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A novel class of ectomycorrhiza-regulated cell wall polypeptides in Pisolithus tinctorius

Pascal Laurent, Catherine Voiblet, Denis Tagu, Dulcinéia de Carvalho, Uwe Nehls, Roberta De Bellis, Raffaella Balestrini, Guy Bauw UGent, Paola Bonfante and Francis Martin (1999) MOLECULAR PLANT-MICROBE INTERACTIONS. 12(10). p.862-871
abstract
Development of the ectomycorrhizal symbiosis leads to the aggregation of fungal hyphae to form the mantle, To identify cell surface proteins involved in this developmental step, changes in the biosynthesis of fungal cell wall proteins were examined in Eucalyptus globulus-Pisolithus tinctorius ectomycorrhizas by two-dimensional polyacrylamide gel electrophoresis. Enhanced synthesis of several immunologically related fungal 31- and 32-kDa polypeptides, so-called symbiosis-regulated acidic polypeptides (SRAPs), was observed. Peptide sequences of SRAP(32d) were obtained after trypsin digestion. These peptides were found in the predicted sequence of six closely related fungal cDNAs coding for ectomycorrhiza up-regulated transcripts. The PtSRAP32 cDNAs represented about 10% of the differentially expressed cDNAs in ectomycorrhiza and are predicted to encode alanine-rich proteins of 28.2 kDa, There are no sequence homologies between SRAPs and previously identified proteins, but they contain the Arg-Gly-Asp (RGD) motif found in cell-adhesion proteins. SRAPs were observed on the hyphal surface by immunoelectron microscopy, They were also found in the host cell wall when P. tinctorius attached to the root surface. RNA blot analysis showed that the steady-state level of PtSRAP32 transcripts exhibited a drastic up-regulation when fungal hyphae form the mantle. These results suggest that SRAPs may form part of a cell-cell adhesion system needed for aggregation of hyphae in ectomycorrhizas.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
GLY-ASP MOTIF, fungus, MUSHROOM LENTINUS-EDODES, ADHESION PROTEIN MFBA, CONTAINING FRAGMENT, CANDIDA-ALBICANS, MORPHOGENESIS, HYDROPHOBINS, RGD, ELECTROPHORESIS, MYCORRHIZAS, differentiation
journal title
MOLECULAR PLANT-MICROBE INTERACTIONS
Mol. Plant-Microbe Interact.
volume
12
issue
10
pages
862 - 871
Web of Science type
Article
Web of Science id
000082827600004
ISSN
0894-0282
DOI
10.1094/MPMI.1999.12.10.862
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
174716
handle
http://hdl.handle.net/1854/LU-174716
date created
2004-01-14 13:40:00
date last changed
2016-12-19 15:38:07
@article{174716,
  abstract     = {Development of the ectomycorrhizal symbiosis leads to the aggregation of fungal hyphae to form the mantle, To identify cell surface proteins involved in this developmental step, changes in the biosynthesis of fungal cell wall proteins were examined in Eucalyptus globulus-Pisolithus tinctorius ectomycorrhizas by two-dimensional polyacrylamide gel electrophoresis. Enhanced synthesis of several immunologically related fungal 31- and 32-kDa polypeptides, so-called symbiosis-regulated acidic polypeptides (SRAPs), was observed. Peptide sequences of SRAP(32d) were obtained after trypsin digestion. These peptides were found in the predicted sequence of six closely related fungal cDNAs coding for ectomycorrhiza up-regulated transcripts. The PtSRAP32 cDNAs represented about 10\% of the differentially expressed cDNAs in ectomycorrhiza and are predicted to encode alanine-rich proteins of 28.2 kDa, There are no sequence homologies between SRAPs and previously identified proteins, but they contain the Arg-Gly-Asp (RGD) motif found in cell-adhesion proteins. SRAPs were observed on the hyphal surface by immunoelectron microscopy, They were also found in the host cell wall when P. tinctorius attached to the root surface. RNA blot analysis showed that the steady-state level of PtSRAP32 transcripts exhibited a drastic up-regulation when fungal hyphae form the mantle. These results suggest that SRAPs may form part of a cell-cell adhesion system needed for aggregation of hyphae in ectomycorrhizas.},
  author       = {Laurent, Pascal and Voiblet, Catherine and Tagu, Denis and de Carvalho, Dulcin{\'e}ia and Nehls, Uwe and De Bellis, Roberta and Balestrini, Raffaella and Bauw, Guy and Bonfante, Paola and Martin, Francis},
  issn         = {0894-0282},
  journal      = {MOLECULAR PLANT-MICROBE INTERACTIONS},
  keyword      = {GLY-ASP MOTIF,fungus,MUSHROOM LENTINUS-EDODES,ADHESION PROTEIN MFBA,CONTAINING FRAGMENT,CANDIDA-ALBICANS,MORPHOGENESIS,HYDROPHOBINS,RGD,ELECTROPHORESIS,MYCORRHIZAS,differentiation},
  language     = {eng},
  number       = {10},
  pages        = {862--871},
  title        = {A novel class of ectomycorrhiza-regulated cell wall polypeptides in Pisolithus tinctorius},
  url          = {http://dx.doi.org/10.1094/MPMI.1999.12.10.862},
  volume       = {12},
  year         = {1999},
}

Chicago
Laurent, Pascal, Catherine Voiblet, Denis Tagu, Dulcinéia de Carvalho, Uwe Nehls, Roberta De Bellis, Raffaella Balestrini, Guy Bauw, Paola Bonfante, and Francis Martin. 1999. “A Novel Class of Ectomycorrhiza-regulated Cell Wall Polypeptides in Pisolithus Tinctorius.” Molecular Plant-microbe Interactions 12 (10): 862–871.
APA
Laurent, P., Voiblet, C., Tagu, D., de Carvalho, D., Nehls, U., De Bellis, R., Balestrini, R., et al. (1999). A novel class of ectomycorrhiza-regulated cell wall polypeptides in Pisolithus tinctorius. MOLECULAR PLANT-MICROBE INTERACTIONS, 12(10), 862–871.
Vancouver
1.
Laurent P, Voiblet C, Tagu D, de Carvalho D, Nehls U, De Bellis R, et al. A novel class of ectomycorrhiza-regulated cell wall polypeptides in Pisolithus tinctorius. MOLECULAR PLANT-MICROBE INTERACTIONS. 1999;12(10):862–71.
MLA
Laurent, Pascal, Catherine Voiblet, Denis Tagu, et al. “A Novel Class of Ectomycorrhiza-regulated Cell Wall Polypeptides in Pisolithus Tinctorius.” MOLECULAR PLANT-MICROBE INTERACTIONS 12.10 (1999): 862–871. Print.