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Altered expression of ocEß7 integrin on intra-epithelial and lamina propria lymphocytes in patients with Crohn's disease

Dirk Elewaut UGent, Nancy Van Damme UGent, Filip De Keyser UGent, Dominique Baeten UGent, Pascale De Paepe, Hans Van Vlierberghe UGent, Herman Mielants, Claude Cuvelier, August Verbruggen UGent, Eric Veys, et al. (1998) ACTA GASTRO-ENTEROLOGICA BELGICA. 61(3). p.288-294
abstract
Objective: To compare the expression of adhesion molecules on intestinal intra-epithelial (IEL) and lamina propria T cells (LPL) from ileum and colon, in patients with Crohn's disease (CD) versus healthy controls, with special reference for the alpha E beta 7 integrin. Methods: IEL and LPL were obtained from 18 CD patients and 20 controls by enzymatic extraction, and subsequently characterized by now cytometry for CD3, CD4, CD8, CD25, LFA-1 alpha (CD11a), CD44, alpha 4 and alpha E beta 7 integrin. Results: In LPL of controls, a decreased CD4/CD8 ratio was noted in ileum compared to colon. This regional difference was accompanied by a higher expression of alpha E beta 7 integrin in ileum versus colon. In LPL from left hemicolon of CD patients, a decreased CD4/CD8 ratio was noted versus controls. alpha E beta 7 expression on T cells of LPL did not discriminate CD from controls. However, an overexpression of this beta 7 integrin member was observed on CD25+ T cell subsets from lamina propria of left hemicolon, in CD versus controls. Moreover, in IEL, profound alterations in alpha E beta 7 integrin were observed in CD, compared to controls. A decreased expression of alpha E beta 7 was noted in IEL of ileum of CD patients. This was also apparent in non-inflamed mucosa. Conclusion: The observed changes of alpha E beta 7 integrin expression in CD patients versus controls are of pathogenic relevance, especially the decreased expression of alpha e beta 7 in IEL of non-inflamed CD mucosa. This may be one of the earliest events in the pathogenesis of this disease.
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author
organization
alternative title
Altered expression of alpha E beta 7 integrin on intra-epithelial and lamina propria lymphocytes in patients with Crohn's disease
year
type
journalArticle (original)
publication status
published
subject
keyword
INTESTINAL INTRAEPITHELIAL LYMPHOCYTES, INFLAMMATORY BOWEL-DISEASE, Crohn's disease, MONOCLONAL-ANTIBODY, T-CELLS, ULCERATIVE-COLITIS, COTTON-TOP TAMARIN, FLOW CYTOMETRIC ANALYSIS, adhesion molecules, alpha E beta 7 integrin, HUMAN GUT, PERIPHERAL-BLOOD, IMMUNE-SYSTEM
journal title
ACTA GASTRO-ENTEROLOGICA BELGICA
Acta Gastro-Enterol. Belg.
volume
61
issue
3
pages
288 - 294
Web of Science type
Article
Web of Science id
000076536300003
ISSN
0001-5644
language
English
UGent publication?
yes
classification
A1
id
180236
handle
http://hdl.handle.net/1854/LU-180236
date created
2004-01-14 13:41:00
date last changed
2016-12-19 15:46:09
@article{180236,
  abstract     = {Objective: To compare the expression of adhesion molecules on intestinal intra-epithelial (IEL) and lamina propria T cells (LPL) from ileum and colon, in patients with Crohn's disease (CD) versus healthy controls, with special reference for the alpha E beta 7 integrin. 
Methods: IEL and LPL were obtained from 18 CD patients and 20 controls by enzymatic extraction, and subsequently characterized by now cytometry for CD3, CD4, CD8, CD25, LFA-1 alpha (CD11a), CD44, alpha 4 and alpha E beta 7 integrin. 
Results: In LPL of controls, a decreased CD4/CD8 ratio was noted in ileum compared to colon. This regional difference was accompanied by a higher expression of alpha E beta 7 integrin in ileum versus colon. In LPL from left hemicolon of CD patients, a decreased CD4/CD8 ratio was noted versus controls. alpha E beta 7 expression on T cells of LPL did not discriminate CD from controls. However, an overexpression of this beta 7 integrin member was observed on CD25+ T cell subsets from lamina propria of left hemicolon, in CD versus controls. 
Moreover, in IEL, profound alterations in alpha E beta 7 integrin were observed in CD, compared to controls. A decreased expression of alpha E beta 7 was noted in IEL of ileum of CD patients. This was also apparent in non-inflamed mucosa. 
Conclusion: The observed changes of alpha E beta 7 integrin expression in CD patients versus controls are of pathogenic relevance, especially the decreased expression of alpha e beta 7 in IEL of non-inflamed CD mucosa. This may be one of the earliest events in the pathogenesis of this disease.},
  author       = {Elewaut, Dirk and Van Damme, Nancy and De Keyser, Filip and Baeten, Dominique and De Paepe, Pascale and Van Vlierberghe, Hans and Mielants, Herman and Cuvelier, Claude and Verbruggen, August and Veys, Eric and De Vos, Martine},
  issn         = {0001-5644},
  journal      = {ACTA GASTRO-ENTEROLOGICA BELGICA},
  keyword      = {INTESTINAL INTRAEPITHELIAL LYMPHOCYTES,INFLAMMATORY BOWEL-DISEASE,Crohn's disease,MONOCLONAL-ANTIBODY,T-CELLS,ULCERATIVE-COLITIS,COTTON-TOP TAMARIN,FLOW CYTOMETRIC ANALYSIS,adhesion molecules,alpha E beta 7 integrin,HUMAN GUT,PERIPHERAL-BLOOD,IMMUNE-SYSTEM},
  language     = {eng},
  number       = {3},
  pages        = {288--294},
  title        = {Altered expression of ocE{\ss}7 integrin on intra-epithelial and lamina propria lymphocytes in patients with Crohn's disease},
  volume       = {61},
  year         = {1998},
}

Chicago
Elewaut, Dirk, Nancy Van Damme, Filip De Keyser, Dominique Baeten, Pascale De Paepe, Hans Van Vlierberghe, Herman Mielants, et al. 1998. “Altered Expression of ocEß7 Integrin on Intra-epithelial and Lamina Propria Lymphocytes in Patients with Crohn’s Disease.” Acta Gastro-enterologica Belgica 61 (3): 288–294.
APA
Elewaut, D., Van Damme, N., De Keyser, F., Baeten, D., De Paepe, P., Van Vlierberghe, H., Mielants, H., et al. (1998). Altered expression of ocEß7 integrin on intra-epithelial and lamina propria lymphocytes in patients with Crohn’s disease. ACTA GASTRO-ENTEROLOGICA BELGICA, 61(3), 288–294.
Vancouver
1.
Elewaut D, Van Damme N, De Keyser F, Baeten D, De Paepe P, Van Vlierberghe H, et al. Altered expression of ocEß7 integrin on intra-epithelial and lamina propria lymphocytes in patients with Crohn’s disease. ACTA GASTRO-ENTEROLOGICA BELGICA. 1998;61(3):288–94.
MLA
Elewaut, Dirk, Nancy Van Damme, Filip De Keyser, et al. “Altered Expression of ocEß7 Integrin on Intra-epithelial and Lamina Propria Lymphocytes in Patients with Crohn’s Disease.” ACTA GASTRO-ENTEROLOGICA BELGICA 61.3 (1998): 288–294. Print.