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Factors influencing Agrobacterium tumefaciens-mediated transformation of Artemisia annua L.

Annemieke Vergauwe UGent, Els Van Geldre UGent, Dirk Inzé UGent, Marc Van Montagu UGent and Elfride Van den Eeckhout UGent (1998) PLANT CELL REPORTS. 18(1-2). p.105-110
abstract
Following our previously described Agrobacterium tumefaciens-mediated transformation procedure for Artemisia annua L., we have undertaken several additional experiments to establish the importance of some parameters such as explant type, age of explant source, A. tumefaciens strain and type of binary vector. Several binary vectors were useful for the production of transgenic callus on explants of different ages. In transformed calli, a good correlation between integration and expression of foreign DNA was observed: different assays showed expression of beta-Glucuronidase, neomycin phosphotransferase II, superoxide dismutase and bleomycin acetyl transferase. The regeneration of transgenic plants required more restricted conditions. Only with the pTJK136 Vector could transgenic plants be obtained from leaf and stem explants from 12- to 18-week-old plants. Go-cultivation for 48 h seemed favorable for the regeneration of transgenic plants. Stable integration and expression of the transgenes was also shown in the progeny.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
Agrobacterium tumefaciens, antimalarial drugs, Artemisia annua L., transformation, MANGANESE SUPEROXIDE-DISMUTASE, TRANSGENIC PLANTS, DNA, EXPRESSION, PROMOTER, PLASMID, GENES
journal title
PLANT CELL REPORTS
Plant Cell Reports
volume
18
issue
1-2
pages
105 - 110
Web of Science type
Article
Web of Science id
000077189900019
ISSN
0721-7714
DOI
10.1007/s002990050540
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
180931
handle
http://hdl.handle.net/1854/LU-180931
date created
2004-01-14 13:41:00
date last changed
2013-10-24 14:21:56
@article{180931,
  abstract     = {Following our previously described Agrobacterium tumefaciens-mediated transformation procedure for Artemisia annua L., we have undertaken several additional experiments to establish the importance of some parameters such as explant type, age of explant source, A. tumefaciens strain and type of binary vector. Several binary vectors were useful for the production of transgenic callus on explants of different ages. In transformed calli, a good correlation between integration and expression of foreign DNA was observed: different assays showed expression of beta-Glucuronidase, neomycin phosphotransferase II, superoxide dismutase and bleomycin acetyl transferase. The regeneration of transgenic plants required more restricted conditions. Only with the pTJK136 Vector could transgenic plants be obtained from leaf and stem explants from 12- to 18-week-old plants. Go-cultivation for 48 h seemed favorable for the regeneration of transgenic plants. Stable integration and expression of the transgenes was also shown in the progeny.},
  author       = {Vergauwe, Annemieke and Van Geldre, Els and Inz{\'e}, Dirk and Van Montagu, Marc and Van den Eeckhout, Elfride},
  issn         = {0721-7714},
  journal      = {PLANT CELL REPORTS},
  keyword      = {Agrobacterium tumefaciens,antimalarial drugs,Artemisia annua L.,transformation,MANGANESE SUPEROXIDE-DISMUTASE,TRANSGENIC PLANTS,DNA,EXPRESSION,PROMOTER,PLASMID,GENES},
  language     = {eng},
  number       = {1-2},
  pages        = {105--110},
  title        = {Factors influencing Agrobacterium tumefaciens-mediated transformation of Artemisia annua L.},
  url          = {http://dx.doi.org/10.1007/s002990050540},
  volume       = {18},
  year         = {1998},
}

Chicago
Vergauwe, Annemieke, Els Van Geldre, Dirk Inzé, Marc Van Montagu, and Elfride Van den Eeckhout. 1998. “Factors Influencing Agrobacterium Tumefaciens-mediated Transformation of Artemisia Annua L.” Plant Cell Reports 18 (1-2): 105–110.
APA
Vergauwe, A., Van Geldre, E., Inzé, D., Van Montagu, M., & Van den Eeckhout, E. (1998). Factors influencing Agrobacterium tumefaciens-mediated transformation of Artemisia annua L. PLANT CELL REPORTS, 18(1-2), 105–110.
Vancouver
1.
Vergauwe A, Van Geldre E, Inzé D, Van Montagu M, Van den Eeckhout E. Factors influencing Agrobacterium tumefaciens-mediated transformation of Artemisia annua L. PLANT CELL REPORTS. 1998;18(1-2):105–10.
MLA
Vergauwe, Annemieke, Els Van Geldre, Dirk Inzé, et al. “Factors Influencing Agrobacterium Tumefaciens-mediated Transformation of Artemisia Annua L.” PLANT CELL REPORTS 18.1-2 (1998): 105–110. Print.