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Locating the petunia Rf gene on a 650-kb DNA fragment

(1998) THEORETICAL AND APPLIED GENETICS. 96(6-7). p.980-988
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Abstract
A bulked segregant analysis was conducted in order to find RAPD and AFLP markers linked to the restorer of fertility (Rf) gene in petunia. One RAPD marker, OP704, and one AFLP marker, ECCA/ MACT, were found to be closely linked to Rf(<1 cM) in our mapping population produced from an intraspecific Petunia hybrida cross. These two single-copy markers bracketing Rf were then mapped as RFLPs on the tomato map. Despite some rearrangement: between the petunia and the tomato genomes, this synteny survey revealed two tomato markers, TG250 and CT24, closely linked to Rf: Physical mapping indicates that CT24, OP704 and ECCA/MACT lie on the same 650-kb Milli fragment. A physical to genetic distance ratio of 400 kb/cM around the Rf gene should make it feasible to identify markers physically very close to Rf.
Keywords
MARKERS, TOMATO, IDENTIFICATION, CONSTRUCTION, REGION, LOCUS, RICE, AFLP, LINKAGE MAPS, DISEASE-RESISTANCE GENES, mapping, Rf, molecular marker, petunia, synteny

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Citation

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MLA
Bentolila, S, Johannes Zethof, Tom Gerats, et al. “Locating the Petunia Rf Gene on a 650-kb DNA Fragment.” THEORETICAL AND APPLIED GENETICS 96.6-7 (1998): 980–988. Print.
APA
Bentolila, S., Zethof, J., Gerats, T., & Hanson, M. (1998). Locating the petunia Rf gene on a 650-kb DNA fragment. THEORETICAL AND APPLIED GENETICS, 96(6-7), 980–988.
Chicago author-date
Bentolila, S, Johannes Zethof, Tom Gerats, and MR Hanson. 1998. “Locating the Petunia Rf Gene on a 650-kb DNA Fragment.” Theoretical and Applied Genetics 96 (6-7): 980–988.
Chicago author-date (all authors)
Bentolila, S, Johannes Zethof, Tom Gerats, and MR Hanson. 1998. “Locating the Petunia Rf Gene on a 650-kb DNA Fragment.” Theoretical and Applied Genetics 96 (6-7): 980–988.
Vancouver
1.
Bentolila S, Zethof J, Gerats T, Hanson M. Locating the petunia Rf gene on a 650-kb DNA fragment. THEORETICAL AND APPLIED GENETICS. 1998;96(6-7):980–8.
IEEE
[1]
S. Bentolila, J. Zethof, T. Gerats, and M. Hanson, “Locating the petunia Rf gene on a 650-kb DNA fragment,” THEORETICAL AND APPLIED GENETICS, vol. 96, no. 6–7, pp. 980–988, 1998.
@article{178476,
  abstract     = {A bulked segregant analysis was conducted in order to find RAPD and AFLP markers linked to the restorer of fertility (Rf) gene in petunia. One RAPD marker, OP704, and one AFLP marker, ECCA/ MACT, were found to be closely linked to Rf(<1 cM) in our mapping population produced from an intraspecific Petunia hybrida cross. These two single-copy markers bracketing Rf were then mapped as RFLPs on the tomato map. Despite some rearrangement: between the petunia and the tomato genomes, this synteny survey revealed two tomato markers, TG250 and CT24, closely linked to Rf: Physical mapping indicates that CT24, OP704 and ECCA/MACT lie on the same 650-kb Milli fragment. A physical to genetic distance ratio of 400 kb/cM around the Rf gene should make it feasible to identify markers physically very close to Rf.},
  author       = {Bentolila, S and Zethof, Johannes and Gerats, Tom and Hanson, MR},
  issn         = {0040-5752},
  journal      = {THEORETICAL AND APPLIED GENETICS},
  keywords     = {MARKERS,TOMATO,IDENTIFICATION,CONSTRUCTION,REGION,LOCUS,RICE,AFLP,LINKAGE MAPS,DISEASE-RESISTANCE GENES,mapping,Rf,molecular marker,petunia,synteny},
  language     = {eng},
  number       = {6-7},
  pages        = {980--988},
  title        = {Locating the petunia Rf gene on a 650-kb DNA fragment},
  url          = {http://dx.doi.org/10.1007/s001220050829},
  volume       = {96},
  year         = {1998},
}

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