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Extracellular β-1,3-glucanases are induced during early somatic embryogenesis in Cichorium

Stéphane Helleboid, Guy Bauw UGent, Lionel Belingheri, Jacques Vasseur and Jean-Louis Hilbert (1998) PLANTA. 205(1). p.56-63
abstract
In leaf tissues of the Cichorium hybrid clone '474' (C. intybus L. var. sativum x C. endivia L. var. latifolia), the acquisition and expression of embryogenic competence was characterised by the appearance of 15 polypeptides (Boyer et al., 1993, Plant Sci 93: 41-53). The 38-kDa proteins were found to be abundantly present in conditioned embryogenic medium after the first division of the induced cells. These proteins seemed to be glycosylated as indicated by general carbohydrate detection methods. Internal amino-acid sequences obtained after microsequencing tryptic peptides appeared to be 36-57% homologous with plant beta-1,3-endoglucanases. In addition, these 38-kDa proteins were recognised by antibodies raised against the pathogenesis-related tobacco glucanase PR2a and their beta-1,3-glucanase activity was demonstrated by direct detection in polyacrylamide gels after electrophoresis. These results strongly suggested that the 38-kDa somatic-embryogenesis-related (SER) polypeptides are beta-1,3-glucanases. Moreover, the level of glucanase activity was nearly three times higher in the medium of the embryogenic '474' line than in the medium of a non-embryogenic line. The possible involvement of the extracellular 38-kDa proteins in callose degradation during somatic embryogenesis is discussed.
Please use this url to cite or link to this publication:
author
organization
alternative title
Extracellular beta-1,3-glucanases are induced during early somatic embryogenesis in Cichorium
year
type
journalArticle (original)
publication status
published
subject
keyword
ELECTROPHORESIS, ACCUMULATION, GENE, CHITINASE, PEA TISSUE, TOBACCO, PROTEIN-PATTERNS, ACQUIRED-RESISTANCE, POLYACRYLAMIDE GELS, ANTIFUNGAL HYDROLASES, Cichorium, extracellular polypeptide, beta-1, 3-glucanase, microsequencing, somatic embryogenesis
journal title
PLANTA
Planta
volume
205
issue
1
pages
56 - 63
Web of Science type
Article
Web of Science id
000073551800007
ISSN
0032-0935
DOI
10.1007/s004250050296
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
178034
handle
http://hdl.handle.net/1854/LU-178034
date created
2004-01-14 13:41:00
date last changed
2016-12-19 15:46:09
@article{178034,
  abstract     = {In leaf tissues of the Cichorium hybrid clone '474' (C. intybus L. var. sativum x C. endivia L. var. latifolia), the acquisition and expression of embryogenic competence was characterised by the appearance of 15 polypeptides (Boyer et al., 1993, Plant Sci 93: 41-53). The 38-kDa proteins were found to be abundantly present in conditioned embryogenic medium after the first division of the induced cells. These proteins seemed to be glycosylated as indicated by general carbohydrate detection methods. Internal amino-acid sequences obtained after microsequencing tryptic peptides appeared to be 36-57\% homologous with plant beta-1,3-endoglucanases. In addition, these 38-kDa proteins were recognised by antibodies raised against the pathogenesis-related tobacco glucanase PR2a and their beta-1,3-glucanase activity was demonstrated by direct detection in polyacrylamide gels after electrophoresis. These results strongly suggested that the 38-kDa somatic-embryogenesis-related (SER) polypeptides are beta-1,3-glucanases. Moreover, the level of glucanase activity was nearly three times higher in the medium of the embryogenic '474' line than in the medium of a non-embryogenic line. The possible involvement of the extracellular 38-kDa proteins in callose degradation during somatic embryogenesis is discussed.},
  author       = {Helleboid, St{\'e}phane and Bauw, Guy and Belingheri, Lionel and Vasseur, Jacques and Hilbert, Jean-Louis},
  issn         = {0032-0935},
  journal      = {PLANTA},
  keyword      = {ELECTROPHORESIS,ACCUMULATION,GENE,CHITINASE,PEA TISSUE,TOBACCO,PROTEIN-PATTERNS,ACQUIRED-RESISTANCE,POLYACRYLAMIDE GELS,ANTIFUNGAL HYDROLASES,Cichorium,extracellular polypeptide,beta-1,3-glucanase,microsequencing,somatic embryogenesis},
  language     = {eng},
  number       = {1},
  pages        = {56--63},
  title        = {Extracellular \ensuremath{\beta}-1,3-glucanases are induced during early somatic embryogenesis in Cichorium},
  url          = {http://dx.doi.org/10.1007/s004250050296},
  volume       = {205},
  year         = {1998},
}

Chicago
Helleboid, Stéphane, Guy Bauw, Lionel Belingheri, Jacques Vasseur, and Jean-Louis Hilbert. 1998. “Extracellular Β-1,3-glucanases Are Induced During Early Somatic Embryogenesis in Cichorium.” Planta 205 (1): 56–63.
APA
Helleboid, S., Bauw, G., Belingheri, L., Vasseur, J., & Hilbert, J.-L. (1998). Extracellular β-1,3-glucanases are induced during early somatic embryogenesis in Cichorium. PLANTA, 205(1), 56–63.
Vancouver
1.
Helleboid S, Bauw G, Belingheri L, Vasseur J, Hilbert J-L. Extracellular β-1,3-glucanases are induced during early somatic embryogenesis in Cichorium. PLANTA. 1998;205(1):56–63.
MLA
Helleboid, Stéphane, Guy Bauw, Lionel Belingheri, et al. “Extracellular Β-1,3-glucanases Are Induced During Early Somatic Embryogenesis in Cichorium.” PLANTA 205.1 (1998): 56–63. Print.