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Broadening of the T cell receptor spectrum among rheumatoid arthritis synovial cell-lines in relation to disease duration

Dirk Elewaut UGent, Filip De Keyser UGent, Filip Van den Bosch UGent, August Verbruggen UGent and Eric Veys (2000) CLINICAL AND EXPERIMENTAL RHEUMATOLOGY. 18(2). p.201-207
abstract
Objective : The aim of the study was to evaluate the T cell receptor (TCR) family usage in T cell-lines from subcutaneous nodules and synovium from patients with rheumatoid arthritis (RA), with specific reference to the duration of symptoms. In vitro adherence characteristics of nodular T cells was studied as well. Methods : Monoclonal antibodies were used to determine the distribution of TCR families in T cell-lines from synovium of patients with early and long-standing RA, from rheumatoid nodules and control tissues. An in vitro binding assay with T cell-lines from 2 rheumatoid nodules was performed. Results : In early RA synovium, a restricted TCR family usage was observed in 5 out of 8 patients, contrary to long-standing disease, peripheral blood ileum and colon. In RA nodules, a similar degree of restriction was noted. Moreover; the same TCR family was overexpressed by T cell-lines from different nodules derived from the same patient. T cell-lines from rheumatoid nodules demonstrated a preferential in vitro adherence to rheumatoid synovium and rheumatoid nodules, while no binding was observed on skin or tonsil. Conclusion : The TCR spectrum among RA synovial cell-fines broadens in relation to the disease duration. The overexpression of the same TCR family in different rheumatoid nodules from the same patients, and the in vitro adherence of T cell-lines from rheumatoid nodules may be indicative for recirculation between the different disease manifestations in RA.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
rheumatoid arthritis, T cell receptor, rheumatoid nodules, rheumatoid synovium, MONOCLONAL-ANTIBODIES, EXPANDED LYMPHOCYTES, NODULES, FLUID, REPERTOIRE, EXPANSION, MEMBRANES, USAGE, BLOOD, ALPHA
journal title
CLINICAL AND EXPERIMENTAL RHEUMATOLOGY
Clin. Exp. Rheumatol.
volume
18
issue
2
pages
201 - 207
Web of Science type
Article
Web of Science id
000086420400005
ISSN
0392-856X
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
171030
handle
http://hdl.handle.net/1854/LU-171030
alternative location
http://www.clinexprheumatol.org/abstract.asp?a=1580
date created
2004-01-14 13:40:00
date last changed
2016-12-19 15:37:55
@article{171030,
  abstract     = {Objective : The aim of the study was to evaluate the T cell receptor (TCR) family usage in T cell-lines from subcutaneous nodules and synovium from patients with rheumatoid arthritis (RA), with specific reference to the duration of symptoms. In vitro adherence characteristics of nodular T cells was studied as well. 
Methods : Monoclonal antibodies were used to determine the distribution of TCR families in T cell-lines from synovium of patients with early and long-standing RA, from rheumatoid nodules and control tissues. An in vitro binding assay with T cell-lines from 2 rheumatoid nodules was performed. 
Results : In early RA synovium, a restricted TCR family usage was observed in 5 out of 8 patients, contrary to long-standing disease, peripheral blood ileum and colon. In RA nodules, a similar degree of restriction was noted. Moreover; the same TCR family was overexpressed by T cell-lines from different nodules derived from the same patient. T cell-lines from rheumatoid nodules demonstrated a preferential in vitro adherence to rheumatoid synovium and rheumatoid nodules, while no binding was observed on skin or tonsil. 
Conclusion : The TCR spectrum among RA synovial cell-fines broadens in relation to the disease duration. The overexpression of the same TCR family in different rheumatoid nodules from the same patients, and the in vitro adherence of T cell-lines from rheumatoid nodules may be indicative for recirculation between the different disease manifestations in RA.},
  author       = {Elewaut, Dirk and De Keyser, Filip and Van den Bosch, Filip and Verbruggen, August and Veys, Eric},
  issn         = {0392-856X},
  journal      = {CLINICAL AND EXPERIMENTAL RHEUMATOLOGY},
  keyword      = {rheumatoid arthritis,T cell receptor,rheumatoid nodules,rheumatoid synovium,MONOCLONAL-ANTIBODIES,EXPANDED LYMPHOCYTES,NODULES,FLUID,REPERTOIRE,EXPANSION,MEMBRANES,USAGE,BLOOD,ALPHA},
  language     = {eng},
  number       = {2},
  pages        = {201--207},
  title        = {Broadening of the T cell receptor spectrum among rheumatoid arthritis synovial cell-lines in relation to disease duration},
  url          = {http://www.clinexprheumatol.org/abstract.asp?a=1580},
  volume       = {18},
  year         = {2000},
}

Chicago
Elewaut, Dirk, Filip De Keyser, Filip Van den Bosch, August Verbruggen, and Eric Veys. 2000. “Broadening of the T Cell Receptor Spectrum Among Rheumatoid Arthritis Synovial Cell-lines in Relation to Disease Duration.” Clinical and Experimental Rheumatology 18 (2): 201–207.
APA
Elewaut, D., De Keyser, F., Van den Bosch, F., Verbruggen, A., & Veys, E. (2000). Broadening of the T cell receptor spectrum among rheumatoid arthritis synovial cell-lines in relation to disease duration. CLINICAL AND EXPERIMENTAL RHEUMATOLOGY, 18(2), 201–207.
Vancouver
1.
Elewaut D, De Keyser F, Van den Bosch F, Verbruggen A, Veys E. Broadening of the T cell receptor spectrum among rheumatoid arthritis synovial cell-lines in relation to disease duration. CLINICAL AND EXPERIMENTAL RHEUMATOLOGY. 2000;18(2):201–7.
MLA
Elewaut, Dirk, Filip De Keyser, Filip Van den Bosch, et al. “Broadening of the T Cell Receptor Spectrum Among Rheumatoid Arthritis Synovial Cell-lines in Relation to Disease Duration.” CLINICAL AND EXPERIMENTAL RHEUMATOLOGY 18.2 (2000): 201–207. Print.