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Oxidative stress in plants: EPR monitoring in DMPO-DMSO based extracts

Sabine Van Doorslaer UGent, Andrée Dedonder UGent, Marc De Block and Freddy Callens UGent (1999) JOURNAL OF PLANT PHYSIOLOGY. 154(1). p.132-136
abstract
Oxygen derived radicals were measured in Brassica napus hypocotyl segments by means of EPR (electron paramagnetic resonance) after spin-trapping with 5,5-dimethyl-1-pyrroline-N-oxide (DMPO) in a dimethylsulfoxide(DMSO)-based medium. Next to the ascorbyl radical signal, an EPR signal due to DMPO-CH3, generated by reaction of free OH radicals with DMPO, was detected. The DMPO-CH3 signals were quantified in Brassica napus hypocotyl segments at different times after wounding and in vitro culture. A maximum was detected as a function of time after wounding. When hypocotyl segments were kept in the dark, a reduction of about 70% of both the DMPO-CH3 and ascorbyl line intensity in comparison to the light control was observed. This suggests that the production of ascorbyl. and hydroxyl radicals is associated with photooxidative stress. Only small traces of: DMPO-OOH (superoxide) were detected in Brassica hypocotyl segments. In contrast, in DMPO-DMSO based extracts of Arabidopsis seedlings sprayed with diquat (0.1 %), superoxide radicals were detected as adducts of DMPO. Consequently, the non-detection of DMPO-OOH in leaves of untreated Arabidopsis seedlings and the very weak signals detected in Brassica napus hypocotyls are clearly related to the absence or the very small amounts of superoxide in both tissues.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
electron paramagnetic resonance, Brassica napus, free radical, spin-trapping, oxidative stress, FREE-RADICALS, SUPEROXIDE, SPECTROSCOPY, CHLOROPLASTS, OXYGEN
journal title
JOURNAL OF PLANT PHYSIOLOGY
J. Plant Physiol.
volume
154
issue
1
pages
132 - 136
Web of Science type
Article
Web of Science id
000078280200019
ISSN
0176-1617
DOI
10.1016/S0176-1617(99)80329-0
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
173740
handle
http://hdl.handle.net/1854/LU-173740
date created
2004-01-14 13:40:00
date last changed
2016-12-19 15:38:43
@article{173740,
  abstract     = {Oxygen derived radicals were measured in Brassica napus hypocotyl segments by means of EPR (electron paramagnetic resonance) after spin-trapping with 5,5-dimethyl-1-pyrroline-N-oxide (DMPO) in a dimethylsulfoxide(DMSO)-based medium. Next to the ascorbyl radical signal, an EPR signal due to DMPO-CH3, generated by reaction of free OH radicals with DMPO, was detected. The DMPO-CH3 signals were quantified in Brassica napus hypocotyl segments at different times after wounding and in vitro culture. A maximum was detected as a function of time after wounding. When hypocotyl segments were kept in the dark, a reduction of about 70\% of both the DMPO-CH3 and ascorbyl line intensity in comparison to the light control was observed. This suggests that the production of ascorbyl. and hydroxyl radicals is associated with photooxidative stress. Only small traces of: DMPO-OOH (superoxide) were detected in Brassica hypocotyl segments. In contrast, in DMPO-DMSO based extracts of Arabidopsis seedlings sprayed with diquat (0.1 \%), superoxide radicals were detected as adducts of DMPO. Consequently, the non-detection of DMPO-OOH in leaves of untreated Arabidopsis seedlings and the very weak signals detected in Brassica napus hypocotyls are clearly related to the absence or the very small amounts of superoxide in both tissues.},
  author       = {Van Doorslaer, Sabine and Dedonder, Andr{\'e}e and De Block, Marc and Callens, Freddy},
  issn         = {0176-1617},
  journal      = {JOURNAL OF PLANT PHYSIOLOGY},
  keyword      = {electron paramagnetic resonance,Brassica napus,free radical,spin-trapping,oxidative stress,FREE-RADICALS,SUPEROXIDE,SPECTROSCOPY,CHLOROPLASTS,OXYGEN},
  language     = {eng},
  number       = {1},
  pages        = {132--136},
  title        = {Oxidative stress in plants: EPR monitoring in DMPO-DMSO based extracts},
  url          = {http://dx.doi.org/10.1016/S0176-1617(99)80329-0},
  volume       = {154},
  year         = {1999},
}

Chicago
Van Doorslaer, Sabine, Andrée Dedonder, Marc De Block, and Freddy Callens. 1999. “Oxidative Stress in Plants: EPR Monitoring in DMPO-DMSO Based Extracts.” Journal of Plant Physiology 154 (1): 132–136.
APA
Van Doorslaer, S., Dedonder, A., De Block, M., & Callens, F. (1999). Oxidative stress in plants: EPR monitoring in DMPO-DMSO based extracts. JOURNAL OF PLANT PHYSIOLOGY, 154(1), 132–136.
Vancouver
1.
Van Doorslaer S, Dedonder A, De Block M, Callens F. Oxidative stress in plants: EPR monitoring in DMPO-DMSO based extracts. JOURNAL OF PLANT PHYSIOLOGY. 1999;154(1):132–6.
MLA
Van Doorslaer, Sabine, Andrée Dedonder, Marc De Block, et al. “Oxidative Stress in Plants: EPR Monitoring in DMPO-DMSO Based Extracts.” JOURNAL OF PLANT PHYSIOLOGY 154.1 (1999): 132–136. Print.