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Transposon Display identifies individual transposable elements in high copy number lines

Dirk Van Den Broeck UGent, Tamara Maes UGent, Matt Sauer, Johannes Zethof UGent, Peter De Keukeleire UGent, Mariëlla D'Hauw, Marc Van Montagu UGent and Tom Gerats UGent (1998) PLANT JOURNAL. 13(1). p.121-129
abstract
The dTph1 transposable element family of Petunia hybrida line W138 consists of between 100 and 200 members. A strategy that allows simultaneous detection of individual elements is described. Sequences flanking dTph1 elements are amplified by means of a ligation-mediated PCR. The resulting fragments are locus-specific and can be analysed by polyacrylamide gel electrophoresis. One of the applications of Transposon Display is the isolation of dTph1-tagged genes. Fragments that co-segregate with a mutant phenotype can be extracted from the gel and reamplified, providing access to tagged genes, as demonstrated in a reconstruction experiment. Data on the molecular identification of a phenotypic mutant, isolated in a random tagging experiment is also presented. Upon sequencing, the obtained candidate fragment was found to be identical to part of the previously identified Fbp1 gene.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
GENETIC-CHARACTERIZATION, PETUNIA-HYBRIDA, NUCLEAR GENOME, PATTERN, LOCUS
journal title
PLANT JOURNAL
Plant J.
volume
13
issue
1
pages
121 - 129
Web of Science type
Article
Web of Science id
000071696900012
ISSN
0960-7412
DOI
10.1046/j.1365-313X.1998.00004.x
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
176446
handle
http://hdl.handle.net/1854/LU-176446
date created
2004-01-14 13:40:00
date last changed
2016-12-19 15:38:08
@article{176446,
  abstract     = {The dTph1 transposable element family of Petunia hybrida line W138 consists of between 100 and 200 members. A strategy that allows simultaneous detection of individual elements is described. Sequences flanking dTph1 elements are amplified by means of a ligation-mediated PCR. The resulting fragments are locus-specific and can be analysed by polyacrylamide gel electrophoresis. One of the applications of Transposon Display is the isolation of dTph1-tagged genes. Fragments that co-segregate with a mutant phenotype can be extracted from the gel and reamplified, providing access to tagged genes, as demonstrated in a reconstruction experiment. Data on the molecular identification of a phenotypic mutant, isolated in a random tagging experiment is also presented. Upon sequencing, the obtained candidate fragment was found to be identical to part of the previously identified Fbp1 gene.},
  author       = {Van Den Broeck, Dirk and Maes, Tamara and Sauer, Matt and Zethof, Johannes and De Keukeleire, Peter and D'Hauw, Mari{\"e}lla and Van Montagu, Marc and Gerats, Tom},
  issn         = {0960-7412},
  journal      = {PLANT JOURNAL},
  keyword      = {GENETIC-CHARACTERIZATION,PETUNIA-HYBRIDA,NUCLEAR GENOME,PATTERN,LOCUS},
  language     = {eng},
  number       = {1},
  pages        = {121--129},
  title        = {Transposon Display identifies individual transposable elements in high copy number lines},
  url          = {http://dx.doi.org/10.1046/j.1365-313X.1998.00004.x},
  volume       = {13},
  year         = {1998},
}

Chicago
Van Den Broeck, Dirk, Tamara Maes, Matt Sauer, Johannes Zethof, Peter De Keukeleire, Mariëlla D’Hauw, Marc Van Montagu, and Tom Gerats. 1998. “Transposon Display Identifies Individual Transposable Elements in High Copy Number Lines.” Plant Journal 13 (1): 121–129.
APA
Van Den Broeck, D., Maes, T., Sauer, M., Zethof, J., De Keukeleire, P., D’Hauw, M., Van Montagu, M., et al. (1998). Transposon Display identifies individual transposable elements in high copy number lines. PLANT JOURNAL, 13(1), 121–129.
Vancouver
1.
Van Den Broeck D, Maes T, Sauer M, Zethof J, De Keukeleire P, D’Hauw M, et al. Transposon Display identifies individual transposable elements in high copy number lines. PLANT JOURNAL. 1998;13(1):121–9.
MLA
Van Den Broeck, Dirk, Tamara Maes, Matt Sauer, et al. “Transposon Display Identifies Individual Transposable Elements in High Copy Number Lines.” PLANT JOURNAL 13.1 (1998): 121–129. Print.