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Cell proliferation and hair tip growth in the Arabidopsis root are under mechanistically different forms of redox control

Rocío Sánchez-Fernández, Mark Fricker, Liz B Corben, Nick S White, Nicki Sheard, Christopher J Leaver, Marc Van Montagu UGent, Dirk Inzé UGent and Mike May UGent (1997) PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA. 94(6). p.2745-2750
abstract
We provide evidence that the tripeptide thiol glutathione (GSH) participates in the regulation of cell division in the apical meristem of Arabidopsis roots. Exogenous application of micromolar concentrations of GSH raised the number of meristematic cells undergoing mitosis, while depletion of GSH had the opposite effect, A role for endogenous GSH in the control of cell proliferation is also provided by mapping of GSH levels in the root meristem using the GSH-specific dye monochlorobimane and confocal laser scanning microscopy. High levels of GSH were associated with the epidermal and cortical initials and markedly lower levels in the quiescent center. The mechanisms controlling cell division could also be triggered by other reducing agents: ascorbic acid and dithiothreitol. Our data also reveal significant plasticity in the relationship between the trichoblast cell length and the hair it subtends in response to alterations in intracellular redox homeostasis. While mechanisms that control trichoblast elongation are influenced by nonspecific redox couples, root hair tip growth has a more specific requirement for sulfhydryl groups, The responses we describe here may represent the extremes of redox control of root plasticity and would allow the root to maintain exploration of the soil under adverse conditions with minimal cell divisions and root hair production or capitalize on a favorable environment by production of numerous long hairs. Redox sensing of the environment and subsequent redox-dependent modulation of growth and development may be crucial components in the strategies plants have evolved for survival in a fluctuating environment.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
ascorbate, ACTIVATION, bimane, confocal laser scanning microscopy, glutathione, thiols, GLUTATHIONE SYNTHESIS, MONOCHLOROBIMANE, ORGANIZATION, INITIATION
journal title
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
Proc. Natl. Acad. Sci. USA
volume
94
issue
6
pages
2745 - 2750
Web of Science type
Article
ISSN
0027-8424
DOI
10.1073/pnas.94.6.2745
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
182479
handle
http://hdl.handle.net/1854/LU-182479
date created
2004-01-14 13:41:00
date last changed
2016-12-19 15:38:23
@article{182479,
  abstract     = {We provide evidence that the tripeptide thiol glutathione (GSH) participates in the regulation of cell division in the apical meristem of Arabidopsis roots. Exogenous application of micromolar concentrations of GSH raised the number of meristematic cells undergoing mitosis, while depletion of GSH had the opposite effect, A role for endogenous GSH in the control of cell proliferation is also provided by mapping of GSH levels in the root meristem using the GSH-specific dye monochlorobimane and confocal laser scanning microscopy. High levels of GSH were associated with the epidermal and cortical initials and markedly lower levels in the quiescent center. The mechanisms controlling cell division could also be triggered by other reducing agents: ascorbic acid and dithiothreitol. Our data also reveal significant plasticity in the relationship between the trichoblast cell length and the hair it subtends in response to alterations in intracellular redox homeostasis. While mechanisms that control trichoblast elongation are influenced by nonspecific redox couples, root hair tip growth has a more specific requirement for sulfhydryl groups, The responses we describe here may represent the extremes of redox control of root plasticity and would allow the root to maintain exploration of the soil under adverse conditions with minimal cell divisions and root hair production or capitalize on a favorable environment by production of numerous long hairs. Redox sensing of the environment and subsequent redox-dependent modulation of growth and development may be crucial components in the strategies plants have evolved for survival in a fluctuating environment.},
  author       = {S{\'a}nchez-Fern{\'a}ndez, Roc{\'i}o and Fricker, Mark and Corben, Liz B and White, Nick S and Sheard, Nicki and Leaver, Christopher J and Van Montagu, Marc and Inz{\'e}, Dirk and May, Mike},
  issn         = {0027-8424},
  journal      = {PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA},
  keyword      = {ascorbate,ACTIVATION,bimane,confocal laser scanning microscopy,glutathione,thiols,GLUTATHIONE SYNTHESIS,MONOCHLOROBIMANE,ORGANIZATION,INITIATION},
  language     = {eng},
  number       = {6},
  pages        = {2745--2750},
  title        = {Cell proliferation and hair tip growth in the Arabidopsis root are under mechanistically different forms of redox control},
  url          = {http://dx.doi.org/10.1073/pnas.94.6.2745},
  volume       = {94},
  year         = {1997},
}

Chicago
Sánchez-Fernández, Rocío, Mark Fricker, Liz B Corben, Nick S White, Nicki Sheard, Christopher J Leaver, Marc Van Montagu, Dirk Inzé, and Mike May. 1997. “Cell Proliferation and Hair Tip Growth in the Arabidopsis Root Are Under Mechanistically Different Forms of Redox Control.” Proceedings of the National Academy of Sciences of the United States of America 94 (6): 2745–2750.
APA
Sánchez-Fernández, R., Fricker, M., Corben, L. B., White, N. S., Sheard, N., Leaver, C. J., Van Montagu, M., et al. (1997). Cell proliferation and hair tip growth in the Arabidopsis root are under mechanistically different forms of redox control. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 94(6), 2745–2750.
Vancouver
1.
Sánchez-Fernández R, Fricker M, Corben LB, White NS, Sheard N, Leaver CJ, et al. Cell proliferation and hair tip growth in the Arabidopsis root are under mechanistically different forms of redox control. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA. 1997;94(6):2745–50.
MLA
Sánchez-Fernández, Rocío, Mark Fricker, Liz B Corben, et al. “Cell Proliferation and Hair Tip Growth in the Arabidopsis Root Are Under Mechanistically Different Forms of Redox Control.” PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 94.6 (1997): 2745–2750. Print.