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Plant regeneration from embryo-derived callus in Phaseolus vulgaris L. (common bean) and P. acutifolius A Gray (tepary bean)

Mukund Zambre (UGent) , Janniek De Clercq (UGent) , Eva Vranová (UGent) , Marc Van Montagu (UGent) , Geert Angenon (UGent) and Willy Dillen (UGent)
(1998) PLANT CELL REPORTS. 17(8). p.626-630
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Abstract
Regeneration-competent callus of Phaseolus vulgaris and P. acutifolius was obtained from mature embryo explants on a medium containing thidiazuron and indole-3-acetic acid. For the P. vulgaris genotype Xan-159, regeneration was achieved from cotyledon explants, but not from embryonic axis explants. Both explants could be used for the P. acutifolius genotype NI 574 but embryonic axes gave the best results. In-vitro-rooted plantlets of P. acutifolius could readily be established in the greenhouse. For P. vulgaris hardening problems with in-vitro-rooted plantlets could be overcome by means of in vitro grafting. The potential of the described procedure for ob raining transgenic P. vulgaris plants is discussed.
Keywords
in vitro regeneration, Fabaceae, in vitro grafting, legumes, organogenesis, PISUM-SATIVUM L, SHOOT ORGANOGENESIS, EXPLANTS, TRANSFORMATION, THIDIAZURON

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Chicago
Zambre, Mukund, Janniek De Clercq, Eva Vranová, Marc Van Montagu, Geert Angenon, and Willy Dillen. 1998. “Plant Regeneration from Embryo-derived Callus in Phaseolus Vulgaris L. (common Bean) and P. Acutifolius A Gray (tepary Bean).” Plant Cell Reports 17 (8): 626–630.
APA
Zambre, M., De Clercq, J., Vranová, E., Van Montagu, M., Angenon, G., & Dillen, W. (1998). Plant regeneration from embryo-derived callus in Phaseolus vulgaris L. (common bean) and P. acutifolius A Gray (tepary bean). PLANT CELL REPORTS, 17(8), 626–630.
Vancouver
1.
Zambre M, De Clercq J, Vranová E, Van Montagu M, Angenon G, Dillen W. Plant regeneration from embryo-derived callus in Phaseolus vulgaris L. (common bean) and P. acutifolius A Gray (tepary bean). PLANT CELL REPORTS. 1998;17(8):626–30.
MLA
Zambre, Mukund, Janniek De Clercq, Eva Vranová, et al. “Plant Regeneration from Embryo-derived Callus in Phaseolus Vulgaris L. (common Bean) and P. Acutifolius A Gray (tepary Bean).” PLANT CELL REPORTS 17.8 (1998): 626–630. Print.
@article{178124,
  abstract     = {Regeneration-competent callus of Phaseolus vulgaris and P. acutifolius was obtained from mature embryo explants on a medium containing thidiazuron and indole-3-acetic acid. For the P. vulgaris genotype Xan-159, regeneration was achieved from cotyledon explants, but not from embryonic axis explants. Both explants could be used for the P. acutifolius genotype NI 574 but embryonic axes gave the best results. In-vitro-rooted plantlets of P. acutifolius could readily be established in the greenhouse. For P. vulgaris hardening problems with in-vitro-rooted plantlets could be overcome by means of in vitro grafting. The potential of the described procedure for ob raining transgenic P. vulgaris plants is discussed.},
  author       = {Zambre, Mukund and De Clercq, Janniek and Vranov{\'a}, Eva and Van Montagu, Marc and Angenon, Geert and Dillen, Willy},
  issn         = {0721-7714},
  journal      = {PLANT CELL REPORTS},
  keyword      = {in vitro regeneration,Fabaceae,in vitro grafting,legumes,organogenesis,PISUM-SATIVUM L,SHOOT ORGANOGENESIS,EXPLANTS,TRANSFORMATION,THIDIAZURON},
  language     = {eng},
  number       = {8},
  pages        = {626--630},
  title        = {Plant regeneration from embryo-derived callus in Phaseolus vulgaris L. (common bean) and P. acutifolius A Gray (tepary bean)},
  url          = {http://dx.doi.org/10.1007/s002990050455},
  volume       = {17},
  year         = {1998},
}

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