Advanced search
1 file | 419.01 KB

Defense activation and enhanced pathogen tolerance induced by H₂O₂ in transgenic tobacco

Author
Organization
Abstract
Transgenic tobacco deficient in the H2O2-removing enzyme catalase (Cat1AS) was used as an inducible and noninvasive system to study the role of H2O2 as an activator of pathogenesis-related (PR) proteins in plants, Excess H2O2 in Cat1AS plants was generated by simply increasing light intensities, Sustained exposure of Cat1AS plants to excess H2O2 provoked tissue damage, stimulated salicylic acid and ethylene production, and induced the expression of acidic and basic PR proteins with a timing and magnitude similar to the hypersensitive response against pathogens. Salicylic acid production was biphasic, and the first peak of salicylic acid as well as the peal of ethylene occurred within the first hours of high light, which is long before the development of tissue necrosis, Under these conditions, accumulation of acidic PR proteins was also seen in upper leaves that were not exposed to high light, indicating systemic induction of expression, Short exposure of Cat1AS plants to excess H2O2 did not cause damage, induced local expression of acidic and basic PR proteins, and enhanced pathogen tolerance. However, the timing and magnitude of PR protein induction was inn this case more similar to that in upper uninfected leaves than to that in hypersensitive-response leaves of pathogen-infected plants, Together, these data demonstrate that sublethal levels of H2O2 activate expression of acidic and basic PR proteins and lead to enhanced pathogen tolerance. However, rapid and strong activation of PR protein expression, as seen during the hypersensitive response, occurs only when excess H2O2 is accompanied by leaf necrosis.

Downloads

  • (...).pdf
    • full text
    • |
    • UGent only
    • |
    • PDF
    • |
    • 419.01 KB

Citation

Please use this url to cite or link to this publication:

Chicago
Chamnongpol, Sangpen, Hilde Willekens, Wolfgang Moeder, Christian Langebartels, Heinrich Sandermann Jr, Marc Van Montagu, Dirk Inzé, and Wim Van Camp. 1998. “Defense Activation and Enhanced Pathogen Tolerance Induced by H₂O₂ in Transgenic Tobacco.” Proceedings of the National Academy of Sciences of the United States of America 95 (10): 5818–5823.
APA
Chamnongpol, S., Willekens, H., Moeder, W., Langebartels, C., Sandermann, H., Jr, Van Montagu, M., Inzé, D., et al. (1998). Defense activation and enhanced pathogen tolerance induced by H₂O₂ in transgenic tobacco. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 95(10), 5818–5823.
Vancouver
1.
Chamnongpol S, Willekens H, Moeder W, Langebartels C, Sandermann H Jr, Van Montagu M, et al. Defense activation and enhanced pathogen tolerance induced by H₂O₂ in transgenic tobacco. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA. 1998;95(10):5818–23.
MLA
Chamnongpol, Sangpen, Hilde Willekens, Wolfgang Moeder, et al. “Defense Activation and Enhanced Pathogen Tolerance Induced by H₂O₂ in Transgenic Tobacco.” PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 95.10 (1998): 5818–5823. Print.
@article{178040,
  abstract     = {Transgenic tobacco deficient in the H2O2-removing enzyme catalase (Cat1AS) was used as an inducible and noninvasive system to study the role of H2O2 as an activator of pathogenesis-related (PR) proteins in plants, Excess H2O2 in Cat1AS plants was generated by simply increasing light intensities, Sustained exposure of Cat1AS plants to excess H2O2 provoked tissue damage, stimulated salicylic acid and ethylene production, and induced the expression of acidic and basic PR proteins with a timing and magnitude similar to the hypersensitive response against pathogens. Salicylic acid production was biphasic, and the first peak of salicylic acid as well as the peal of ethylene occurred within the first hours of high light, which is long before the development of tissue necrosis, Under these conditions, accumulation of acidic PR proteins was also seen in upper leaves that were not exposed to high light, indicating systemic induction of expression, Short exposure of Cat1AS plants to excess H2O2 did not cause damage, induced local expression of acidic and basic PR proteins, and enhanced pathogen tolerance. However, the timing and magnitude of PR protein induction was inn this case more similar to that in upper uninfected leaves than to that in hypersensitive-response leaves of pathogen-infected plants, Together, these data demonstrate that sublethal levels of H2O2 activate expression of acidic and basic PR proteins and lead to enhanced pathogen tolerance. However, rapid and strong activation of PR protein expression, as seen during the hypersensitive response, occurs only when excess H2O2 is accompanied by leaf necrosis.},
  author       = {Chamnongpol, Sangpen and Willekens, Hilde and Moeder, Wolfgang and Langebartels, Christian and Sandermann, Heinrich, Jr and Van Montagu, Marc and Inz{\'e}, Dirk and Van Camp, Wim},
  issn         = {0027-8424},
  journal      = {PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA},
  language     = {eng},
  number       = {10},
  pages        = {5818--5823},
  title        = {Defense activation and enhanced pathogen tolerance induced by H\unmatched{2082}O\unmatched{2082} in transgenic tobacco},
  url          = {http://dx.doi.org/10.1073/pnas.95.10.5818},
  volume       = {95},
  year         = {1998},
}

Altmetric
View in Altmetric
Web of Science
Times cited: