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Human cartilage gp-39+, CD16+monocytes in peripheral blood and synovium: correlation with joint destruction in rheumatoid arthritis

Dominique Baeten UGent, Annemieke MH Boots, Peter GA Steenbakkers, Dirk Elewaut UGent, Ebo Bos, Gijs FM Verheijden, August Verbruggen UGent, André MM Miltenburg, Anthonius WM Rijnders, Eric Veys, et al. (2000) ARTHRITIS AND RHEUMATISM. 43(6). p.1233-1243
abstract
Objective. To investigate the expression of human cartilage (HC) gp-39, a possible autoantigen in rheumatoid arthritis (RA), in peripheral brood and synovium, to characterize its cellular source, and to analyze correlations with clinical features, Methods. The expression of HC gp-39 in synovium and peripheral blood mononuclear cells (PBMC) was assessed by immunohistochemistry and flow cytometry, Synthesis and secretion were investigated by both reverse transcription-polymerase chain reaction and enzyme-linked immunosorbent assay. Results. PBRIC expressing HC gp-39 were increased in RA patients compared with spondylarthropathy patients (P = 0.0029) and with healthy control subjects (P = 0.0013), HC gp-39+ cells were also slightly overrepresented in RA synovium (P = 0.01). In both blood and synovium, HC gp-39+ cells were identified as CDT4dim, CD16+ monocytes, a phenotype which can differentiate from classic CD14++ monocytes by maturation in vitro. HC gp-39 messenger RNA was detected in RA synovium and PBMC, and PBMC secreted HC gp-39 in vitro. The number of HC gp-39+ PBMC correlated with serum levels of C-reactive protein (r = 0.39, P = 0.003) and HC gp-39 (r = 0.52, P = 0.014). HC gp-39 expression in Ri synovial lining correlated with joint destruction (r = 0.77, P < 0.001), Conclusion, CD16+ monocytes, a cellular source of HC gp-39 in vivo, are overrepresented in both RA peripheral blood and synovial tissue. The presence of HC gp-39+ cells in RA synovium is correlated with the degree of joint destruction. These data support a role of these cells in the local autoimmune response that leads to chronic inflammation and joint destruction.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
CHITINASE PROTEIN FAMILY, COLONY-STIMULATING FACTOR, TISSUE MACROPHAGES, MAMMALIAN MEMBER, MONOCYTES, YKL-40, CELLS, EXPRESSION, SUBSET, GLYCOPROTEIN-39
journal title
ARTHRITIS AND RHEUMATISM
Arthritis Rheum.
volume
43
issue
6
pages
1233 - 1243
Web of Science type
Article
Web of Science id
000087503300006
ISSN
0004-3591
DOI
10.1002/1529-0131(200006)43:6<1233::AID-ANR6>3.0.CO;2-9
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
171577
handle
http://hdl.handle.net/1854/LU-171577
date created
2004-01-14 13:40:00
date last changed
2016-12-19 15:37:55
@article{171577,
  abstract     = {Objective. To investigate the expression of human cartilage (HC) gp-39, a possible autoantigen in rheumatoid arthritis (RA), in peripheral brood and synovium, to characterize its cellular source, and to analyze correlations with clinical features, 
Methods. The expression of HC gp-39 in synovium and peripheral blood mononuclear cells (PBMC) was assessed by immunohistochemistry and flow cytometry, Synthesis and secretion were investigated by both reverse transcription-polymerase chain reaction and enzyme-linked immunosorbent assay. 
Results. PBRIC expressing HC gp-39 were increased in RA patients compared with spondylarthropathy patients (P = 0.0029) and with healthy control subjects (P = 0.0013), HC gp-39+ cells were also slightly overrepresented in RA synovium (P = 0.01). In both blood and synovium, HC gp-39+ cells were identified as CDT4dim, CD16+ monocytes, a phenotype which can differentiate from classic CD14++ monocytes by maturation in vitro. HC gp-39 messenger RNA was detected in RA synovium and PBMC, and PBMC secreted HC gp-39 in vitro. The number of HC gp-39+ PBMC correlated with serum levels of C-reactive protein (r = 0.39, P = 0.003) and HC gp-39 (r = 0.52, P = 0.014). HC gp-39 expression in Ri synovial lining correlated with joint destruction (r = 0.77, P {\textlangle} 0.001), 
Conclusion, CD16+ monocytes, a cellular source of HC gp-39 in vivo, are overrepresented in both RA peripheral blood and synovial tissue. The presence of HC gp-39+ cells in RA synovium is correlated with the degree of joint destruction. These data support a role of these cells in the local autoimmune response that leads to chronic inflammation and joint destruction.},
  author       = {Baeten, Dominique and Boots, Annemieke MH and Steenbakkers, Peter GA and Elewaut, Dirk and Bos, Ebo and Verheijden, Gijs FM and Verbruggen, August and Miltenburg, Andr{\'e} MM and Rijnders, Anthonius WM and Veys, Eric and De Keyser, Filip},
  issn         = {0004-3591},
  journal      = {ARTHRITIS AND RHEUMATISM},
  keyword      = {CHITINASE PROTEIN FAMILY,COLONY-STIMULATING FACTOR,TISSUE MACROPHAGES,MAMMALIAN MEMBER,MONOCYTES,YKL-40,CELLS,EXPRESSION,SUBSET,GLYCOPROTEIN-39},
  language     = {eng},
  number       = {6},
  pages        = {1233--1243},
  title        = {Human cartilage gp-39+, CD16+monocytes in peripheral blood and synovium: correlation with joint destruction in rheumatoid arthritis},
  url          = {http://dx.doi.org/10.1002/1529-0131(200006)43:6{\textlangle}1233::AID-ANR6{\textrangle}3.0.CO;2-9},
  volume       = {43},
  year         = {2000},
}

Chicago
Baeten, Dominique, Annemieke MH Boots, Peter GA Steenbakkers, Dirk Elewaut, Ebo Bos, Gijs FM Verheijden, August Verbruggen, et al. 2000. “Human Cartilage Gp-39+, CD16+monocytes in Peripheral Blood and Synovium: Correlation with Joint Destruction in Rheumatoid Arthritis.” Arthritis and Rheumatism 43 (6): 1233–1243.
APA
Baeten, D., Boots, A. M., Steenbakkers, P. G., Elewaut, D., Bos, E., Verheijden, G. F., Verbruggen, A., et al. (2000). Human cartilage gp-39+, CD16+monocytes in peripheral blood and synovium: correlation with joint destruction in rheumatoid arthritis. ARTHRITIS AND RHEUMATISM, 43(6), 1233–1243.
Vancouver
1.
Baeten D, Boots AM, Steenbakkers PG, Elewaut D, Bos E, Verheijden GF, et al. Human cartilage gp-39+, CD16+monocytes in peripheral blood and synovium: correlation with joint destruction in rheumatoid arthritis. ARTHRITIS AND RHEUMATISM. 2000;43(6):1233–43.
MLA
Baeten, Dominique, Annemieke MH Boots, Peter GA Steenbakkers, et al. “Human Cartilage Gp-39+, CD16+monocytes in Peripheral Blood and Synovium: Correlation with Joint Destruction in Rheumatoid Arthritis.” ARTHRITIS AND RHEUMATISM 43.6 (2000): 1233–1243. Print.