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Immunohistochemical detection of porcine epidemic diarrhea virus compared to other methods

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Abstract
An immunohistochemistry method using formalin-fixed tissues, a direct immunofluorescence method using cryostat sections, an enzyme-linked immunosorbent assay (ELISA), and a PCR method were compared for diagnosis in a litter of weaned pigs that had been experimentally inoculated with wild-type porcine epidemic diarrhea virus (PEDV) and killed between 6 and 60 h after onset of diarrhea. The immunohistochemistry method proved to be as reliable as direct immunofluorescence for diagnosis of PEDV in tissues collected postmortem. The good reliability of ELISA for investigating clinical samples was confirmed, whereas the PCR method used was ineffective.
Keywords
ANTIBODIES, CORONAVIRUS, INFECTION, PIGS

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Chicago
Guscetti, Franco, Curzio Bernasconi, Kurt Tobler, Kristien Van Reeth, Andreas Pospischil, and Mathias Ackermann. 1998. “Immunohistochemical Detection of Porcine Epidemic Diarrhea Virus Compared to Other Methods.” Clinical and Diagnostic Laboratory Immunology 5 (3): 412–414.
APA
Guscetti, F., Bernasconi, C., Tobler, K., Van Reeth, K., Pospischil, A., & Ackermann, M. (1998). Immunohistochemical detection of porcine epidemic diarrhea virus compared to other methods. CLINICAL AND DIAGNOSTIC LABORATORY IMMUNOLOGY, 5(3), 412–414.
Vancouver
1.
Guscetti F, Bernasconi C, Tobler K, Van Reeth K, Pospischil A, Ackermann M. Immunohistochemical detection of porcine epidemic diarrhea virus compared to other methods. CLINICAL AND DIAGNOSTIC LABORATORY IMMUNOLOGY. 1998;5(3):412–4.
MLA
Guscetti, Franco, Curzio Bernasconi, Kurt Tobler, et al. “Immunohistochemical Detection of Porcine Epidemic Diarrhea Virus Compared to Other Methods.” CLINICAL AND DIAGNOSTIC LABORATORY IMMUNOLOGY 5.3 (1998): 412–414. Print.
@article{177847,
  abstract     = {An immunohistochemistry method using formalin-fixed tissues, a direct immunofluorescence method using cryostat sections, an enzyme-linked immunosorbent assay (ELISA), and a PCR method were compared for diagnosis in a litter of weaned pigs that had been experimentally inoculated with wild-type porcine epidemic diarrhea virus (PEDV) and killed between 6 and 60 h after onset of diarrhea. The immunohistochemistry method proved to be as reliable as direct immunofluorescence for diagnosis of PEDV in tissues collected postmortem. The good reliability of ELISA for investigating clinical samples was confirmed, whereas the PCR method used was ineffective.},
  author       = {Guscetti, Franco and Bernasconi, Curzio and Tobler, Kurt and Van Reeth, Kristien and Pospischil, Andreas and Ackermann, Mathias},
  issn         = {1071-412X},
  journal      = {CLINICAL AND DIAGNOSTIC LABORATORY IMMUNOLOGY},
  keyword      = {ANTIBODIES,CORONAVIRUS,INFECTION,PIGS},
  language     = {eng},
  number       = {3},
  pages        = {412--414},
  title        = {Immunohistochemical detection of porcine epidemic diarrhea virus compared to other methods},
  url          = {http://www.ncbi.nlm.nih.gov/pmc/articles/PMC104535/pdf/cd000412.pdf},
  volume       = {5},
  year         = {1998},
}

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