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Isolation of the LEMMI9 gene and promoter analysis during a compatible plant-nematode interaction

Carolina Escobar, Jan De Meutter UGent, Fabio A Aristizábal, Soledad Sanz-Alférez, Francisca F del Campo, Nathalie Barthels UGent, Walter Van der Eycken, Jef Seurinck, Marc Van Montagu UGent, Godelieve Gheysen UGent, et al. (1999) MOLECULAR PLANT-MICROBE INTERACTIONS. 12(5). p.440-449
abstract
Plant-endoparasitic root-knot nematodes feed on specialized giant cells that they induce in the vascular cylinder of susceptible plants. Although it has been established that a number of plant genes change their expression pattern during giant cell differentiation, virtually no data are available about the mechanisms involved in that change. One possibility is differential promoter recognition by the transcription factor(s) responsible for the expression of specific genes. We have isolated and characterized a genomic clone from tomato containing the promoter region of LEMMI9, one of the few plant genes that have been reported to be highly expressed in galls (predominantly in giant cells). The analysis of transgenic potato plants carrying a LEMMI9 promoter-a glucuronidase (GUS) fusion has demonstrated that the tomato promoter was activated in Meloidogyne incognita-induced galls in a heterologous system. We have located putative regulatory sequences in the promoter and have found that nuclear proteins from the galls formed specific DNA-protein complexes with the proximal region of the LEMMI9 promoter, The nuclear protein-binding sequence mapped to a region of 111 bp immediately upstream from the TATA box, This region contains a 12-bp repeat possibly involved in the formation of DNA-protein complexes, which might be related to the LEMMI9 transcriptional activation in the giant cells.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
ABSCISIC-ACID, HOST-CELL RESPONSES, RIGHTWARD TRANSCRIPTION, FEEDING STRUCTURES, INTERGENIC REGION, OSMOTIC-STRESS, PROTEINS BIND, EXPRESSION, EMBRYOGENESIS, STREAK VIRUS CONTAINS, promoter elements, DNA-binding protein(s)
journal title
MOLECULAR PLANT-MICROBE INTERACTIONS
Mol. Plant-Microbe Interact.
volume
12
issue
5
pages
440 - 449
Web of Science type
Article
Web of Science id
000079821200009
ISSN
0894-0282
DOI
10.1094/MPMI.1999.12.5.440
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
173927
handle
http://hdl.handle.net/1854/LU-173927
date created
2004-01-14 13:40:00
date last changed
2016-12-19 15:38:07
@article{173927,
  abstract     = {Plant-endoparasitic root-knot nematodes feed on specialized giant cells that they induce in the vascular cylinder of susceptible plants. Although it has been established that a number of plant genes change their expression pattern during giant cell differentiation, virtually no data are available about the mechanisms involved in that change. One possibility is differential promoter recognition by the transcription factor(s) responsible for the expression of specific genes. We have isolated and characterized a genomic clone from tomato containing the promoter region of LEMMI9, one of the few plant genes that have been reported to be highly expressed in galls (predominantly in giant cells). The analysis of transgenic potato plants carrying a LEMMI9 promoter-a glucuronidase (GUS) fusion has demonstrated that the tomato promoter was activated in Meloidogyne incognita-induced galls in a heterologous system. We have located putative regulatory sequences in the promoter and have found that nuclear proteins from the galls formed specific DNA-protein complexes with the proximal region of the LEMMI9 promoter, The nuclear protein-binding sequence mapped to a region of 111 bp immediately upstream from the TATA box, This region contains a 12-bp repeat possibly involved in the formation of DNA-protein complexes, which might be related to the LEMMI9 transcriptional activation in the giant cells.},
  author       = {Escobar, Carolina and De Meutter, Jan and Aristiz{\'a}bal, Fabio A and Sanz-Alf{\'e}rez, Soledad and del Campo, Francisca F and Barthels, Nathalie and Van der Eycken, Walter and Seurinck, Jef and Van Montagu, Marc and Gheysen, Godelieve and Fenoll, Carmen},
  issn         = {0894-0282},
  journal      = {MOLECULAR PLANT-MICROBE INTERACTIONS},
  keyword      = {ABSCISIC-ACID,HOST-CELL RESPONSES,RIGHTWARD TRANSCRIPTION,FEEDING STRUCTURES,INTERGENIC REGION,OSMOTIC-STRESS,PROTEINS BIND,EXPRESSION,EMBRYOGENESIS,STREAK VIRUS CONTAINS,promoter elements,DNA-binding protein(s)},
  language     = {eng},
  number       = {5},
  pages        = {440--449},
  title        = {Isolation of the LEMMI9 gene and promoter analysis during a compatible plant-nematode interaction},
  url          = {http://dx.doi.org/10.1094/MPMI.1999.12.5.440},
  volume       = {12},
  year         = {1999},
}

Chicago
Escobar, Carolina, Jan De Meutter, Fabio A Aristizábal, Soledad Sanz-Alférez, Francisca F del Campo, Nathalie Barthels, Walter Van der Eycken, et al. 1999. “Isolation of the LEMMI9 Gene and Promoter Analysis During a Compatible Plant-nematode Interaction.” Molecular Plant-microbe Interactions 12 (5): 440–449.
APA
Escobar, C., De Meutter, J., Aristizábal, F. A., Sanz-Alférez, S., del Campo, F. F., Barthels, N., Van der Eycken, W., et al. (1999). Isolation of the LEMMI9 gene and promoter analysis during a compatible plant-nematode interaction. MOLECULAR PLANT-MICROBE INTERACTIONS, 12(5), 440–449.
Vancouver
1.
Escobar C, De Meutter J, Aristizábal FA, Sanz-Alférez S, del Campo FF, Barthels N, et al. Isolation of the LEMMI9 gene and promoter analysis during a compatible plant-nematode interaction. MOLECULAR PLANT-MICROBE INTERACTIONS. 1999;12(5):440–9.
MLA
Escobar, Carolina, Jan De Meutter, Fabio A Aristizábal, et al. “Isolation of the LEMMI9 Gene and Promoter Analysis During a Compatible Plant-nematode Interaction.” MOLECULAR PLANT-MICROBE INTERACTIONS 12.5 (1999): 440–449. Print.