Plant regeneration from embryo-derived callus in Phaseolus vulgaris L. (common bean) and P. acutifolius A Gray (tepary bean)
- Author
- Mukund Zambre (UGent) , Janniek De Clercq (UGent) , Eva Vranová (UGent) , Marc Van Montagu (UGent) , Geert Angenon (UGent) and Willy Dillen (UGent)
- Organization
- Abstract
- Regeneration-competent callus of Phaseolus vulgaris and P. acutifolius was obtained from mature embryo explants on a medium containing thidiazuron and indole-3-acetic acid. For the P. vulgaris genotype Xan-159, regeneration was achieved from cotyledon explants, but not from embryonic axis explants. Both explants could be used for the P. acutifolius genotype NI 574 but embryonic axes gave the best results. In-vitro-rooted plantlets of P. acutifolius could readily be established in the greenhouse. For P. vulgaris hardening problems with in-vitro-rooted plantlets could be overcome by means of in vitro grafting. The potential of the described procedure for ob raining transgenic P. vulgaris plants is discussed.
- Keywords
- in vitro regeneration, Fabaceae, in vitro grafting, legumes, organogenesis, PISUM-SATIVUM L, SHOOT ORGANOGENESIS, EXPLANTS, TRANSFORMATION, THIDIAZURON
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Citation
Please use this url to cite or link to this publication: http://hdl.handle.net/1854/LU-178124
- MLA
- Zambre, Mukund, et al. “Plant Regeneration from Embryo-Derived Callus in Phaseolus Vulgaris L. (Common Bean) and P. Acutifolius A Gray (Tepary Bean).” PLANT CELL REPORTS, vol. 17, no. 8, 1998, pp. 626–30, doi:10.1007/s002990050455.
- APA
- Zambre, M., De Clercq, J., Vranová, E., Van Montagu, M., Angenon, G., & Dillen, W. (1998). Plant regeneration from embryo-derived callus in Phaseolus vulgaris L. (common bean) and P. acutifolius A Gray (tepary bean). PLANT CELL REPORTS, 17(8), 626–630. https://doi.org/10.1007/s002990050455
- Chicago author-date
- Zambre, Mukund, Janniek De Clercq, Eva Vranová, Marc Van Montagu, Geert Angenon, and Willy Dillen. 1998. “Plant Regeneration from Embryo-Derived Callus in Phaseolus Vulgaris L. (Common Bean) and P. Acutifolius A Gray (Tepary Bean).” PLANT CELL REPORTS 17 (8): 626–30. https://doi.org/10.1007/s002990050455.
- Chicago author-date (all authors)
- Zambre, Mukund, Janniek De Clercq, Eva Vranová, Marc Van Montagu, Geert Angenon, and Willy Dillen. 1998. “Plant Regeneration from Embryo-Derived Callus in Phaseolus Vulgaris L. (Common Bean) and P. Acutifolius A Gray (Tepary Bean).” PLANT CELL REPORTS 17 (8): 626–630. doi:10.1007/s002990050455.
- Vancouver
- 1.Zambre M, De Clercq J, Vranová E, Van Montagu M, Angenon G, Dillen W. Plant regeneration from embryo-derived callus in Phaseolus vulgaris L. (common bean) and P. acutifolius A Gray (tepary bean). PLANT CELL REPORTS. 1998;17(8):626–30.
- IEEE
- [1]M. Zambre, J. De Clercq, E. Vranová, M. Van Montagu, G. Angenon, and W. Dillen, “Plant regeneration from embryo-derived callus in Phaseolus vulgaris L. (common bean) and P. acutifolius A Gray (tepary bean),” PLANT CELL REPORTS, vol. 17, no. 8, pp. 626–630, 1998.
@article{178124,
abstract = {{Regeneration-competent callus of Phaseolus vulgaris and P. acutifolius was obtained from mature embryo explants on a medium containing thidiazuron and indole-3-acetic acid. For the P. vulgaris genotype Xan-159, regeneration was achieved from cotyledon explants, but not from embryonic axis explants. Both explants could be used for the P. acutifolius genotype NI 574 but embryonic axes gave the best results. In-vitro-rooted plantlets of P. acutifolius could readily be established in the greenhouse. For P. vulgaris hardening problems with in-vitro-rooted plantlets could be overcome by means of in vitro grafting. The potential of the described procedure for ob raining transgenic P. vulgaris plants is discussed.}},
author = {{Zambre, Mukund and De Clercq, Janniek and Vranová, Eva and Van Montagu, Marc and Angenon, Geert and Dillen, Willy}},
issn = {{0721-7714}},
journal = {{PLANT CELL REPORTS}},
keywords = {{in vitro regeneration,Fabaceae,in vitro grafting,legumes,organogenesis,PISUM-SATIVUM L,SHOOT ORGANOGENESIS,EXPLANTS,TRANSFORMATION,THIDIAZURON}},
language = {{eng}},
number = {{8}},
pages = {{626--630}},
title = {{Plant regeneration from embryo-derived callus in Phaseolus vulgaris L. (common bean) and P. acutifolius A Gray (tepary bean)}},
url = {{http://doi.org/10.1007/s002990050455}},
volume = {{17}},
year = {{1998}},
}
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