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Regulatory sequences of Arabidopsis drive reporter gene expression in nematode feeding structures

(1997) PLANT CELL. 9(12). p.2119-2134
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Abstract
In the quest for plant regulatory sequences capable of driving nematode-triggered effector gene expression in feeding structures, we show that promoter tagging is a valuable tool, A large collection of transgenic Arabidopsis plants was generated, They were transformed with a beta-glucuronidase gene functioning as a promoter tag, Three T-DNA constructs, pGV1047, p Delta gusBin19, and pMOG553, were used. Early responses to nematode invasion were of primary interest. Six lines exhibiting beta-glucuronidase activity in syncytia induced by the beet cyst nematode were studied. Reporter gene activation was also identified in galls induced by root knot and ectoparasitic nematodes. Time-course studies revealed that all six tags were differentially activated during the development of the feeding structure. T-DNA-flanking regions responsible for the observed responses after nematode infection were isolated and characterized for promoter activity.
Keywords
ROOT-KNOT, PLANT-PARASITIC NEMATODES, TRANSGENIC PLANTS, HETERODERA-SCHACHTII, MODEL HOST, MELOIDOGYNE-INCOGNITA, SECRETORY GRANULES, CYST NEMATODES, THALIANA, PROMOTER

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Citation

Please use this url to cite or link to this publication:

Chicago
Barthels, Nathalie, Frederique M van der Lee, Joke Klap, Oscar JM Goddijn, Mansour Karimi, Piotr Puzio, Florian MW Grundler, et al. 1997. “Regulatory Sequences of Arabidopsis Drive Reporter Gene Expression in Nematode Feeding Structures.” Plant Cell 9 (12): 2119–2134.
APA
Barthels, N., van der Lee, F. M., Klap, J., Goddijn, O. J., Karimi, M., Puzio, P., Grundler, F. M., et al. (1997). Regulatory sequences of Arabidopsis drive reporter gene expression in nematode feeding structures. PLANT CELL, 9(12), 2119–2134.
Vancouver
1.
Barthels N, van der Lee FM, Klap J, Goddijn OJ, Karimi M, Puzio P, et al. Regulatory sequences of Arabidopsis drive reporter gene expression in nematode feeding structures. PLANT CELL. 1997;9(12):2119–34.
MLA
Barthels, Nathalie, Frederique M van der Lee, Joke Klap, et al. “Regulatory Sequences of Arabidopsis Drive Reporter Gene Expression in Nematode Feeding Structures.” PLANT CELL 9.12 (1997): 2119–2134. Print.
@article{176089,
  abstract     = {In the quest for plant regulatory sequences capable of driving nematode-triggered effector gene expression in feeding structures, we show that promoter tagging is a valuable tool, A large collection of transgenic Arabidopsis plants was generated, They were transformed with a beta-glucuronidase gene functioning as a promoter tag, Three T-DNA constructs, pGV1047, p Delta gusBin19, and pMOG553, were used. Early responses to nematode invasion were of primary interest. Six lines exhibiting beta-glucuronidase activity in syncytia induced by the beet cyst nematode were studied. Reporter gene activation was also identified in galls induced by root knot and ectoparasitic nematodes. Time-course studies revealed that all six tags were differentially activated during the development of the feeding structure. T-DNA-flanking regions responsible for the observed responses after nematode infection were isolated and characterized for promoter activity.},
  author       = {Barthels, Nathalie and van der Lee, Frederique M and Klap, Joke and Goddijn, Oscar JM and Karimi, Mansour and Puzio, Piotr and Grundler, Florian MW and Ohl, Stephan A and Lindsey, Keith and Robertson, Lee and Robertson, Walter M and Van Montagu, Marc and Gheysen, Godelieve and Sijmons, Peter C},
  issn         = {1040-4651},
  journal      = {PLANT CELL},
  keyword      = {ROOT-KNOT,PLANT-PARASITIC NEMATODES,TRANSGENIC PLANTS,HETERODERA-SCHACHTII,MODEL HOST,MELOIDOGYNE-INCOGNITA,SECRETORY GRANULES,CYST NEMATODES,THALIANA,PROMOTER},
  language     = {eng},
  number       = {12},
  pages        = {2119--2134},
  title        = {Regulatory sequences of Arabidopsis drive reporter gene expression in nematode feeding structures},
  url          = {http://dx.doi.org/10.1105/tpc.9.12.2119},
  volume       = {9},
  year         = {1997},
}

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