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Characterization of avian Chlamydia psittaci strains using omp1 restriction mapping and serovar-specific monoclonal antibodies

(1997) RESEARCH IN MICROBIOLOGY. 148(4). p.327-333
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Abstract
In the present study, 60 avian Chlamydia psittaci isolates were characterized using restriction fragment length polymorphism as well as serovar-specific monoclonal antibodies, enabling a comparison between the two characterization methods. Sixty avian C. psittaci isolates were characterized by AluI restriction mapping of the major outer membrane protein gene omp1 obtained after amplification by the polymerase chain reaction. The 60 avian C. psittaci strains were also characterized using serovar-specific monoclonal antibodies in a microimmunofluorescence test. Digestion of 60 avian C. psittaci omp1 amplicons by AluI generated 5 of the 6 known distinct restriction patterns (A, B, D, E and F). Restriction pattern C was not observed. Serotyping revealed 4 avian C. psittaci serovars (A, B, C and D). None of the 60 isolates was typed as serovar E. AluI restriction patterns A, B, D and E corresponded in 98% of the cases to serovars A, B, C and D, respectively. One isolate, classified as serovar A, generated restriction pattern F instead of A. Genotyping enabled a more precise differentiation of avian C. psittaci serovar A strains. Serovar A strains were divided into two groups according to their AluI restriction pattern (A or F). For epidemiological studies, genotyping can thus be a highly valuable alternative to serotyping, especially when applied directly to the clinical samples.
Keywords
avian strains, Chlamydia psittaci, serotyping, omp1, restriction mapping, OUTER-MEMBRANE PROTEIN, POLYMERASE CHAIN-REACTION, DIFFERENTIATION, NUCLEOTIDE, DIVERSITY, GENE

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Chicago
Vanrompay, Daisy, Patrick Butaye, C Sayada, Richard Ducatelle, and Freddy Haesebrouck. 1997. “Characterization of Avian Chlamydia Psittaci Strains Using Omp1 Restriction Mapping and Serovar-specific Monoclonal Antibodies.” Research in Microbiology 148 (4): 327–333.
APA
Vanrompay, D., Butaye, P., Sayada, C., Ducatelle, R., & Haesebrouck, F. (1997). Characterization of avian Chlamydia psittaci strains using omp1 restriction mapping and serovar-specific monoclonal antibodies. RESEARCH IN MICROBIOLOGY, 148(4), 327–333.
Vancouver
1.
Vanrompay D, Butaye P, Sayada C, Ducatelle R, Haesebrouck F. Characterization of avian Chlamydia psittaci strains using omp1 restriction mapping and serovar-specific monoclonal antibodies. RESEARCH IN MICROBIOLOGY. 1997;148(4):327–33.
MLA
Vanrompay, Daisy, Patrick Butaye, C Sayada, et al. “Characterization of Avian Chlamydia Psittaci Strains Using Omp1 Restriction Mapping and Serovar-specific Monoclonal Antibodies.” RESEARCH IN MICROBIOLOGY 148.4 (1997): 327–333. Print.
@article{183413,
  abstract     = {In the present study, 60 avian Chlamydia psittaci isolates were characterized using restriction fragment length polymorphism as well as serovar-specific monoclonal antibodies, enabling a comparison between the two characterization methods. Sixty avian C. psittaci isolates were characterized by AluI restriction mapping of the major outer membrane protein gene omp1 obtained after amplification by the polymerase chain reaction. The 60 avian C. psittaci strains were also characterized using serovar-specific monoclonal antibodies in a microimmunofluorescence test.
Digestion of 60 avian C. psittaci omp1 amplicons by AluI generated 5 of the 6 known distinct restriction patterns (A, B, D, E and F). Restriction pattern C was not observed. Serotyping revealed 4 avian C. psittaci serovars (A, B, C and D). None of the 60 isolates was typed as serovar E. AluI restriction patterns A, B, D and E corresponded in 98% of the cases to serovars A, B, C and D, respectively. One isolate, classified as serovar A, generated restriction pattern F instead of A. Genotyping enabled a more precise differentiation of avian C. psittaci serovar A strains. Serovar A strains were divided into two groups according to their AluI restriction pattern (A or F). For epidemiological studies, genotyping can thus be a highly valuable alternative to serotyping, especially when applied directly to the clinical samples.},
  author       = {Vanrompay, Daisy and Butaye, Patrick and Sayada, C and Ducatelle, Richard and Haesebrouck, Freddy},
  issn         = {0923-2508},
  journal      = {RESEARCH IN MICROBIOLOGY},
  keywords     = {avian strains,Chlamydia psittaci,serotyping,omp1,restriction mapping,OUTER-MEMBRANE PROTEIN,POLYMERASE CHAIN-REACTION,DIFFERENTIATION,NUCLEOTIDE,DIVERSITY,GENE},
  language     = {eng},
  number       = {4},
  pages        = {327--333},
  title        = {Characterization of avian Chlamydia psittaci strains using omp1 restriction mapping and serovar-specific monoclonal antibodies},
  url          = {http://dx.doi.org/10.1016/S0923-2508(97)81588-4},
  volume       = {148},
  year         = {1997},
}

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