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Broadening of the T cell receptor spectrum among rheumatoid arthritis synovial cell-lines in relation to disease duration

Dirk Elewaut (UGent), Filip De Keyser (UGent), Filip Van den Bosch (UGent), August Verbruggen (UGent) and Eric Veys (UGent)
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Abstract
Objective : The aim of the study was to evaluate the T cell receptor (TCR) family usage in T cell-lines from subcutaneous nodules and synovium from patients with rheumatoid arthritis (RA), with specific reference to the duration of symptoms. In vitro adherence characteristics of nodular T cells was studied as well. Methods : Monoclonal antibodies were used to determine the distribution of TCR families in T cell-lines from synovium of patients with early and long-standing RA, from rheumatoid nodules and control tissues. An in vitro binding assay with T cell-lines from 2 rheumatoid nodules was performed. Results : In early RA synovium, a restricted TCR family usage was observed in 5 out of 8 patients, contrary to long-standing disease, peripheral blood ileum and colon. In RA nodules, a similar degree of restriction was noted. Moreover; the same TCR family was overexpressed by T cell-lines from different nodules derived from the same patient. T cell-lines from rheumatoid nodules demonstrated a preferential in vitro adherence to rheumatoid synovium and rheumatoid nodules, while no binding was observed on skin or tonsil. Conclusion : The TCR spectrum among RA synovial cell-fines broadens in relation to the disease duration. The overexpression of the same TCR family in different rheumatoid nodules from the same patients, and the in vitro adherence of T cell-lines from rheumatoid nodules may be indicative for recirculation between the different disease manifestations in RA.
Keywords
rheumatoid arthritis, T cell receptor, rheumatoid nodules, rheumatoid synovium, MONOCLONAL-ANTIBODIES, EXPANDED LYMPHOCYTES, NODULES, FLUID, REPERTOIRE, EXPANSION, MEMBRANES, USAGE, BLOOD, ALPHA

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Chicago
Elewaut, Dirk, Filip De Keyser, Filip Van den Bosch, August Verbruggen, and Eric Veys. 2000. “Broadening of the T Cell Receptor Spectrum Among Rheumatoid Arthritis Synovial Cell-lines in Relation to Disease Duration.” Clinical and Experimental Rheumatology 18 (2): 201–207.
APA
Elewaut, D., De Keyser, F., Van den Bosch, F., Verbruggen, A., & Veys, E. (2000). Broadening of the T cell receptor spectrum among rheumatoid arthritis synovial cell-lines in relation to disease duration. CLINICAL AND EXPERIMENTAL RHEUMATOLOGY, 18(2), 201–207.
Vancouver
1.
Elewaut D, De Keyser F, Van den Bosch F, Verbruggen A, Veys E. Broadening of the T cell receptor spectrum among rheumatoid arthritis synovial cell-lines in relation to disease duration. CLINICAL AND EXPERIMENTAL RHEUMATOLOGY. 2000;18(2):201–7.
MLA
Elewaut, Dirk, Filip De Keyser, Filip Van den Bosch, et al. “Broadening of the T Cell Receptor Spectrum Among Rheumatoid Arthritis Synovial Cell-lines in Relation to Disease Duration.” CLINICAL AND EXPERIMENTAL RHEUMATOLOGY 18.2 (2000): 201–207. Print.
@article{171030,
  abstract     = {Objective : The aim of the study was to evaluate the T cell receptor (TCR) family usage in T cell-lines from subcutaneous nodules and synovium from patients with rheumatoid arthritis (RA), with specific reference to the duration of symptoms. In vitro adherence characteristics of nodular T cells was studied as well. 
Methods : Monoclonal antibodies were used to determine the distribution of TCR families in T cell-lines from synovium of patients with early and long-standing RA, from rheumatoid nodules and control tissues. An in vitro binding assay with T cell-lines from 2 rheumatoid nodules was performed. 
Results : In early RA synovium, a restricted TCR family usage was observed in 5 out of 8 patients, contrary to long-standing disease, peripheral blood ileum and colon. In RA nodules, a similar degree of restriction was noted. Moreover; the same TCR family was overexpressed by T cell-lines from different nodules derived from the same patient. T cell-lines from rheumatoid nodules demonstrated a preferential in vitro adherence to rheumatoid synovium and rheumatoid nodules, while no binding was observed on skin or tonsil. 
Conclusion : The TCR spectrum among RA synovial cell-fines broadens in relation to the disease duration. The overexpression of the same TCR family in different rheumatoid nodules from the same patients, and the in vitro adherence of T cell-lines from rheumatoid nodules may be indicative for recirculation between the different disease manifestations in RA.},
  author       = {Elewaut, Dirk and De Keyser, Filip and Van den Bosch, Filip and Verbruggen, August and Veys, Eric},
  issn         = {0392-856X},
  journal      = {CLINICAL AND EXPERIMENTAL RHEUMATOLOGY},
  keyword      = {rheumatoid arthritis,T cell receptor,rheumatoid nodules,rheumatoid synovium,MONOCLONAL-ANTIBODIES,EXPANDED LYMPHOCYTES,NODULES,FLUID,REPERTOIRE,EXPANSION,MEMBRANES,USAGE,BLOOD,ALPHA},
  language     = {eng},
  number       = {2},
  pages        = {201--207},
  title        = {Broadening of the T cell receptor spectrum among rheumatoid arthritis synovial cell-lines in relation to disease duration},
  url          = {http://www.clinexprheumatol.org/abstract.asp?a=1580},
  volume       = {18},
  year         = {2000},
}

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