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Effect of cephapirin and mecillinam on the phagocytic and respiratory burst activity of neutrophil leukocytes isolated from bovine blood.

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Abstract
Antimicrobial therapy is the most commonly used treatment of bacterial infections in dairy cows, Polymorphonuclear neutrophil leukocytes (PMN) play an important role in the first line defence against invading bacteria and it is important that the function of PMN is not compromised by antibiotics. We investigated the in vitro effect of cephapirin, a first generation cephalosporin, and mecillinam, an amidinopenicillin with activity against mainly Gramnegative bacteria, on phagocytosis and respiratory burst activity of PMN isolated from bovine blood, After in vitro incubation of PMN with different concentrations of the antibiotics, phagocytosis was evaluated by flow cytometry and respiratory burst activity was evaluated by registration of chemiluminescence (CL) with a luminometer, None of the investigated concentrations of cephapirin and mecillinam had an effect in vitro on phagocytosis of Escherichia coli by PMN, At high concentrations (100 and 1000 mu g/mL), cephapirin and mecillinam reduced the respiratory burst activity of PMN. Part of these suppressive effects could be ascribed to oxidant scavenging, Inhibitory effects of cephapirin were stronger than mecillinam. In conclusion, cephapirin and mecillinam did not seem to affect antibacterial activity of PMN isolated from bovine blood in vitro at therapeutic concentrations.
Keywords
ANTIBIOTICS, ESCHERICHIA-COLI MASTITIS, COWS, MYELOPEROXIDASE, CHEMOTAXIS, INFECTIONS, THERAPY, HEIFERS, DEFENSE

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Citation

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Chicago
Dosogne, H, D Hoeben, Christian Burvenich, and JACM Lohuis. 1998. “Effect of Cephapirin and Mecillinam on the Phagocytic and Respiratory Burst Activity of Neutrophil Leukocytes Isolated from Bovine Blood.” Journal of Veterinary Pharmacology and Therapeutics 21 (6): 421–427.
APA
Dosogne, H, Hoeben, D., Burvenich, C., & Lohuis, J. (1998). Effect of cephapirin and mecillinam on the phagocytic and respiratory burst activity of neutrophil leukocytes isolated from bovine blood. JOURNAL OF VETERINARY PHARMACOLOGY AND THERAPEUTICS, 21(6), 421–427.
Vancouver
1.
Dosogne H, Hoeben D, Burvenich C, Lohuis J. Effect of cephapirin and mecillinam on the phagocytic and respiratory burst activity of neutrophil leukocytes isolated from bovine blood. JOURNAL OF VETERINARY PHARMACOLOGY AND THERAPEUTICS. 1998;21(6):421–7.
MLA
Dosogne, H, D Hoeben, Christian Burvenich, et al. “Effect of Cephapirin and Mecillinam on the Phagocytic and Respiratory Burst Activity of Neutrophil Leukocytes Isolated from Bovine Blood.” JOURNAL OF VETERINARY PHARMACOLOGY AND THERAPEUTICS 21.6 (1998): 421–427. Print.
@article{173747,
  abstract     = {Antimicrobial therapy is the most commonly used treatment of bacterial infections in dairy cows, Polymorphonuclear neutrophil leukocytes (PMN) play an important role in the first line defence against invading bacteria and it is important that the function of PMN is not compromised by antibiotics. We investigated the in vitro effect of cephapirin, a first generation cephalosporin, and mecillinam, an amidinopenicillin with activity against mainly Gramnegative bacteria, on phagocytosis and respiratory burst activity of PMN isolated from bovine blood, After in vitro incubation of PMN with different concentrations of the antibiotics, phagocytosis was evaluated by flow cytometry and respiratory burst activity was evaluated by registration of chemiluminescence (CL) with a luminometer, None of the investigated concentrations of cephapirin and mecillinam had an effect in vitro on phagocytosis of Escherichia coli by PMN, At high concentrations (100 and 1000 mu g/mL), cephapirin and mecillinam reduced the respiratory burst activity of PMN. Part of these suppressive effects could be ascribed to oxidant scavenging, Inhibitory effects of cephapirin were stronger than mecillinam. In conclusion, cephapirin and mecillinam did not seem to affect antibacterial activity of PMN isolated from bovine blood in vitro at therapeutic concentrations.},
  author       = {Dosogne, H and Hoeben, D and Burvenich, Christian and Lohuis, JACM},
  issn         = {0140-7783},
  journal      = {JOURNAL OF VETERINARY PHARMACOLOGY AND THERAPEUTICS},
  keyword      = {ANTIBIOTICS,ESCHERICHIA-COLI MASTITIS,COWS,MYELOPEROXIDASE,CHEMOTAXIS,INFECTIONS,THERAPY,HEIFERS,DEFENSE},
  language     = {eng},
  number       = {6},
  pages        = {421--427},
  title        = {Effect of cephapirin and mecillinam on the phagocytic and respiratory burst activity of neutrophil leukocytes isolated from bovine blood.},
  volume       = {21},
  year         = {1998},
}

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