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Fluorescence recovery after photobleaching: a versatile tool for mobility and interaction measurements in pharmaceutical research

Tom KL Meyvis, Stefaan De Smedt UGent, Patric Van Oostveldt UGent and Jo Demeester UGent (1999) PHARMACEUTICAL RESEARCH. 16(8). p.1153-1162
abstract
This review introduces the basics of fluorescence recovery after photobleaching (FRAP) from a theoretical and an instrumentational approach. The most interesting and innovative applications with a pharmaceutical point of view are briefly discussed and possible future applications are suggested. These future applications include research on the mobility of macromolecular drugs in macro- or microscopic pharmaceutical dosage forms, mobility, and binding of antitumor drugs in tumor tissue, intracellular trafficking of gene complexes and mobility of drugs in membranes prior to transmembrane penetration. The paper is also intended to be an introductory guideline to those who would like to get involved in FRAP related experimental techniques. Therefore, comprehensive details on different setups and data analysis are given, as well as a brief outline of the problems that may be encountered when performing FRAP. Overall, this review shows the great potential of FRAP in pharmaceutical research. This is complemented by our own results illustrating the possibility of performing FRAP in microscopic dosage forms (microspheres) using a high resolution Variant of FRAP.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (review)
publication status
published
subject
keyword
MICROSCOPE, TRANSPORT, GELS, PLASMA-MEMBRANE, LATERAL DIFFUSION, ROTATIONAL DIFFUSION, CYTOPLASMIC VISCOSITY, TRANSLATIONAL DIFFUSION, SCANNING MICROPHOTOLYSIS, SPATIAL FOURIER-ANALYSIS, drug delivery, diffusion, interactions, FRAP, mobility
journal title
PHARMACEUTICAL RESEARCH
Pharm. Res.
volume
16
issue
8
pages
1153 - 1162
Web of Science type
Review
Web of Science id
000082047800003
ISSN
0724-8741
DOI
10.1023/A:1011924909138
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
175573
handle
http://hdl.handle.net/1854/LU-175573
date created
2004-01-14 13:40:00
date last changed
2010-07-14 12:11:59
@article{175573,
  abstract     = {This review introduces the basics of fluorescence recovery after photobleaching (FRAP) from a theoretical and an instrumentational approach. The most interesting and innovative applications with a pharmaceutical point of view are briefly discussed and possible future applications are suggested. These future applications include research on the mobility of macromolecular drugs in macro- or microscopic pharmaceutical dosage forms, mobility, and binding of antitumor drugs in tumor tissue, intracellular trafficking of gene complexes and mobility of drugs in membranes prior to transmembrane penetration. The paper is also intended to be an introductory guideline to those who would like to get involved in FRAP related experimental techniques. Therefore, comprehensive details on different setups and data analysis are given, as well as a brief outline of the problems that may be encountered when performing FRAP. Overall, this review shows the great potential of FRAP in pharmaceutical research. This is complemented by our own results illustrating the possibility of performing FRAP in microscopic dosage forms (microspheres) using a high resolution Variant of FRAP.},
  author       = {Meyvis, Tom KL and De Smedt, Stefaan and Van Oostveldt, Patric and Demeester, Jo},
  issn         = {0724-8741},
  journal      = {PHARMACEUTICAL RESEARCH},
  keyword      = {MICROSCOPE,TRANSPORT,GELS,PLASMA-MEMBRANE,LATERAL DIFFUSION,ROTATIONAL DIFFUSION,CYTOPLASMIC VISCOSITY,TRANSLATIONAL DIFFUSION,SCANNING MICROPHOTOLYSIS,SPATIAL FOURIER-ANALYSIS,drug delivery,diffusion,interactions,FRAP,mobility},
  language     = {eng},
  number       = {8},
  pages        = {1153--1162},
  title        = {Fluorescence recovery after photobleaching: a versatile tool for mobility and interaction measurements in pharmaceutical research},
  url          = {http://dx.doi.org/10.1023/A:1011924909138},
  volume       = {16},
  year         = {1999},
}

Chicago
Meyvis, Tom KL, Stefaan De Smedt, Patric Van Oostveldt, and Jo Demeester. 1999. “Fluorescence Recovery After Photobleaching: a Versatile Tool for Mobility and Interaction Measurements in Pharmaceutical Research.” Pharmaceutical Research 16 (8): 1153–1162.
APA
Meyvis, T. K., De Smedt, S., Van Oostveldt, P., & Demeester, J. (1999). Fluorescence recovery after photobleaching: a versatile tool for mobility and interaction measurements in pharmaceutical research. PHARMACEUTICAL RESEARCH, 16(8), 1153–1162.
Vancouver
1.
Meyvis TK, De Smedt S, Van Oostveldt P, Demeester J. Fluorescence recovery after photobleaching: a versatile tool for mobility and interaction measurements in pharmaceutical research. PHARMACEUTICAL RESEARCH. 1999;16(8):1153–62.
MLA
Meyvis, Tom KL, Stefaan De Smedt, Patric Van Oostveldt, et al. “Fluorescence Recovery After Photobleaching: a Versatile Tool for Mobility and Interaction Measurements in Pharmaceutical Research.” PHARMACEUTICAL RESEARCH 16.8 (1999): 1153–1162. Print.