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Immunohistochemical detection of porcine epidemic diarrhea virus compared to other methods

Franco Guscetti, Curzio Bernasconi, Kurt Tobler, Kristien Van Reeth UGent, Andreas Pospischil and Mathias Ackermann (1998) CLINICAL AND DIAGNOSTIC LABORATORY IMMUNOLOGY. 5(3). p.412-414
abstract
An immunohistochemistry method using formalin-fixed tissues, a direct immunofluorescence method using cryostat sections, an enzyme-linked immunosorbent assay (ELISA), and a PCR method were compared for diagnosis in a litter of weaned pigs that had been experimentally inoculated with wild-type porcine epidemic diarrhea virus (PEDV) and killed between 6 and 60 h after onset of diarrhea. The immunohistochemistry method proved to be as reliable as direct immunofluorescence for diagnosis of PEDV in tissues collected postmortem. The good reliability of ELISA for investigating clinical samples was confirmed, whereas the PCR method used was ineffective.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
ANTIBODIES, CORONAVIRUS, INFECTION, PIGS
journal title
CLINICAL AND DIAGNOSTIC LABORATORY IMMUNOLOGY
Clin. Diagn. Lab. Immunol.
volume
5
issue
3
pages
412 - 414
Web of Science type
Article
Web of Science id
000073471300028
ISSN
1071-412X
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
177847
handle
http://hdl.handle.net/1854/LU-177847
alternative location
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC104535/pdf/cd000412.pdf
date created
2004-01-14 13:41:00
date last changed
2016-12-19 15:38:59
@article{177847,
  abstract     = {An immunohistochemistry method using formalin-fixed tissues, a direct immunofluorescence method using cryostat sections, an enzyme-linked immunosorbent assay (ELISA), and a PCR method were compared for diagnosis in a litter of weaned pigs that had been experimentally inoculated with wild-type porcine epidemic diarrhea virus (PEDV) and killed between 6 and 60 h after onset of diarrhea. The immunohistochemistry method proved to be as reliable as direct immunofluorescence for diagnosis of PEDV in tissues collected postmortem. The good reliability of ELISA for investigating clinical samples was confirmed, whereas the PCR method used was ineffective.},
  author       = {Guscetti, Franco and Bernasconi, Curzio and Tobler, Kurt and Van Reeth, Kristien and Pospischil, Andreas and Ackermann, Mathias},
  issn         = {1071-412X},
  journal      = {CLINICAL AND DIAGNOSTIC LABORATORY IMMUNOLOGY},
  keyword      = {ANTIBODIES,CORONAVIRUS,INFECTION,PIGS},
  language     = {eng},
  number       = {3},
  pages        = {412--414},
  title        = {Immunohistochemical detection of porcine epidemic diarrhea virus compared to other methods},
  url          = {http://www.ncbi.nlm.nih.gov/pmc/articles/PMC104535/pdf/cd000412.pdf},
  volume       = {5},
  year         = {1998},
}

Chicago
Guscetti, Franco, Curzio Bernasconi, Kurt Tobler, Kristien Van Reeth, Andreas Pospischil, and Mathias Ackermann. 1998. “Immunohistochemical Detection of Porcine Epidemic Diarrhea Virus Compared to Other Methods.” Clinical and Diagnostic Laboratory Immunology 5 (3): 412–414.
APA
Guscetti, F., Bernasconi, C., Tobler, K., Van Reeth, K., Pospischil, A., & Ackermann, M. (1998). Immunohistochemical detection of porcine epidemic diarrhea virus compared to other methods. CLINICAL AND DIAGNOSTIC LABORATORY IMMUNOLOGY, 5(3), 412–414.
Vancouver
1.
Guscetti F, Bernasconi C, Tobler K, Van Reeth K, Pospischil A, Ackermann M. Immunohistochemical detection of porcine epidemic diarrhea virus compared to other methods. CLINICAL AND DIAGNOSTIC LABORATORY IMMUNOLOGY. 1998;5(3):412–4.
MLA
Guscetti, Franco, Curzio Bernasconi, Kurt Tobler, et al. “Immunohistochemical Detection of Porcine Epidemic Diarrhea Virus Compared to Other Methods.” CLINICAL AND DIAGNOSTIC LABORATORY IMMUNOLOGY 5.3 (1998): 412–414. Print.