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Identification of cat sequences required for intron-dependent gene expression in maize cells

(1998) PLANT JOURNAL. 13(6). p.831-835
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Abstract
Transgene expression in maize cells changed from intron-independent to intron-dependent by an exact exchange of the bar coding region for that of cat. By deletion mapping an approximately 100 nucleotide sequence element at the 5' end of the cat coding region was identified that, when inserted at the translation start site of the bar gene, impaired expression. Successive inclusion of the salT intron in the 5' untranslated region (UTR) restored expression near to wild-type bar expression levels. A chimeric gfp gene, but not nptII gene, behaved similarly. These observations are in agreement with the view that intron-mediated enhancement of transgene expression does not enhance, but rather restores expression of an impaired gene.
Keywords
RNA, EFFICIENCY

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Citation

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Chicago
Rethmeier, Nikki, Evelien Kramer, Marc Van Montagu, and Marc Cornelissen. 1998. “Identification of Cat Sequences Required for Intron-dependent Gene Expression in Maize Cells.” Plant Journal 13 (6): 831–835.
APA
Rethmeier, N., Kramer, E., Van Montagu, M., & Cornelissen, M. (1998). Identification of cat sequences required for intron-dependent gene expression in maize cells. PLANT JOURNAL, 13(6), 831–835.
Vancouver
1.
Rethmeier N, Kramer E, Van Montagu M, Cornelissen M. Identification of cat sequences required for intron-dependent gene expression in maize cells. PLANT JOURNAL. 1998;13(6):831–5.
MLA
Rethmeier, Nikki, Evelien Kramer, Marc Van Montagu, et al. “Identification of Cat Sequences Required for Intron-dependent Gene Expression in Maize Cells.” PLANT JOURNAL 13.6 (1998): 831–835. Print.
@article{177557,
  abstract     = {Transgene expression in maize cells changed from intron-independent to intron-dependent by an exact exchange of the bar coding region for that of cat. By deletion mapping an approximately 100 nucleotide sequence element at the 5' end of the cat coding region was identified that, when inserted at the translation start site of the bar gene, impaired expression. Successive inclusion of the salT intron in the 5' untranslated region (UTR) restored expression near to wild-type bar expression levels. A chimeric gfp gene, but not nptII gene, behaved similarly. These observations are in agreement with the view that intron-mediated enhancement of transgene expression does not enhance, but rather restores expression of an impaired gene.},
  author       = {Rethmeier, Nikki and Kramer, Evelien and Van Montagu, Marc and Cornelissen, Marc},
  issn         = {0960-7412},
  journal      = {PLANT JOURNAL},
  keyword      = {RNA,EFFICIENCY},
  language     = {eng},
  number       = {6},
  pages        = {831--835},
  title        = {Identification of cat sequences required for intron-dependent gene expression in maize cells},
  url          = {http://dx.doi.org/10.1046/j.1365-313X.1998.00096.x},
  volume       = {13},
  year         = {1998},
}

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