Processing of a chimeric protein in chloroplasts is different in transgenic maize and tobacco plants
- Author
- Frank Van Breusegem (UGent) , Sergei Kushnir (UGent) , Luit Slooten, Guy Bauw (UGent) , Johan Botterman (UGent) , Marc Van Montagu (UGent) and Dirk Inzé (UGent)
- Organization
- Abstract
- Transgenic maize (Zea mays L.) and tobacco (Nicotiana tabacum Petit Havana SR1) plants have been generated, which overproduce a mitochondrial Nicotiana plumbaginifolia manganese superoxide dismutase (MnSOD) in chloroplasts. For this, the mature MnSOD-coding sequence was fused to a chloroplast transit peptide from a Pisum sativum ribulose-1,5-bisphosphate carboxylase (Rubisco) gene and expression of the chimeric gene was driven by the cauliflower mosaic virus (CaMV) 35S promoter. The transgenic MnSOD gene product was correctly targeted to the chloroplasts both in maize and tobacco. However, despite the use of the CaMV 35S promoter, the MnSOD was predominantly localized in the chloroplasts of the bundle sheath cells of maize. Furthermore, the transit peptide was cleaved off at a different position in maize and tobacco.
- Keywords
- chloroplast transit peptide, CaMV 35S, manganese superoxide dismutase, post-translational modification, processing, transgenic maize, RIBULOSE-1, 5-BISPHOSPHATE CARBOXYLASE-OXYGENASE, LARGE SUBUNIT, PHOSPHOENOLPYRUVATE CARBOXYLASE, PRECURSOR PROTEINS, TRANSIT PEPTIDES, PHOSPHORYLATION, CLEAVAGE, BINDING, MOTIF, CELLS
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Citation
Please use this url to cite or link to this publication: http://hdl.handle.net/1854/LU-179830
- MLA
- Van Breusegem, Frank, et al. “Processing of a Chimeric Protein in Chloroplasts Is Different in Transgenic Maize and Tobacco Plants.” PLANT MOLECULAR BIOLOGY, vol. 38, no. 3, 1998, pp. 491–96, doi:10.1023/A:1006032110409.
- APA
- Van Breusegem, F., Kushnir, S., Slooten, L., Bauw, G., Botterman, J., Van Montagu, M., & Inzé, D. (1998). Processing of a chimeric protein in chloroplasts is different in transgenic maize and tobacco plants. PLANT MOLECULAR BIOLOGY, 38(3), 491–496. https://doi.org/10.1023/A:1006032110409
- Chicago author-date
- Van Breusegem, Frank, Sergei Kushnir, Luit Slooten, Guy Bauw, Johan Botterman, Marc Van Montagu, and Dirk Inzé. 1998. “Processing of a Chimeric Protein in Chloroplasts Is Different in Transgenic Maize and Tobacco Plants.” PLANT MOLECULAR BIOLOGY 38 (3): 491–96. https://doi.org/10.1023/A:1006032110409.
- Chicago author-date (all authors)
- Van Breusegem, Frank, Sergei Kushnir, Luit Slooten, Guy Bauw, Johan Botterman, Marc Van Montagu, and Dirk Inzé. 1998. “Processing of a Chimeric Protein in Chloroplasts Is Different in Transgenic Maize and Tobacco Plants.” PLANT MOLECULAR BIOLOGY 38 (3): 491–496. doi:10.1023/A:1006032110409.
- Vancouver
- 1.Van Breusegem F, Kushnir S, Slooten L, Bauw G, Botterman J, Van Montagu M, et al. Processing of a chimeric protein in chloroplasts is different in transgenic maize and tobacco plants. PLANT MOLECULAR BIOLOGY. 1998;38(3):491–6.
- IEEE
- [1]F. Van Breusegem et al., “Processing of a chimeric protein in chloroplasts is different in transgenic maize and tobacco plants,” PLANT MOLECULAR BIOLOGY, vol. 38, no. 3, pp. 491–496, 1998.
@article{179830,
abstract = {{Transgenic maize (Zea mays L.) and tobacco (Nicotiana tabacum Petit Havana SR1) plants have been generated, which overproduce a mitochondrial Nicotiana plumbaginifolia manganese superoxide dismutase (MnSOD) in chloroplasts. For this, the mature MnSOD-coding sequence was fused to a chloroplast transit peptide from a Pisum sativum ribulose-1,5-bisphosphate carboxylase (Rubisco) gene and expression of the chimeric gene was driven by the cauliflower mosaic virus (CaMV) 35S promoter. The transgenic MnSOD gene product was correctly targeted to the chloroplasts both in maize and tobacco. However, despite the use of the CaMV 35S promoter, the MnSOD was predominantly localized in the chloroplasts of the bundle sheath cells of maize. Furthermore, the transit peptide was cleaved off at a different position in maize and tobacco.}},
author = {{Van Breusegem, Frank and Kushnir, Sergei and Slooten, Luit and Bauw, Guy and Botterman, Johan and Van Montagu, Marc and Inzé, Dirk}},
issn = {{0167-4412}},
journal = {{PLANT MOLECULAR BIOLOGY}},
keywords = {{chloroplast transit peptide,CaMV 35S,manganese superoxide dismutase,post-translational modification,processing,transgenic maize,RIBULOSE-1,5-BISPHOSPHATE CARBOXYLASE-OXYGENASE,LARGE SUBUNIT,PHOSPHOENOLPYRUVATE CARBOXYLASE,PRECURSOR PROTEINS,TRANSIT PEPTIDES,PHOSPHORYLATION,CLEAVAGE,BINDING,MOTIF,CELLS}},
language = {{eng}},
number = {{3}},
pages = {{491--496}},
title = {{Processing of a chimeric protein in chloroplasts is different in transgenic maize and tobacco plants}},
url = {{http://doi.org/10.1023/A:1006032110409}},
volume = {{38}},
year = {{1998}},
}
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