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Transposon Display identifies individual transposable elements in high copy number lines

(1998) PLANT JOURNAL. 13(1). p.121-129
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Abstract
The dTph1 transposable element family of Petunia hybrida line W138 consists of between 100 and 200 members. A strategy that allows simultaneous detection of individual elements is described. Sequences flanking dTph1 elements are amplified by means of a ligation-mediated PCR. The resulting fragments are locus-specific and can be analysed by polyacrylamide gel electrophoresis. One of the applications of Transposon Display is the isolation of dTph1-tagged genes. Fragments that co-segregate with a mutant phenotype can be extracted from the gel and reamplified, providing access to tagged genes, as demonstrated in a reconstruction experiment. Data on the molecular identification of a phenotypic mutant, isolated in a random tagging experiment is also presented. Upon sequencing, the obtained candidate fragment was found to be identical to part of the previously identified Fbp1 gene.
Keywords
GENETIC-CHARACTERIZATION, PETUNIA-HYBRIDA, NUCLEAR GENOME, PATTERN, LOCUS

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Chicago
Van Den Broeck, Dirk, Tamara Maes, Matt Sauer, Johannes Zethof, Peter De Keukeleire, Mariëlla D’Hauw, Marc Van Montagu, and Tom Gerats. 1998. “Transposon Display Identifies Individual Transposable Elements in High Copy Number Lines.” Plant Journal 13 (1): 121–129.
APA
Van Den Broeck, D., Maes, T., Sauer, M., Zethof, J., De Keukeleire, P., D’Hauw, M., Van Montagu, M., et al. (1998). Transposon Display identifies individual transposable elements in high copy number lines. PLANT JOURNAL, 13(1), 121–129.
Vancouver
1.
Van Den Broeck D, Maes T, Sauer M, Zethof J, De Keukeleire P, D’Hauw M, et al. Transposon Display identifies individual transposable elements in high copy number lines. PLANT JOURNAL. 1998;13(1):121–9.
MLA
Van Den Broeck, Dirk, Tamara Maes, Matt Sauer, et al. “Transposon Display Identifies Individual Transposable Elements in High Copy Number Lines.” PLANT JOURNAL 13.1 (1998): 121–129. Print.
@article{176446,
  abstract     = {The dTph1 transposable element family of Petunia hybrida line W138 consists of between 100 and 200 members. A strategy that allows simultaneous detection of individual elements is described. Sequences flanking dTph1 elements are amplified by means of a ligation-mediated PCR. The resulting fragments are locus-specific and can be analysed by polyacrylamide gel electrophoresis. One of the applications of Transposon Display is the isolation of dTph1-tagged genes. Fragments that co-segregate with a mutant phenotype can be extracted from the gel and reamplified, providing access to tagged genes, as demonstrated in a reconstruction experiment. Data on the molecular identification of a phenotypic mutant, isolated in a random tagging experiment is also presented. Upon sequencing, the obtained candidate fragment was found to be identical to part of the previously identified Fbp1 gene.},
  author       = {Van Den Broeck, Dirk and Maes, Tamara and Sauer, Matt and Zethof, Johannes and De Keukeleire, Peter and D'Hauw, Mari{\"e}lla and Van Montagu, Marc and Gerats, Tom},
  issn         = {0960-7412},
  journal      = {PLANT JOURNAL},
  keyword      = {GENETIC-CHARACTERIZATION,PETUNIA-HYBRIDA,NUCLEAR GENOME,PATTERN,LOCUS},
  language     = {eng},
  number       = {1},
  pages        = {121--129},
  title        = {Transposon Display identifies individual transposable elements in high copy number lines},
  url          = {http://dx.doi.org/10.1046/j.1365-313X.1998.00004.x},
  volume       = {13},
  year         = {1998},
}

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