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Assessment of blood neutrophil oxidative burst activity in dairy cows during the period of parturition

(1998) ANIMAL SCIENCE. 67(3). p.421-426
Author
Organization
Abstract
Bovine blood neutrophil oxidative burst activity, the total leukocyte count and its differentiation, as well as progesterone, glucose and cortisol levels were studied on 10 cows from 1 week prior to calving until 8 weeks after parturition. From day -7 before parturition a decrease in the metabolic oxidative burst activity of neutrophils was observed, reaching the lowest value 3 days after calving (0.28 (s.e. 0.06) nmol/l H2O2 per min 10(6) neutrophils) (P less than or equal to 0.01). Thereafter, values increased with a maximum of activity 26 days after parturition (0.57 (s.e. 0.05) nmol/H2O2 per min per 10(6) neutrophils). At the end of the experiment the neutrophils H2O2-production was comparable to values observed on day -7. The maximum total leukocyte count was observed on the day of parturition (8,108 (s.e. 370) leukocytes per mu l blood) (P less than or equal to 0.01) returning to normal levels about 3 weeks after calving. Lymphocytes and neutrophils showed a trend analogous to that of the total leukocytes. Eosinophils and monocytes did not change during the entire period. With the rapid increase of circulating neutrophils observed on the calving day, a marked left shift was observed with a maximum number of immature neutrophils (48.6%) observed on day +3 (1477 (s.e. 242) immature neutrophils per mu l blood) (P less than or equal to 0.01), reaching normal values about 4 weeks after parturition. A significant increase (P less than or equal to 0.01) of plasma glucose was found on the day of parturition (91.4 (s.e. 6.8) mg per 100 ml plasma), while the levels of cortisol reached their maximum 3 clays after calving (22.05 (s.e. 8.46) ng/ml plasma). Progesterone levels averaged 4.07 (s.e. 1.21) mu g/l 7 days before calving, declining precipitously to 0.06 (s.e. 0.45) mu g/l 3 days after calving (P less than or equal to 0.01), remaining very low for a period of about 3 weeks. Minimum and maximum anoestrus post-partum were 12 and 57 days, respectively. Despite great hormonal variation observed in the peripartum period, no significant correlation was found between measured hormones and the oxidative burst activity of neutrophils.
Keywords
HOLSTEIN COWS, ESCHERICHIA-COLI MASTITIS, PHAGOCYTOSIS, LEUKOCYTES, ESTRADIOL, ASSOCIATION, CORTICOSTEROIDS, PROGESTERONE, PREGNANCY, LACTATION, parturition, cows, neutrophils

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MLA
Moreira da Silva, F, Christian Burvenich, Anna Leen, et al. “Assessment of Blood Neutrophil Oxidative Burst Activity in Dairy Cows During the Period of Parturition.” ANIMAL SCIENCE 67.3 (1998): 421–426. Print.
APA
Moreira da Silva, F, Burvenich, C., Leen, A., & Brossé, L. (1998). Assessment of blood neutrophil oxidative burst activity in dairy cows during the period of parturition. ANIMAL SCIENCE, 67(3), 421–426.
Chicago author-date
Moreira da Silva, F, Christian Burvenich, Anna Leen, and L Brossé. 1998. “Assessment of Blood Neutrophil Oxidative Burst Activity in Dairy Cows During the Period of Parturition.” Animal Science 67 (3): 421–426.
Chicago author-date (all authors)
Moreira da Silva, F, Christian Burvenich, Anna Leen, and L Brossé. 1998. “Assessment of Blood Neutrophil Oxidative Burst Activity in Dairy Cows During the Period of Parturition.” Animal Science 67 (3): 421–426.
Vancouver
1.
Moreira da Silva F, Burvenich C, Leen A, Brossé L. Assessment of blood neutrophil oxidative burst activity in dairy cows during the period of parturition. ANIMAL SCIENCE. 1998;67(3):421–6.
IEEE
[1]
F. Moreira da Silva, C. Burvenich, A. Leen, and L. Brossé, “Assessment of blood neutrophil oxidative burst activity in dairy cows during the period of parturition,” ANIMAL SCIENCE, vol. 67, no. 3, pp. 421–426, 1998.
@article{173164,
  abstract     = {Bovine blood neutrophil oxidative burst activity, the total leukocyte count and its differentiation, as well as progesterone, glucose and cortisol levels were studied on 10 cows from 1 week prior to calving until 8 weeks after parturition.
From day -7 before parturition a decrease in the metabolic oxidative burst activity of neutrophils was observed, reaching the lowest value 3 days after calving (0.28 (s.e. 0.06) nmol/l H2O2 per min 10(6) neutrophils) (P less than or equal to 0.01). Thereafter, values increased with a maximum of activity 26 days after parturition (0.57 (s.e. 0.05) nmol/H2O2 per min per 10(6) neutrophils). At the end of the experiment the neutrophils H2O2-production was comparable to values observed on day -7.
The maximum total leukocyte count was observed on the day of parturition (8,108 (s.e. 370) leukocytes per mu l blood) (P less than or equal to 0.01) returning to normal levels about 3 weeks after calving. Lymphocytes and neutrophils showed a trend analogous to that of the total leukocytes. Eosinophils and monocytes did not change during the entire period. With the rapid increase of circulating neutrophils observed on the calving day, a marked left shift was observed with a maximum number of immature neutrophils (48.6%) observed on day +3 (1477 (s.e. 242) immature neutrophils per mu l blood) (P less than or equal to 0.01), reaching normal values about 4 weeks after parturition. A significant increase (P less than or equal to 0.01) of plasma glucose was found on the day of parturition (91.4 (s.e. 6.8) mg per 100 ml plasma), while the levels of cortisol reached their maximum 3 clays after calving (22.05 (s.e. 8.46) ng/ml plasma).
Progesterone levels averaged 4.07 (s.e. 1.21) mu g/l 7 days before calving, declining precipitously to 0.06 (s.e. 0.45) mu g/l 3 days after calving (P less than or equal to 0.01), remaining very low for a period of about 3 weeks. Minimum and maximum anoestrus post-partum were 12 and 57 days, respectively. Despite great hormonal variation observed in the peripartum period, no significant correlation was found between measured hormones and the oxidative burst activity of neutrophils.},
  author       = {Moreira da Silva, F and Burvenich, Christian and Leen, Anna and Brossé, L},
  issn         = {1357-7298},
  journal      = {ANIMAL SCIENCE},
  keywords     = {HOLSTEIN COWS,ESCHERICHIA-COLI MASTITIS,PHAGOCYTOSIS,LEUKOCYTES,ESTRADIOL,ASSOCIATION,CORTICOSTEROIDS,PROGESTERONE,PREGNANCY,LACTATION,parturition,cows,neutrophils},
  language     = {eng},
  number       = {3},
  pages        = {421--426},
  title        = {Assessment of blood neutrophil oxidative burst activity in dairy cows during the period of parturition},
  volume       = {67},
  year         = {1998},
}

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