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Reduction of acyloxyacyl hydrolase activity in circulating neutrophils from cows after parturition

(1998) JOURNAL OF DAIRY SCIENCE. 81(3). p.672-677
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Abstract
Bovine neutrophils contain the enzyme acyloxyacyl hydrolase, which hydrolyzes the acyloxyacyl linkage of the two nonhydroxylated fatty acyl chains to two 3-hydroxy fatty acids in the highly conserved lipid A part of endotoxins with high specificity. This hydrolysis decreases the toxicity of lipid A, but the immunostimulatory capacity of endotoxins is largely maintained. In two trials, we studied the activity of acyloxyacyl hydrolase in neutrophils that had been isolated from the blood of 18 dairy cows around parturition. Between 10 and 26 d after parturition, the activity of acyloxyacyl hydrolase in neutrophils decreased approximately 20% below prepartum activity. At about 2 mo after parturition, acyloxyacyl hydrolase activity returned to prepartum values. Changes in acyloxyacyl hydrolase activity could not be attributed to changes in binding of lipopolysaccharides by the CD14 molecules on neutrophils or monocytes. We hypothesize that decreased acyloxyacyl hydrolase activity in neutrophils shortly after parturition is a factor that increases the susceptibility of dairy cows to coliform mastitis during early lactation.
Keywords
CD14, BINDING, neutrophils, mastitis, endotoxin, BACTERIAL LIPOPOLYSACCHARIDES, ESCHERICHIA-COLI MASTITIS, POLYMORPHONUCLEAR LEUKOCYTES, ENZYMATIC DEACYLATION, ENDOTOXIN, BOVINE MASTITIS, PROTEIN, INFLAMMATION

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Citation

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Chicago
Dosogne, Hilde, Anthony Capuco, Max Paape, Eddy Roets, Christian Burvenich, and B Fenwick. 1998. “Reduction of Acyloxyacyl Hydrolase Activity in Circulating Neutrophils from Cows After Parturition.” Journal of Dairy Science 81 (3): 672–677.
APA
Dosogne, Hilde, Capuco, A., Paape, M., Roets, E., Burvenich, C., & Fenwick, B. (1998). Reduction of acyloxyacyl hydrolase activity in circulating neutrophils from cows after parturition. JOURNAL OF DAIRY SCIENCE, 81(3), 672–677.
Vancouver
1.
Dosogne H, Capuco A, Paape M, Roets E, Burvenich C, Fenwick B. Reduction of acyloxyacyl hydrolase activity in circulating neutrophils from cows after parturition. JOURNAL OF DAIRY SCIENCE. 1998;81(3):672–7.
MLA
Dosogne, Hilde, Anthony Capuco, Max Paape, et al. “Reduction of Acyloxyacyl Hydrolase Activity in Circulating Neutrophils from Cows After Parturition.” JOURNAL OF DAIRY SCIENCE 81.3 (1998): 672–677. Print.
@article{177526,
  abstract     = {Bovine neutrophils contain the enzyme acyloxyacyl hydrolase, which hydrolyzes the acyloxyacyl linkage of the two nonhydroxylated fatty acyl chains to two 3-hydroxy fatty acids in the highly conserved lipid A part of endotoxins with high specificity. This hydrolysis decreases the toxicity of lipid A, but the immunostimulatory capacity of endotoxins is largely maintained. In two trials, we studied the activity of acyloxyacyl hydrolase in neutrophils that had been isolated from the blood of 18 dairy cows around parturition. Between 10 and 26 d after parturition, the activity of acyloxyacyl hydrolase in neutrophils decreased approximately 20\% below prepartum activity. At about 2 mo after parturition, acyloxyacyl hydrolase activity returned to prepartum values. Changes in acyloxyacyl hydrolase activity could not be attributed to changes in binding of lipopolysaccharides by the CD14 molecules on neutrophils or monocytes. We hypothesize that decreased acyloxyacyl hydrolase activity in neutrophils shortly after parturition is a factor that increases the susceptibility of dairy cows to coliform mastitis during early lactation.},
  author       = {Dosogne, Hilde and Capuco, Anthony and Paape, Max and Roets, Eddy and Burvenich, Christian and Fenwick, B},
  issn         = {0022-0302},
  journal      = {JOURNAL OF DAIRY SCIENCE},
  keyword      = {CD14,BINDING,neutrophils,mastitis,endotoxin,BACTERIAL LIPOPOLYSACCHARIDES,ESCHERICHIA-COLI MASTITIS,POLYMORPHONUCLEAR LEUKOCYTES,ENZYMATIC DEACYLATION,ENDOTOXIN,BOVINE MASTITIS,PROTEIN,INFLAMMATION},
  language     = {eng},
  number       = {3},
  pages        = {672--677},
  title        = {Reduction of acyloxyacyl hydrolase activity in circulating neutrophils from cows after parturition},
  url          = {http://jds.fass.org/cgi/content/abstract/81/3/672},
  volume       = {81},
  year         = {1998},
}

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