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Fluorescent in-situ hybridization on human embryos showing cleavage arrest after freezing and thawing

H Laverge, Josiane Van der Elst UGent, Petra De Sutter UGent, MR Verschraegen-Spae, Anne De Paepe UGent and Marc Dhont (1998) HUMAN REPRODUCTION. 13(2). p.425-429
abstract
Our current freezing-thawing policy is to transfer only embryos that cleave further in the 24 h following thawing, The purpose of our study was to investigate the incidence of numerical abnormalities for chromosomes X, Y and 1 in blastomeres of human preimplantation embryos that survived cryopreservation but did not cleave further after thawing. A total of 63 embryos surviving a freezing-thawing cycle but not cleaving further within 24 h after thawing were screened, Of the 63 screened embryos that showed cleavage arrest 24 h after thawing, 56 embryos (88.9%) remained arrested 48 h after thawing and slightly more than half of these (29/56; 51.8%) showed further deterioration in morphological quality, Seven embryos (11.1%) showed signs of further cleavage; five embryos showed additional cleavage of one blastomere and two developed a blastocoelic cavity, Fluorescent in-situ hybridization (FISH) with three specific probes for simultaneous detection of chromosomes X, Y and 1 was performed and was successful in 60 out of 63 embryos, Of these successfully labelled embryos, 26 (43.3%) were in the diploid range: 12 (20%) were uniformly diploid for the chromosomes X, Y and 1; three embryos showed aneuploidy in all their blastomeres (two were XXY-karyotype and one was monosomy 1) and in 11 embryos nondisjunction was detected, Thirteen embryos were categorized as being either haploid, triploid, tetraploid or hexaploid, Nine embryos were classified as mosaic and 12 as being highly abnormal or chaotic, These preliminary results suggest that a large proportion of embryos that do not cleave further after freezing and thawing carry chromosomal aberrations, This finding supports our policy of not transferring cryopreserved embryos which do not cleave further 24 h following thawing.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
fluorescent in-situ hybridization, cleavage, freezing, human embryos, thawing, PREIMPLANTATION HUMAN EMBRYOS, Y-CHROMOSOME, CRYOPRESERVATION, DIMETHYLSULFOXIDE, ABNORMALITIES, MOSAICISM
journal title
HUMAN REPRODUCTION
Hum. Reprod.
volume
13
issue
2
pages
425 - 429
Web of Science type
Article
Web of Science id
000072925500040
ISSN
0268-1161
DOI
10.1093/humrep/13.2.425
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
177374
handle
http://hdl.handle.net/1854/LU-177374
date created
2004-01-14 13:41:00
date last changed
2016-12-19 15:38:06
@article{177374,
  abstract     = {Our current freezing-thawing policy is to transfer only embryos that cleave further in the 24 h following thawing, The purpose of our study was to investigate the incidence of numerical abnormalities for chromosomes X, Y and 1 in blastomeres of human preimplantation embryos that survived cryopreservation but did not cleave further after thawing. A total of 63 embryos surviving a freezing-thawing cycle but not cleaving further within 24 h after thawing were screened, Of the 63 screened embryos that showed cleavage arrest 24 h after thawing, 56 embryos (88.9\%) remained arrested 48 h after thawing and slightly more than half of these (29/56; 51.8\%) showed further deterioration in morphological quality, Seven embryos (11.1\%) showed signs of further cleavage; five embryos showed additional cleavage of one blastomere and two developed a blastocoelic cavity, Fluorescent in-situ hybridization (FISH) with three specific probes for simultaneous detection of chromosomes X, Y and 1 was performed and was successful in 60 out of 63 embryos, Of these successfully labelled embryos, 26 (43.3\%) were in the diploid range: 12 (20\%) were uniformly diploid for the chromosomes X, Y and 1; three embryos showed aneuploidy in all their blastomeres (two were XXY-karyotype and one was monosomy 1) and in 11 embryos nondisjunction was detected, Thirteen embryos were categorized as being either haploid, triploid, tetraploid or hexaploid, Nine embryos were classified as mosaic and 12 as being highly abnormal or chaotic, These preliminary results suggest that a large proportion of embryos that do not cleave further after freezing and thawing carry chromosomal aberrations, This finding supports our policy of not transferring cryopreserved embryos which do not cleave further 24 h following thawing.},
  author       = {Laverge, H and Van der Elst, Josiane and De Sutter, Petra and Verschraegen-Spae, MR and De Paepe, Anne and Dhont, Marc},
  issn         = {0268-1161},
  journal      = {HUMAN REPRODUCTION},
  keyword      = {fluorescent in-situ hybridization,cleavage,freezing,human embryos,thawing,PREIMPLANTATION HUMAN EMBRYOS,Y-CHROMOSOME,CRYOPRESERVATION,DIMETHYLSULFOXIDE,ABNORMALITIES,MOSAICISM},
  language     = {eng},
  number       = {2},
  pages        = {425--429},
  title        = {Fluorescent in-situ hybridization on human embryos showing cleavage arrest after freezing and thawing},
  url          = {http://dx.doi.org/10.1093/humrep/13.2.425},
  volume       = {13},
  year         = {1998},
}

Chicago
Laverge, H, Josiane Van der Elst, Petra De Sutter, MR Verschraegen-Spae, Anne De Paepe, and MARC DHONT. 1998. “Fluorescent In-situ Hybridization on Human Embryos Showing Cleavage Arrest After Freezing and Thawing.” Human Reproduction 13 (2): 425–429.
APA
Laverge, H., Van der Elst, J., De Sutter, P., Verschraegen-Spae, M., De Paepe, A., & DHONT, M. (1998). Fluorescent in-situ hybridization on human embryos showing cleavage arrest after freezing and thawing. HUMAN REPRODUCTION, 13(2), 425–429.
Vancouver
1.
Laverge H, Van der Elst J, De Sutter P, Verschraegen-Spae M, De Paepe A, DHONT M. Fluorescent in-situ hybridization on human embryos showing cleavage arrest after freezing and thawing. HUMAN REPRODUCTION. 1998;13(2):425–9.
MLA
Laverge, H, Josiane Van der Elst, Petra De Sutter, et al. “Fluorescent In-situ Hybridization on Human Embryos Showing Cleavage Arrest After Freezing and Thawing.” HUMAN REPRODUCTION 13.2 (1998): 425–429. Print.