, 48(1), 23–29. https://doi.org/10.1023/A:1005890826431
- Chicago author-date
- Capuana, Maurizio, and Pierre Debergh. 1997. “Improvement of the Maturation and Germination of Horse Chestnut Somatic Embryos.” PLANT CELL TISSUE AND ORGAN CULTURE 48 (1): 23–29. https://doi.org/10.1023/A:1005890826431.
- Chicago author-date (all authors)
- Capuana, Maurizio, and Pierre Debergh. 1997. “Improvement of the Maturation and Germination of Horse Chestnut Somatic Embryos.” PLANT CELL TISSUE AND ORGAN CULTURE 48 (1): 23–29. doi:10.1023/A:1005890826431.
- Vancouver
- 1.Capuana M, Debergh P. Improvement of the maturation and germination of horse chestnut somatic embryos. PLANT CELL TISSUE AND ORGAN CULTURE. 1997;48(1):23–9.
- IEEE
- [1]M. Capuana and P. Debergh, “Improvement of the maturation and germination of horse chestnut somatic embryos,” PLANT CELL TISSUE AND ORGAN CULTURE, vol. 48, no. 1, pp. 23–29, 1997.
@article{183730,
abstract = {{Embryogenic tissues obtained from stamen filament cultures of horse chestnut (Aesculum hippocastanum L.) were cultured on maturation media supplemented with different combinations of abscisic acid, polyethylene glycol 4000, mannitol or activated charcoal. Somatic embryos were subjected to different desiccation procedures after a culture period on maturation media. After a slow desiccation, obtained by placing the somatic embryos in empty and non-sealed Petri dishes under the laminar air flow for 48 h, an increase in viability, shoot elongation and conversion was observed for the embryos previously cultured on medium enriched with ABA (80 mu M) alone or plus PEG (50 gl(-1)).}},
author = {{Capuana, Maurizio and Debergh, Pierre}},
issn = {{0167-6857}},
journal = {{PLANT CELL TISSUE AND ORGAN CULTURE}},
keywords = {{Aesculus hippocastanum,abscisic acid,desiccation,polyethylene glycol,MICROSPORE-DERIVED EMBRYOS,AESCULUS-HIPPOCASTANUM L,BRASSICA-NAPUS L,ABSCISIC-ACID,DESICCATION TOLERANCE,PLANT-REGENERATION,STORAGE PROTEINS,PICEA-MARIANA,LEAF EXPLANTS,EMBRYOGENESIS}},
language = {{eng}},
number = {{1}},
pages = {{23--29}},
title = {{Improvement of the maturation and germination of horse chestnut somatic embryos}},
url = {{http://doi.org/10.1023/A:1005890826431}},
volume = {{48}},
year = {{1997}},
}
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