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Identification of cat sequences required for intron-dependent gene expression in maize cells

Nikki Rethmeier, Evelien Kramer, Marc Van Montagu UGent and Marc Cornelissen (1998) PLANT JOURNAL. 13(6). p.831-835
abstract
Transgene expression in maize cells changed from intron-independent to intron-dependent by an exact exchange of the bar coding region for that of cat. By deletion mapping an approximately 100 nucleotide sequence element at the 5' end of the cat coding region was identified that, when inserted at the translation start site of the bar gene, impaired expression. Successive inclusion of the salT intron in the 5' untranslated region (UTR) restored expression near to wild-type bar expression levels. A chimeric gfp gene, but not nptII gene, behaved similarly. These observations are in agreement with the view that intron-mediated enhancement of transgene expression does not enhance, but rather restores expression of an impaired gene.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
RNA, EFFICIENCY
journal title
PLANT JOURNAL
Plant J.
volume
13
issue
6
pages
831 - 835
Web of Science type
Article
Web of Science id
000073121500011
ISSN
0960-7412
DOI
10.1046/j.1365-313X.1998.00096.x
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
177557
handle
http://hdl.handle.net/1854/LU-177557
date created
2004-01-14 13:41:00
date last changed
2016-12-19 15:38:07
@article{177557,
  abstract     = {Transgene expression in maize cells changed from intron-independent to intron-dependent by an exact exchange of the bar coding region for that of cat. By deletion mapping an approximately 100 nucleotide sequence element at the 5' end of the cat coding region was identified that, when inserted at the translation start site of the bar gene, impaired expression. Successive inclusion of the salT intron in the 5' untranslated region (UTR) restored expression near to wild-type bar expression levels. A chimeric gfp gene, but not nptII gene, behaved similarly. These observations are in agreement with the view that intron-mediated enhancement of transgene expression does not enhance, but rather restores expression of an impaired gene.},
  author       = {Rethmeier, Nikki and Kramer, Evelien and Van Montagu, Marc and Cornelissen, Marc},
  issn         = {0960-7412},
  journal      = {PLANT JOURNAL},
  keyword      = {RNA,EFFICIENCY},
  language     = {eng},
  number       = {6},
  pages        = {831--835},
  title        = {Identification of cat sequences required for intron-dependent gene expression in maize cells},
  url          = {http://dx.doi.org/10.1046/j.1365-313X.1998.00096.x},
  volume       = {13},
  year         = {1998},
}

Chicago
Rethmeier, Nikki, Evelien Kramer, Marc Van Montagu, and Marc Cornelissen. 1998. “Identification of Cat Sequences Required for Intron-dependent Gene Expression in Maize Cells.” Plant Journal 13 (6): 831–835.
APA
Rethmeier, N., Kramer, E., Van Montagu, M., & Cornelissen, M. (1998). Identification of cat sequences required for intron-dependent gene expression in maize cells. PLANT JOURNAL, 13(6), 831–835.
Vancouver
1.
Rethmeier N, Kramer E, Van Montagu M, Cornelissen M. Identification of cat sequences required for intron-dependent gene expression in maize cells. PLANT JOURNAL. 1998;13(6):831–5.
MLA
Rethmeier, Nikki, Evelien Kramer, Marc Van Montagu, et al. “Identification of Cat Sequences Required for Intron-dependent Gene Expression in Maize Cells.” PLANT JOURNAL 13.6 (1998): 831–835. Print.