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In vitro expression of adhesion receptors and diapedesis by polymorphonuclear neutrophils during experimentally induced Streptococcus uberis mastitis.

(1998) INFECTION AND IMMUNITY. 66(6). p.2529-2534
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Abstract
The expression of adhesion receptors and diapedesis by polymorphonuclear neutrophils (PMN) were studied before and during experimentally induced Streptococcus uberis mastitis. Both quarters of the left half of the udders of five midlactation cows were inoculated with a suspension containing approximately 500 CFU of S. uberis 0140J. Clinical signs of an inflammatory reaction and leukocyte influx were observed 24 h after challenge. The expression of CD11b/CD18 adhesion receptors, determined by flow cytometry, was upregulated 24 h after challenge. A confluent monolayer of bovine secretory mammary epithelial cells on collagen-coated inserts was used to study PMN diapedesis. Bovine C5a was used as the chemoattractant. An 80% decrease in PMN diapedesis was observed 24 h after challenge. The decrease in diapedesis continued for 3 weeks after challenge.
Keywords
VIRAL DIARRHEA VIRUS, TUMOR-NECROSIS-FACTOR, ESCHERICHIA-COLI MASTITIS, BOVINE NEUTROPHILS, LEUKOCYTE FUNCTION, FACTOR-ALPHA, STAPHYLOCOCCUS-AUREUS, PATHOLOGICAL FINDINGS, L-SELECTIN, MIGRATION

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Citation

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Chicago
Smits, Elke, Christian Burvenich, AJ Guidry, and Eddy Roets. 1998. “In Vitro Expression of Adhesion Receptors and Diapedesis by Polymorphonuclear Neutrophils During Experimentally Induced Streptococcus Uberis Mastitis.” Infection and Immunity 66 (6): 2529–2534.
APA
Smits, E., Burvenich, C., Guidry, A., & Roets, E. (1998). In vitro expression of adhesion receptors and diapedesis by polymorphonuclear neutrophils during experimentally induced Streptococcus uberis mastitis. INFECTION AND IMMUNITY, 66(6), 2529–2534.
Vancouver
1.
Smits E, Burvenich C, Guidry A, Roets E. In vitro expression of adhesion receptors and diapedesis by polymorphonuclear neutrophils during experimentally induced Streptococcus uberis mastitis. INFECTION AND IMMUNITY. 1998;66(6):2529–34.
MLA
Smits, Elke, Christian Burvenich, AJ Guidry, et al. “In Vitro Expression of Adhesion Receptors and Diapedesis by Polymorphonuclear Neutrophils During Experimentally Induced Streptococcus Uberis Mastitis.” INFECTION AND IMMUNITY 66.6 (1998): 2529–2534. Print.
@article{178199,
  abstract     = {The expression of adhesion receptors and diapedesis by polymorphonuclear neutrophils (PMN) were studied before and during experimentally induced Streptococcus uberis mastitis. Both quarters of the left half of the udders of five midlactation cows were inoculated with a suspension containing approximately 500 CFU of S. uberis 0140J. Clinical signs of an inflammatory reaction and leukocyte influx were observed 24 h after challenge. The expression of CD11b/CD18 adhesion receptors, determined by flow cytometry, was upregulated 24 h after challenge. A confluent monolayer of bovine secretory mammary epithelial cells on collagen-coated inserts was used to study PMN diapedesis. Bovine C5a was used as the chemoattractant. An 80\% decrease in PMN diapedesis was observed 24 h after challenge. The decrease in diapedesis continued for 3 weeks after challenge.},
  author       = {Smits, Elke and Burvenich, Christian and Guidry, AJ and Roets, Eddy},
  issn         = {0019-9567},
  journal      = {INFECTION AND IMMUNITY},
  keyword      = {VIRAL DIARRHEA VIRUS,TUMOR-NECROSIS-FACTOR,ESCHERICHIA-COLI MASTITIS,BOVINE NEUTROPHILS,LEUKOCYTE FUNCTION,FACTOR-ALPHA,STAPHYLOCOCCUS-AUREUS,PATHOLOGICAL FINDINGS,L-SELECTIN,MIGRATION},
  language     = {eng},
  number       = {6},
  pages        = {2529--2534},
  title        = {In vitro expression of adhesion receptors and diapedesis by polymorphonuclear neutrophils during experimentally induced Streptococcus uberis mastitis.},
  url          = {http://iai.asm.org/cgi/reprint/66/6/2529},
  volume       = {66},
  year         = {1998},
}

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