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Recombinant porcine IFN-γ potentiates the secondary IgG and IgA responses to an inactivated suid herpesvirus-1 vaccine and reduces postchallenge weight loss and fever in pigs

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Abstract
The effect of recombinant porcine interferon-gamma (IFN-gamma) on the immunogenicity in vivo of inactivated suid herpesvirus-1 (SHV-1, Phylaxia strain) was studied applying two successive i.m, immunizations. The animals were injected with inactivated virus alone or inactivated virus supplemented with 10(4) or 10(6) U IFN-gamma. After the first immunization, none of the animals responded with measurable virus-neutralizing antibody (VNAb), virus-specific IgG or IgA, Following a second immunization 4 weeks later, a significantly increased VNAb response was noted in animals that had received vaccine doses containing 10(4) U IFN-gamma (p < 0.05). These animals also had significantly augmented serum levels of IgG (p < 0.01) and IgA (p < 0.05). Inclusion of 10(6) U IFN-gamma in the vaccine preparation did not affect the antibody response. In one experiment, the pigs were challenged oronasally with 10(5) TCID(50) of the 75V19 strain of SHV-1, 7 weeks after administration of the second vaccine dose. Those that had received 10(4) U IFN-gamma in the vaccination developed less fever during the postchallenge period (p < 0.004). In all challenged pigs, growth performance was compromised during the first week after challenge. However, the only animals retaining an average net increase in body mass were those covaccinated with 104 U IFN-gamma (p < 0.05). Nasal excretion of virus was not significantly different between groups that had been vaccinated with or without IFN-gamma. Multiple linear regression analysis of variables from individual vaccinated animals revealed the VNAb response to be correlated with serum IgG levels (p < 0.025) and with postchallenge growth performance (p < 0.0001) but not with serum IgA levels (p > 0.5), On the other hand, serum IgA appeared to be inversely correlated with early nasal virus excretion after challenge (p < 0.006), Taken together, our data suggest that addition of IFN-gamma to inactivated SHV-1 vaccine may be a useful tool for enhancement of both mucosal and systemic immune responses in pigs.
Keywords
INTERFERON-GAMMA, AUJESZKYS-DISEASE, PSEUDORABIES VIRUS, IMMUNE-RESPONSES, CYTOKINES, ENHANCEMENT, INFECTION, PROTEIN, SWINE

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Chicago
Vandenbroeck, Koen, Hans Nauwynck, Anita Vanderpooten, Kristien Van Reeth, Bruno Goddeeris, and Alfons Billiau. 1998. “Recombinant Porcine IFN-γ Potentiates the Secondary IgG and IgA Responses to an Inactivated Suid Herpesvirus-1 Vaccine and Reduces Postchallenge Weight Loss and Fever in Pigs.” Journal of Interferon and Cytokine Research 18 (9): 739–744.
APA
Vandenbroeck, K., Nauwynck, H., Vanderpooten, A., Van Reeth, K., Goddeeris, B., & Billiau, A. (1998). Recombinant porcine IFN-γ potentiates the secondary IgG and IgA responses to an inactivated suid herpesvirus-1 vaccine and reduces postchallenge weight loss and fever in pigs. JOURNAL OF INTERFERON AND CYTOKINE RESEARCH, 18(9), 739–744.
Vancouver
1.
Vandenbroeck K, Nauwynck H, Vanderpooten A, Van Reeth K, Goddeeris B, Billiau A. Recombinant porcine IFN-γ potentiates the secondary IgG and IgA responses to an inactivated suid herpesvirus-1 vaccine and reduces postchallenge weight loss and fever in pigs. JOURNAL OF INTERFERON AND CYTOKINE RESEARCH. 1998;18(9):739–44.
MLA
Vandenbroeck, Koen, Hans Nauwynck, Anita Vanderpooten, et al. “Recombinant Porcine IFN-γ Potentiates the Secondary IgG and IgA Responses to an Inactivated Suid Herpesvirus-1 Vaccine and Reduces Postchallenge Weight Loss and Fever in Pigs.” JOURNAL OF INTERFERON AND CYTOKINE RESEARCH 18.9 (1998): 739–744. Print.
@article{180139,
  abstract     = {The effect of recombinant porcine interferon-gamma (IFN-gamma) on the immunogenicity in vivo of inactivated suid herpesvirus-1 (SHV-1, Phylaxia strain) was studied applying two successive i.m, immunizations. The animals were injected with inactivated virus alone or inactivated virus supplemented with 10(4) or 10(6) U IFN-gamma. After the first immunization, none of the animals responded with measurable virus-neutralizing antibody (VNAb), virus-specific IgG or IgA, Following a second immunization 4 weeks later, a significantly increased VNAb response was noted in animals that had received vaccine doses containing 10(4) U IFN-gamma (p {\textlangle} 0.05). These animals also had significantly augmented serum levels of IgG (p {\textlangle} 0.01) and IgA (p {\textlangle} 0.05). Inclusion of 10(6) U IFN-gamma in the vaccine preparation did not affect the antibody response. In one experiment, the pigs were challenged oronasally with 10(5) TCID(50) of the 75V19 strain of SHV-1, 7 weeks after administration of the second vaccine dose. Those that had received 10(4) U IFN-gamma in the vaccination developed less fever during the postchallenge period (p {\textlangle} 0.004). In all challenged pigs, growth performance was compromised during the first week after challenge. However, the only animals retaining an average net increase in body mass were those covaccinated with 104 U IFN-gamma (p {\textlangle} 0.05). Nasal excretion of virus was not significantly different between groups that had been vaccinated with or without IFN-gamma. Multiple linear regression analysis of variables from individual vaccinated animals revealed the VNAb response to be correlated with serum IgG levels (p {\textlangle} 0.025) and with postchallenge growth performance (p {\textlangle} 0.0001) but not with serum IgA levels (p {\textrangle} 0.5), On the other hand, serum IgA appeared to be inversely correlated with early nasal virus excretion after challenge (p {\textlangle} 0.006), Taken together, our data suggest that addition of IFN-gamma to inactivated SHV-1 vaccine may be a useful tool for enhancement of both mucosal and systemic immune responses in pigs.},
  author       = {Vandenbroeck, Koen and Nauwynck, Hans and Vanderpooten, Anita and Van Reeth, Kristien and Goddeeris, Bruno and Billiau, Alfons},
  issn         = {1079-9907},
  journal      = {JOURNAL OF INTERFERON AND CYTOKINE RESEARCH},
  keyword      = {INTERFERON-GAMMA,AUJESZKYS-DISEASE,PSEUDORABIES VIRUS,IMMUNE-RESPONSES,CYTOKINES,ENHANCEMENT,INFECTION,PROTEIN,SWINE},
  language     = {eng},
  number       = {9},
  pages        = {739--744},
  title        = {Recombinant porcine IFN-\ensuremath{\gamma} potentiates the secondary IgG and IgA responses to an inactivated suid herpesvirus-1 vaccine and reduces postchallenge weight loss and fever in pigs},
  url          = {http://dx.doi.org/10.1089/jir.1998.18.739},
  volume       = {18},
  year         = {1998},
}

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