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G2-and early-M-specific expression of the NTCYC1 cyclin gene in Nicotiana tabacum cells

(1996) PLANT MOLECULAR BIOLOGY. 32(6). p.1093-1101
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Abstract
We have previously reported the isolation of a cDNA encoding a mitotic cyclin, NTCYC1, from a tobacco cell suspension library. Here we describe the expression patterns of NTCYC1 and pf Ntsuc1; a suc1 plant homologue, in synchronized tobacco cell suspensions. Furthermore, the expression pattern of this cyclin is compared to that of Ntcdc2-1, a Nicotiana tabacum homologue of cdc2. While no NTCYC1 transcript was detected in cells synchronized in the G1 and S phases, NTCYC1 expression was observed in late G2 and early M phases, disappearing in the G1' of a new cell cycle. On the other hand, Ntsuc1 and Ntcdc2-1 exhibited a constitutive expression during the cell cycle. A functional analysis performed by microinjecting NTCYC1 mRNA into immature Xenopus oocytes, indicates that NTCYC1 could participate in the control of the G2/M transition in plant cells. Subsequently NTCYC1 expression was used to assess the status of mesophyll cells in expanded leaves of N. tabacum. Depending on leaf position along the shoot axis, a large population of mesophyll cells appeared with a 4C DNA content, suggesting a G2 arrest. It was found that leaves with such a population also contained high levels of NTCYC1 transcripts. With respect to these results concerning a naturally occurring G2-arrested cell population, the regulation of NTCYC1 expression in planta is discussed.
Keywords
gene expression, cell division cycle, mitotic cyclin, plant suc1 homologue, Ntcdc2-1, Nicotiana tabacum, SCHIZOSACCHAROMYCES-POMBE, SACCHAROMYCES-CEREVISIAE, ARABIDOPSIS-THALIANA, G2-M TRANSITION, MITOTIC CYCLINS, MESSENGER-RNA, FISSION YEAST, HIGHER-PLANTS, A-TYPE, PROTEIN

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Citation

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Chicago
Qin, Li-Xian, Claudette Perennes, Luc Richard, Michelle Bouvier Durand, Christophe Tréhin, Dirk Inzé, and Catherine Bergounioux. 1996. “G2-and early-M-specific Expression of the NTCYC1 Cyclin Gene in Nicotiana Tabacum Cells.” Plant Molecular Biology 32 (6): 1093–1101.
APA
Qin, L.-X., Perennes, C., Richard, L., Bouvier Durand, M., Tréhin, C., Inzé, D., & Bergounioux, C. (1996). G2-and early-M-specific expression of the NTCYC1 cyclin gene in Nicotiana tabacum cells. PLANT MOLECULAR BIOLOGY, 32(6), 1093–1101.
Vancouver
1.
Qin L-X, Perennes C, Richard L, Bouvier Durand M, Tréhin C, Inzé D, et al. G2-and early-M-specific expression of the NTCYC1 cyclin gene in Nicotiana tabacum cells. PLANT MOLECULAR BIOLOGY. 1996;32(6):1093–101.
MLA
Qin, Li-Xian, Claudette Perennes, Luc Richard, et al. “G2-and early-M-specific Expression of the NTCYC1 Cyclin Gene in Nicotiana Tabacum Cells.” PLANT MOLECULAR BIOLOGY 32.6 (1996): 1093–1101. Print.
@article{181723,
  abstract     = {We have previously reported the isolation of a cDNA encoding a mitotic cyclin, NTCYC1, from a tobacco cell suspension library. Here we describe the expression patterns of NTCYC1 and pf Ntsuc1; a suc1 plant homologue, in synchronized tobacco cell suspensions. Furthermore, the expression pattern of this cyclin is compared to that of Ntcdc2-1, a Nicotiana tabacum homologue of cdc2. While no NTCYC1 transcript was detected in cells synchronized in the G1 and S phases, NTCYC1 expression was observed in late G2 and early M phases, disappearing in the G1' of a new cell cycle. On the other hand, Ntsuc1 and Ntcdc2-1 exhibited a constitutive expression during the cell cycle. A functional analysis performed by microinjecting NTCYC1 mRNA into immature Xenopus oocytes, indicates that NTCYC1 could participate in the control of the G2/M transition in plant cells. Subsequently NTCYC1 expression was used to assess the status of mesophyll cells in expanded leaves of N. tabacum. Depending on leaf position along the shoot axis, a large population of mesophyll cells appeared with a 4C DNA content, suggesting a G2 arrest. It was found that leaves with such a population also contained high levels of NTCYC1 transcripts. With respect to these results concerning a naturally occurring G2-arrested cell population, the regulation of NTCYC1 expression in planta is discussed.},
  author       = {Qin, Li-Xian and Perennes, Claudette and Richard, Luc and Bouvier Durand, Michelle and Tr{\'e}hin, Christophe and Inz{\'e}, Dirk and Bergounioux, Catherine},
  issn         = {0167-4412},
  journal      = {PLANT MOLECULAR BIOLOGY},
  keyword      = {gene expression,cell division cycle,mitotic cyclin,plant suc1 homologue,Ntcdc2-1,Nicotiana tabacum,SCHIZOSACCHAROMYCES-POMBE,SACCHAROMYCES-CEREVISIAE,ARABIDOPSIS-THALIANA,G2-M TRANSITION,MITOTIC CYCLINS,MESSENGER-RNA,FISSION YEAST,HIGHER-PLANTS,A-TYPE,PROTEIN},
  language     = {eng},
  number       = {6},
  pages        = {1093--1101},
  title        = {G2-and early-M-specific expression of the NTCYC1 cyclin gene in Nicotiana tabacum cells},
  url          = {http://dx.doi.org/10.1007/BF00041393},
  volume       = {32},
  year         = {1996},
}

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