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Abstract
Amplified fragment length polymorphism (AFLP) is a high-throughput, molecular-marker technique that is used increasingly in a variety of genetic analyses. Here, the conditions for carrying out AFLP analysis have been established for different tree species, including both angiosperm and gymnosperm trees, with genome sizes ranging from 0.54 to 38 pg DNA/2C. Specific parameters have been determined to provide informative and reproducible AFLP fingerprints of peach (Prunus persica L.), eucalypt, oak, poplar, and loblolly pine (Pinus taeda L.). Typically, 80-130 amplified DNA fragments (i.e., loci analyzed per primer combination) were obtained. Subsequently, these AFLP conditions were evaluated for intra- and inter-specific genetic variability studies as well as for genome mapping purposes of woody species. This work demonstrates that AFLP is a powerful tool in forest tree genetics.
Keywords
POPULUS, PINUS, GENOME, NUCLEAR-DNA CONTENT, MOLECULAR MARKERS, GENETIC-MAP, LINKAGE MAPS, RAPD MARKERS, FRAGMENT LENGTH POLYMORPHISMS, EUCALYPTUS-UROPHYLLA

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Citation

Please use this url to cite or link to this publication:

Chicago
Cervera, María Teresa, D Remington, J-M Frigerio, Veronique Storme, Bart Ivens, Wout Boerjan, and C Plomion. 2000. “Improved AFLP Analysis of Tree Species.” Canadian Journal of Forest Research-revue Canadienne De Recherche Forestiere 30 (10): 1608–1616.
APA
Cervera, María Teresa, Remington, D., Frigerio, J.-M., Storme, V., Ivens, B., Boerjan, W., & Plomion, C. (2000). Improved AFLP analysis of tree species. CANADIAN JOURNAL OF FOREST RESEARCH-REVUE CANADIENNE DE RECHERCHE FORESTIERE, 30(10), 1608–1616.
Vancouver
1.
Cervera MT, Remington D, Frigerio J-M, Storme V, Ivens B, Boerjan W, et al. Improved AFLP analysis of tree species. CANADIAN JOURNAL OF FOREST RESEARCH-REVUE CANADIENNE DE RECHERCHE FORESTIERE. 2000;30(10):1608–16.
MLA
Cervera, María Teresa, D Remington, J-M Frigerio, et al. “Improved AFLP Analysis of Tree Species.” CANADIAN JOURNAL OF FOREST RESEARCH-REVUE CANADIENNE DE RECHERCHE FORESTIERE 30.10 (2000): 1608–1616. Print.
@article{172817,
  abstract     = {Amplified fragment length polymorphism (AFLP) is a high-throughput, molecular-marker technique that is used increasingly in a variety of genetic analyses. Here, the conditions for carrying out AFLP analysis have been established for different tree species, including both angiosperm and gymnosperm trees, with genome sizes ranging from 0.54 to 38 pg DNA/2C. Specific parameters have been determined to provide informative and reproducible AFLP fingerprints of peach (Prunus persica L.), eucalypt, oak, poplar, and loblolly pine (Pinus taeda L.). Typically, 80-130 amplified DNA fragments (i.e., loci analyzed per primer combination) were obtained. Subsequently, these AFLP conditions were evaluated for intra- and inter-specific genetic variability studies as well as for genome mapping purposes of woody species. This work demonstrates that AFLP is a powerful tool in forest tree genetics.},
  author       = {Cervera, Mar{\'i}a Teresa and Remington, D and Frigerio, J-M and Storme, Veronique and Ivens, Bart and Boerjan, Wout and Plomion, C},
  issn         = {0045-5067},
  journal      = {CANADIAN JOURNAL OF FOREST RESEARCH-REVUE CANADIENNE DE RECHERCHE FORESTIERE},
  keyword      = {POPULUS,PINUS,GENOME,NUCLEAR-DNA CONTENT,MOLECULAR MARKERS,GENETIC-MAP,LINKAGE MAPS,RAPD MARKERS,FRAGMENT LENGTH POLYMORPHISMS,EUCALYPTUS-UROPHYLLA},
  language     = {eng},
  number       = {10},
  pages        = {1608--1616},
  title        = {Improved AFLP analysis of tree species},
  url          = {http://dx.doi.org/10.1139/cjfr-30-10-1608},
  volume       = {30},
  year         = {2000},
}

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