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5'- and 3'-sequences of satellite tobacco necrosis virus RNA promoting translation in tobacco

Frank Meulewaeter UGent, Xavier Danthinne, Marc Van Montagu UGent and Marc Cornelissen (1998) PLANT JOURNAL. 14(2). p.169-176
abstract
The RNA of satellite tobacco necrosis virus (STNV) is a monocistronic messenger that lacks both a 5' cap and a 3' poly(A) tail. The STNV trailer contains an autonomous translational enhancer domain (TED) that promotes translation in vitro by more than one order of magnitude when combined with the 5'-terminal 173 nt of STNV RNA. We now show that the responsible sequence within the 5' region maps to the first 38 nt of the STNV RNA. Mutational analysis indicated that the primary sequence of the STNV 5' 38 nt and TED is important far translation stimulation in vitro, but did not reveal a role for the complementarity between the two. Translation of chimeric STNV-cat RNAs in tobacco protoplasts showed that TED promotes translation in vivo of RNAs lacking a cap and/or a poly(A) tail. Similar to in vitro, TED-dependent translation in tobacco was stimulated further by the STNV 5' 38 nt.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
CAP, EFFICIENCY, EXPRESSION, SEQUENCE, REGION, MESSENGER-RNA, INTERNAL INITIATION, STIMULATES TRANSLATION, PICORNAVIRUS RNA, POLY(A) TAIL
journal title
PLANT JOURNAL
Plant J.
volume
14
issue
2
pages
169 - 176
Web of Science type
Article
Web of Science id
000073794300004
ISSN
0960-7412
DOI
10.1046/j.1365-313X.1998.00104.x
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
178245
handle
http://hdl.handle.net/1854/LU-178245
date created
2004-01-14 13:41:00
date last changed
2016-12-19 15:38:08
@article{178245,
  abstract     = {The RNA of satellite tobacco necrosis virus (STNV) is a monocistronic messenger that lacks both a 5' cap and a 3' poly(A) tail. The STNV trailer contains an autonomous translational enhancer domain (TED) that promotes translation in vitro by more than one order of magnitude when combined with the 5'-terminal 173 nt of STNV RNA. We now show that the responsible sequence within the 5' region maps to the first 38 nt of the STNV RNA. Mutational analysis indicated that the primary sequence of the STNV 5' 38 nt and TED is important far translation stimulation in vitro, but did not reveal a role for the complementarity between the two. Translation of chimeric STNV-cat RNAs in tobacco protoplasts showed that TED promotes translation in vivo of RNAs lacking a cap and/or a poly(A) tail. Similar to in vitro, TED-dependent translation in tobacco was stimulated further by the STNV 5' 38 nt.},
  author       = {Meulewaeter, Frank and Danthinne, Xavier and Van Montagu, Marc and Cornelissen, Marc},
  issn         = {0960-7412},
  journal      = {PLANT JOURNAL},
  keyword      = {CAP,EFFICIENCY,EXPRESSION,SEQUENCE,REGION,MESSENGER-RNA,INTERNAL INITIATION,STIMULATES TRANSLATION,PICORNAVIRUS RNA,POLY(A) TAIL},
  language     = {eng},
  number       = {2},
  pages        = {169--176},
  title        = {5'- and 3'-sequences of satellite tobacco necrosis virus RNA promoting translation in tobacco},
  url          = {http://dx.doi.org/10.1046/j.1365-313X.1998.00104.x},
  volume       = {14},
  year         = {1998},
}

Chicago
Meulewaeter, Frank, Xavier Danthinne, Marc Van Montagu, and Marc Cornelissen. 1998. “5’- and 3'-sequences of Satellite Tobacco Necrosis Virus RNA Promoting Translation in Tobacco.” Plant Journal 14 (2): 169–176.
APA
Meulewaeter, F., Danthinne, X., Van Montagu, M., & Cornelissen, M. (1998). 5’- and 3'-sequences of satellite tobacco necrosis virus RNA promoting translation in tobacco. PLANT JOURNAL, 14(2), 169–176.
Vancouver
1.
Meulewaeter F, Danthinne X, Van Montagu M, Cornelissen M. 5’- and 3'-sequences of satellite tobacco necrosis virus RNA promoting translation in tobacco. PLANT JOURNAL. 1998;14(2):169–76.
MLA
Meulewaeter, Frank, Xavier Danthinne, Marc Van Montagu, et al. “5’- and 3'-sequences of Satellite Tobacco Necrosis Virus RNA Promoting Translation in Tobacco.” PLANT JOURNAL 14.2 (1998): 169–176. Print.