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Use of metal chelate affinity chromatography and membrane-based ion-exchange as clean-up procedure for trace residue analysis of tetracyclines in animal tissues and egg

Siska Croubels UGent, Kristina Vanoosthuyze and Carlos Van Peteghem (1997) JOURNAL OF CHROMATOGRAPHY B. 690(1-2). p.173-179
abstract
A new and efficient procedure for the clean-up of tetracycline residues in animal tissues and egg prior to reversed-phase high-performance liquid chromatography is described. The principal steps involve homogenization of the tissues in sodium succinate buffer and methanol, followed by centrifugation and clean-up with metal chelate affinity chromatography (MCAC). After further concentration on an Empore extraction membrane with cation-exchange properties, the sample is analysed by HPLC with fluorescence detection. The method was tested on porcine kidney and muscle, bovine liver and whole chicken's egg. The recoveries were determined from spiked tissues for oxytetracycline, tetracycline, chlortetracycline and doxycycline and ranged from 40 to 70%, with repeatabilities below 10% R.S.D.. The analytical responses were linear in the range up to at least 1000 ng/g. The detection limits of the method were estimated at 0.42 ng/g of oxytetracycline, 0.49 ng/g of tetracycline, 0.66 ng/g of chlortetracycline and 1.38 ng/g of doxycycline in porcine muscle, using signal-to-noise ratios of 4:1. Similar detection limits were estimated for kidney, liver and el:g. The measured limits of quantification were 2 ng/g for oxytetracycline, 3 ng/g for tetracycline, 4 ng/g for chlortetracycline and 5 ng/g for doxycycline in porcine kidney. The advantage of this method over existing methods is its increased limit of detection.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
MILK, HPLC, oxytetracycline, tetracycline, chlortetracycline, doxycycline, PERFORMANCE LIQUID-CHROMATOGRAPHY, ANTIBIOTICS, EXTRACTION, BOVINE, DISKS, URINE
journal title
JOURNAL OF CHROMATOGRAPHY B
J. Chromatogr. B
volume
690
issue
1-2
pages
173 - 179
Web of Science type
Article
ISSN
0378-4347
DOI
10.1016/S0378-4347(96)00368-4
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
183023
handle
http://hdl.handle.net/1854/LU-183023
date created
2004-01-14 13:41:00
date last changed
2016-12-19 15:39:12
@article{183023,
  abstract     = {A new and efficient procedure for the clean-up of tetracycline residues in animal tissues and egg prior to reversed-phase high-performance liquid chromatography is described. The principal steps involve homogenization of the tissues in sodium succinate buffer and methanol, followed by centrifugation and clean-up with metal chelate affinity chromatography (MCAC). After further concentration on an Empore extraction membrane with cation-exchange properties, the sample is analysed by HPLC with fluorescence detection. The method was tested on porcine kidney and muscle, bovine liver and whole chicken's egg. The recoveries were determined from spiked tissues for oxytetracycline, tetracycline, chlortetracycline and doxycycline and ranged from 40 to 70\%, with repeatabilities below 10\% R.S.D.. The analytical responses were linear in the range up to at least 1000 ng/g. The detection limits of the method were estimated at 0.42 ng/g of oxytetracycline, 0.49 ng/g of tetracycline, 0.66 ng/g of chlortetracycline and 1.38 ng/g of doxycycline in porcine muscle, using signal-to-noise ratios of 4:1. Similar detection limits were estimated for kidney, liver and el:g. The measured limits of quantification were 2 ng/g for oxytetracycline, 3 ng/g for tetracycline, 4 ng/g for chlortetracycline and 5 ng/g for doxycycline in porcine kidney. The advantage of this method over existing methods is its increased limit of detection.},
  author       = {Croubels, Siska and Vanoosthuyze, Kristina and Van Peteghem, Carlos},
  issn         = {0378-4347},
  journal      = {JOURNAL OF CHROMATOGRAPHY B},
  keyword      = {MILK,HPLC,oxytetracycline,tetracycline,chlortetracycline,doxycycline,PERFORMANCE LIQUID-CHROMATOGRAPHY,ANTIBIOTICS,EXTRACTION,BOVINE,DISKS,URINE},
  language     = {eng},
  number       = {1-2},
  pages        = {173--179},
  title        = {Use of metal chelate affinity chromatography and membrane-based ion-exchange as clean-up procedure for trace residue analysis of tetracyclines in animal tissues and egg},
  url          = {http://dx.doi.org/10.1016/S0378-4347(96)00368-4},
  volume       = {690},
  year         = {1997},
}

Chicago
Croubels, Siska, Kristina Vanoosthuyze, and Carlos Van Peteghem. 1997. “Use of Metal Chelate Affinity Chromatography and Membrane-based Ion-exchange as Clean-up Procedure for Trace Residue Analysis of Tetracyclines in Animal Tissues and Egg.” Journal of Chromatography B 690 (1-2): 173–179.
APA
Croubels, S., Vanoosthuyze, K., & Van Peteghem, C. (1997). Use of metal chelate affinity chromatography and membrane-based ion-exchange as clean-up procedure for trace residue analysis of tetracyclines in animal tissues and egg. JOURNAL OF CHROMATOGRAPHY B, 690(1-2), 173–179.
Vancouver
1.
Croubels S, Vanoosthuyze K, Van Peteghem C. Use of metal chelate affinity chromatography and membrane-based ion-exchange as clean-up procedure for trace residue analysis of tetracyclines in animal tissues and egg. JOURNAL OF CHROMATOGRAPHY B. 1997;690(1-2):173–9.
MLA
Croubels, Siska, Kristina Vanoosthuyze, and Carlos Van Peteghem. “Use of Metal Chelate Affinity Chromatography and Membrane-based Ion-exchange as Clean-up Procedure for Trace Residue Analysis of Tetracyclines in Animal Tissues and Egg.” JOURNAL OF CHROMATOGRAPHY B 690.1-2 (1997): 173–179. Print.