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Ultrastructure of surface components of Streptococcus gallolyticus (S. bovis) strains of differing virulence isolated from pigeons

(1999) MICROBIOLOGY-UK. 145(2). p.335-342
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Abstract
Virulence of Streptococcus gallolyticus (S. bovis) strains isolated from pigeons is associated with the presence of the extracellular proteins A, T1, T2 and T3. Based on the presence or absence of these proteins, six supernatant-phenotypes are distinguished. Experimental infection studies have indicated that strains belonging to the A(-)T1, A(+)T1, A(+)T2 and A(+)T3 groups are highly virulent for pigeons, strains belonging to the A(-)T3 groups are moderately virulent and A(-)T2 strains are of low virulence. In this study the surface structure of 15 pigeon 5. gallolyticus strains representing high, moderate and low virulence supernatant-phenotypes was examined by electron microscopy. The presence of capsular material was determined by transmission electron microscopy after polycationic ferritin labelling and immunostabilization. Capsules from cells labelled with polycationic ferritin were usually thicker than those from cells exposed to antiserum, The capsule of the virulent strains had a regular, continuous appearance whilst irregularity of the capsule was a characteristic of the low virulence A(-)T2 strains. Negative staining revealed the presence of fimbriae in all strains belonging to the high virulence A(-)T1, A(+)T1, A(+)T2 and A(+)T3 supernatant groups and in one strain of the moderately virulent A(-)T3 group. The fimbriae were thin, flexible structures with a diameter of approximately 3-4 nm and a length of up to 700 nm. Fimbriae as described above were absent in two other A(-)T3 strains examined and in the low virulence A(-)T2 strains. Results from this study indicate that morphological differences in surface structure exist among virulent and low virulence pigeon 5. gallolyticus strains, and that the capsule and/or fimbriae are possibly involved in virulence.
Keywords
Streptococcus gallolyticus (S. bovis), capsule, fimbriae, virulence, pigeon, ADHESION-DEFICIENT MUTANTS, GROUP-A STREPTOCOCCI, GROUP-B STREPTOCOCCI, RACING PIGEONS, CELL-SURFACE, M-PROTEIN, HYALURONATE CAPSULE, INFECTIONS, SALIVARIUS, FIMBRIAE

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Chicago
Vanrobaeys, Mia, Peter De Herdt, G Charlier, Richard Ducatelle, and Freddy Haesebrouck. 1999. “Ultrastructure of Surface Components of Streptococcus Gallolyticus (S. Bovis) Strains of Differing Virulence Isolated from Pigeons.” Microbiology-uk 145 (2): 335–342.
APA
Vanrobaeys, M., De Herdt, P., Charlier, G., Ducatelle, R., & Haesebrouck, F. (1999). Ultrastructure of surface components of Streptococcus gallolyticus (S. bovis) strains of differing virulence isolated from pigeons. MICROBIOLOGY-UK, 145(2), 335–342.
Vancouver
1.
Vanrobaeys M, De Herdt P, Charlier G, Ducatelle R, Haesebrouck F. Ultrastructure of surface components of Streptococcus gallolyticus (S. bovis) strains of differing virulence isolated from pigeons. MICROBIOLOGY-UK. 1999;145(2):335–42.
MLA
Vanrobaeys, Mia, Peter De Herdt, G Charlier, et al. “Ultrastructure of Surface Components of Streptococcus Gallolyticus (S. Bovis) Strains of Differing Virulence Isolated from Pigeons.” MICROBIOLOGY-UK 145.2 (1999): 335–342. Print.
@article{174132,
  abstract     = {Virulence of Streptococcus gallolyticus (S. bovis) strains isolated from pigeons is associated with the presence of the extracellular proteins A, T1, T2 and T3. Based on the presence or absence of these proteins, six supernatant-phenotypes are distinguished. Experimental infection studies have indicated that strains belonging to the A(-)T1, A(+)T1, A(+)T2 and A(+)T3 groups are highly virulent for pigeons, strains belonging to the A(-)T3 groups are moderately virulent and A(-)T2 strains are of low virulence. In this study the surface structure of 15 pigeon 5. gallolyticus strains representing high, moderate and low virulence supernatant-phenotypes was examined by electron microscopy. The presence of capsular material was determined by transmission electron microscopy after polycationic ferritin labelling and immunostabilization. Capsules from cells labelled with polycationic ferritin were usually thicker than those from cells exposed to antiserum, The capsule of the virulent strains had a regular, continuous appearance whilst irregularity of the capsule was a characteristic of the low virulence A(-)T2 strains. Negative staining revealed the presence of fimbriae in all strains belonging to the high virulence A(-)T1, A(+)T1, A(+)T2 and A(+)T3 supernatant groups and in one strain of the moderately virulent A(-)T3 group. The fimbriae were thin, flexible structures with a diameter of approximately 3-4 nm and a length of up to 700 nm. Fimbriae as described above were absent in two other A(-)T3 strains examined and in the low virulence A(-)T2 strains. Results from this study indicate that morphological differences in surface structure exist among virulent and low virulence pigeon 5. gallolyticus strains, and that the capsule and/or fimbriae are possibly involved in virulence.},
  author       = {Vanrobaeys, Mia and De Herdt, Peter and Charlier, G and Ducatelle, Richard and Haesebrouck, Freddy},
  issn         = {1350-0872},
  journal      = {MICROBIOLOGY-UK},
  keywords     = {Streptococcus gallolyticus (S. bovis),capsule,fimbriae,virulence,pigeon,ADHESION-DEFICIENT MUTANTS,GROUP-A STREPTOCOCCI,GROUP-B STREPTOCOCCI,RACING PIGEONS,CELL-SURFACE,M-PROTEIN,HYALURONATE CAPSULE,INFECTIONS,SALIVARIUS,FIMBRIAE},
  language     = {eng},
  number       = {2},
  pages        = {335--342},
  title        = {Ultrastructure of surface components of Streptococcus gallolyticus (S. bovis) strains of differing virulence isolated from pigeons},
  url          = {http://dx.doi.org/10.1099/13500872-145-2-335},
  volume       = {145},
  year         = {1999},
}

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