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Purification of the alkaloid lycorine and simultaneous analysis of ascorbic acid and lycorine by micellar electrokinetic capillary chromatography

(1998) ANALYTICAL BIOCHEMISTRY. 257(1). p.80-88
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Abstract
The pyrrolophenanthridine alkaloid lycorine has frequently been used as a specific inhibitor to help elucidate the function of ascorbic acid (vitamin C) in a wide range of biological processes. It was recently reported that this function can be exercised by inhibiting the activity of L-galactono-1,4-lactone dehydrogenase, the terminal enzyme of ascorbic acid biosynthesis, although working with the purified enzyme, we have been unable to repeat this result. Here, we present a procedure for the purification and analysis of lycorine by high-performance liquid chromatography from two Crinum species and describe for the first time a method that allows the simultaneous analysis of ascorbic acid and lycorine in tissue extracts of Crinum asiaticum by micellar electrokinetic chromatography.
Keywords
SACCHAROMYCES-CEREVISIAE, CHEMICAL-CONSTITUENTS, DEHYDROASCORBIC ACID, OXIDATIVE STRESS, AMARYLLIDACEAE, PLANTS, RESISTANCE, METABOLISM, MITOCHONDRIAL, BIOSYNTHESIS

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Citation

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Chicago
Davey, Mark W, Geert Persiau, André De Bruyn, José Van Damme, Guy Bauw, and Marc Van Montagu. 1998. “Purification of the Alkaloid Lycorine and Simultaneous Analysis of Ascorbic Acid and Lycorine by Micellar Electrokinetic Capillary Chromatography.” Analytical Biochemistry 257 (1): 80–88.
APA
Davey, M. W., Persiau, G., De Bruyn, A., Van Damme, J., Bauw, G., & Van Montagu, M. (1998). Purification of the alkaloid lycorine and simultaneous analysis of ascorbic acid and lycorine by micellar electrokinetic capillary chromatography. ANALYTICAL BIOCHEMISTRY, 257(1), 80–88.
Vancouver
1.
Davey MW, Persiau G, De Bruyn A, Van Damme J, Bauw G, Van Montagu M. Purification of the alkaloid lycorine and simultaneous analysis of ascorbic acid and lycorine by micellar electrokinetic capillary chromatography. ANALYTICAL BIOCHEMISTRY. 1998;257(1):80–8.
MLA
Davey, Mark W, Geert Persiau, André De Bruyn, et al. “Purification of the Alkaloid Lycorine and Simultaneous Analysis of Ascorbic Acid and Lycorine by Micellar Electrokinetic Capillary Chromatography.” ANALYTICAL BIOCHEMISTRY 257.1 (1998): 80–88. Print.
@article{176831,
  abstract     = {The pyrrolophenanthridine alkaloid lycorine has frequently been used as a specific inhibitor to help elucidate the function of ascorbic acid (vitamin C) in a wide range of biological processes. It was recently reported that this function can be exercised by inhibiting the activity of L-galactono-1,4-lactone dehydrogenase, the terminal enzyme of ascorbic acid biosynthesis, although working with the purified enzyme, we have been unable to repeat this result. Here, we present a procedure for the purification and analysis of lycorine by high-performance liquid chromatography from two Crinum species and describe for the first time a method that allows the simultaneous analysis of ascorbic acid and lycorine in tissue extracts of Crinum asiaticum by micellar electrokinetic chromatography.},
  author       = {Davey, Mark W and Persiau, Geert and De Bruyn, Andr{\'e} and Van Damme, Jos{\'e} and Bauw, Guy and Van Montagu, Marc},
  issn         = {0003-2697},
  journal      = {ANALYTICAL BIOCHEMISTRY},
  keyword      = {SACCHAROMYCES-CEREVISIAE,CHEMICAL-CONSTITUENTS,DEHYDROASCORBIC ACID,OXIDATIVE STRESS,AMARYLLIDACEAE,PLANTS,RESISTANCE,METABOLISM,MITOCHONDRIAL,BIOSYNTHESIS},
  language     = {eng},
  number       = {1},
  pages        = {80--88},
  title        = {Purification of the alkaloid lycorine and simultaneous analysis of ascorbic acid and lycorine by micellar electrokinetic capillary chromatography},
  url          = {http://dx.doi.org/10.1006/abio.1997.2544},
  volume       = {257},
  year         = {1998},
}

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