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Murine adseverin (D5), a novel member of the gelsolin family, and murine adseverin are induced by interleukin-9 in T-helper lymphocytes

Johan Robbens UGent, Jamila Louahed, Kathleen De Pestel, Inge Van Colen UGent, Christophe Ampe UGent, Joël Vandekerckhove UGent and Jean-Christophe Renauld (1998) MOLECULAR AND CELLULAR BIOLOGY. 18(8). p.4589-4596
abstract
We identified a number of upregulated genes by differential screening of interleukin-9-stimulated T-helper lymphocytes. Interestingly, two of these messengers encode proteins that are similar to proteins of the gelsolin family. The first displays a typical structure of six homologous domains and shows a high level of identity (90%) with bovine adseverin (or scinderin) and may therefore be considered the murine adseverin homolog. The second encodes a protein with only five segments. Sequence comparison shows that most of the fifth segment and a short amino-terminal part of the sixth segment (amino acids 528 to 628 of adseverin) are missing, and thus, this form may represent an alternatively spliced product derived from the same gene. The corresponding protein is called mouse adseverin (D5). We expressed both proteins in Escherichia coli and show that mouse adseverin displays the typical characteristics of all members of the gelsolin family with respect to actin binding (capping, severing, and nucleation) and its regulation by Ca2+. In contrast, mouse adseverin (D5) fails to nucleate actin polymerization, although like mouse adseverin and gelsolin, it severs and caps actin filaments in a Ca2+-dependent manner. Adseverin is present in all of the tissues and most of the cell lines tested, although at low concentrations. Mouse adseverin (D5) was found only in blood cells and in cell lines derived from T-helper lymphocytes and mast cells, where it is weakly expressed. In a gel filtration experiment, we demonstrated that mouse adseverin forms a 1:2 complex with G actin which is stable only in the presence of Ca2+, while no stable complex was observed for mouse adseverin (D5).
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
FILAMENT-SEVERING PROTEIN, ACTIN-BINDING-SITES, CELL GROWTH-FACTOR, SEQUENCE-ANALYSIS, ESCHERICHIA-COLI, PLASMA GELSOLIN, PHYSARUM ACTIN, EXPRESSION, CALCIUM, IDENTIFICATION
journal title
MOLECULAR AND CELLULAR BIOLOGY
Mol. Cell. Biol.
volume
18
issue
8
pages
4589 - 4596
Web of Science type
Article
Web of Science id
000074950000019
ISSN
0270-7306
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
179129
handle
http://hdl.handle.net/1854/LU-179129
date created
2004-01-14 13:41:00
date last changed
2013-01-30 09:43:23
@article{179129,
  abstract     = {We identified a number of upregulated genes by differential screening of interleukin-9-stimulated T-helper lymphocytes. Interestingly, two of these messengers encode proteins that are similar to proteins of the gelsolin family. The first displays a typical structure of six homologous domains and shows a high level of identity (90\%) with bovine adseverin (or scinderin) and may therefore be considered the murine adseverin homolog. The second encodes a protein with only five segments. Sequence comparison shows that most of the fifth segment and a short amino-terminal part of the sixth segment (amino acids 528 to 628 of adseverin) are missing, and thus, this form may represent an alternatively spliced product derived from the same gene. The corresponding protein is called mouse adseverin (D5). We expressed both proteins in Escherichia coli and show that mouse adseverin displays the typical characteristics of all members of the gelsolin family with respect to actin binding (capping, severing, and nucleation) and its regulation by Ca2+. In contrast, mouse adseverin (D5) fails to nucleate actin polymerization, although like mouse adseverin and gelsolin, it severs and caps actin filaments in a Ca2+-dependent manner. Adseverin is present in all of the tissues and most of the cell lines tested, although at low concentrations. Mouse adseverin (D5) was found only in blood cells and in cell lines derived from T-helper lymphocytes and mast cells, where it is weakly expressed. In a gel filtration experiment, we demonstrated that mouse adseverin forms a 1:2 complex with G actin which is stable only in the presence of Ca2+, while no stable complex was observed for mouse adseverin (D5).},
  author       = {Robbens, Johan and Louahed, Jamila and De Pestel, Kathleen and Van Colen, Inge and Ampe, Christophe and Vandekerckhove, Jo{\"e}l and Renauld, Jean-Christophe},
  issn         = {0270-7306},
  journal      = {MOLECULAR AND CELLULAR BIOLOGY},
  keyword      = {FILAMENT-SEVERING PROTEIN,ACTIN-BINDING-SITES,CELL GROWTH-FACTOR,SEQUENCE-ANALYSIS,ESCHERICHIA-COLI,PLASMA GELSOLIN,PHYSARUM ACTIN,EXPRESSION,CALCIUM,IDENTIFICATION},
  language     = {eng},
  number       = {8},
  pages        = {4589--4596},
  title        = {Murine adseverin (D5), a novel member of the gelsolin family, and murine adseverin are induced by interleukin-9 in T-helper lymphocytes},
  volume       = {18},
  year         = {1998},
}

Chicago
Robbens, Johan, Jamila Louahed, Kathleen De Pestel, Inge Van Colen, Christophe Ampe, Joël Vandekerckhove, and Jean-Christophe Renauld. 1998. “Murine Adseverin (D5), a Novel Member of the Gelsolin Family, and Murine Adseverin Are Induced by Interleukin-9 in T-helper Lymphocytes.” Molecular and Cellular Biology 18 (8): 4589–4596.
APA
Robbens, J., Louahed, J., De Pestel, K., Van Colen, I., Ampe, C., Vandekerckhove, J., & Renauld, J.-C. (1998). Murine adseverin (D5), a novel member of the gelsolin family, and murine adseverin are induced by interleukin-9 in T-helper lymphocytes. MOLECULAR AND CELLULAR BIOLOGY, 18(8), 4589–4596.
Vancouver
1.
Robbens J, Louahed J, De Pestel K, Van Colen I, Ampe C, Vandekerckhove J, et al. Murine adseverin (D5), a novel member of the gelsolin family, and murine adseverin are induced by interleukin-9 in T-helper lymphocytes. MOLECULAR AND CELLULAR BIOLOGY. 1998;18(8):4589–96.
MLA
Robbens, Johan, Jamila Louahed, Kathleen De Pestel, et al. “Murine Adseverin (D5), a Novel Member of the Gelsolin Family, and Murine Adseverin Are Induced by Interleukin-9 in T-helper Lymphocytes.” MOLECULAR AND CELLULAR BIOLOGY 18.8 (1998): 4589–4596. Print.