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Srchi24, a chitinase homolog lacking an essential glutamic acid residue for hydrolytic activity, is induced during nodule development on Sesbania rostrata

(2001) PLANT PHYSIOLOGY. 127(1). p.78-89
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Abstract
The interaction between the tropical legume Sesbania rostrata and the bacterium Azorhizobium caulinodans results in the formation of nodules on both stem and roots. Stem nodulation was used as a model system to isolate early markers differential display. One of them, Srchi24 is a novel early nodulin whose transcript level increased already 4 h after inoculation. This enhancement depended on Nod factor-producing bacteria. Srchi24 transcript levels were induced also by exogenous cytokinins. In situ hybridization and immunolocalization experiments showed that Srchi24 transcripts and proteins were present in the outermost cortical cell layers of the developing nodules. Sequence analyses revealed that Srchi24 is similar to class III chitinases, but lacks an important catalytic glutamate residue. A fusion between a maltose-binding protein and Srchi24 had no detectable hydrolytic activity. A function in nodulation is proposed for the Srchi24 protein.
Keywords
PLANT CHITINASES, GLYCOSYL HYDROLASES, NODULATION FACTORS, EXPRESSION PATTERNS, AZORHIZOBIUM-CAULINODANS, MAX L. MERR., AFFINITY BINDING-SITE, CELL-SUSPENSION CULTURES, OLIGOSACCHARIDIC NODRM FACTORS, PATHOGENESIS-RELATED PROTEINS

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Citation

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Chicago
Goormachtig, Sofie, Willem Van De Velde, Sam Lievens, Christa Verplancke, Sylvia Herman, Annick De Keyser, and Marcella Holsters. 2001. “Srchi24, a Chitinase Homolog Lacking an Essential Glutamic Acid Residue for Hydrolytic Activity, Is Induced During Nodule Development on Sesbania Rostrata.” Plant Physiology 127 (1): 78–89.
APA
Goormachtig, S., Van De Velde, W., Lievens, S., Verplancke, C., Herman, S., De Keyser, A., & Holsters, M. (2001). Srchi24, a chitinase homolog lacking an essential glutamic acid residue for hydrolytic activity, is induced during nodule development on Sesbania rostrata. PLANT PHYSIOLOGY, 127(1), 78–89.
Vancouver
1.
Goormachtig S, Van De Velde W, Lievens S, Verplancke C, Herman S, De Keyser A, et al. Srchi24, a chitinase homolog lacking an essential glutamic acid residue for hydrolytic activity, is induced during nodule development on Sesbania rostrata. PLANT PHYSIOLOGY. 2001;127(1):78–89.
MLA
Goormachtig, Sofie, Willem Van De Velde, Sam Lievens, et al. “Srchi24, a Chitinase Homolog Lacking an Essential Glutamic Acid Residue for Hydrolytic Activity, Is Induced During Nodule Development on Sesbania Rostrata.” PLANT PHYSIOLOGY 127.1 (2001): 78–89. Print.
@article{169707,
  abstract     = {The interaction between the tropical legume Sesbania rostrata and the bacterium Azorhizobium caulinodans results in the formation of nodules on both stem and roots. Stem nodulation was used as a model system to isolate early markers differential display. One of them, Srchi24 is a novel early nodulin whose transcript level increased already 4 h after inoculation. This enhancement depended on Nod factor-producing bacteria. Srchi24 transcript levels were induced also by exogenous cytokinins. In situ hybridization and immunolocalization experiments showed that Srchi24 transcripts and proteins were present in the outermost cortical cell layers of the developing nodules. Sequence analyses revealed that Srchi24 is similar to class III chitinases, but lacks an important catalytic glutamate residue. A fusion between a maltose-binding protein and Srchi24 had no detectable hydrolytic activity. A function in nodulation is proposed for the Srchi24 protein.},
  author       = {Goormachtig, Sofie and Van De Velde, Willem and Lievens, Sam and Verplancke, Christa and Herman, Sylvia and De Keyser, Annick and Holsters, Marcella},
  issn         = {0032-0889},
  journal      = {PLANT PHYSIOLOGY},
  keyword      = {PLANT CHITINASES,GLYCOSYL HYDROLASES,NODULATION FACTORS,EXPRESSION PATTERNS,AZORHIZOBIUM-CAULINODANS,MAX L. MERR.,AFFINITY BINDING-SITE,CELL-SUSPENSION CULTURES,OLIGOSACCHARIDIC NODRM FACTORS,PATHOGENESIS-RELATED PROTEINS},
  language     = {eng},
  number       = {1},
  pages        = {78--89},
  title        = {Srchi24, a chitinase homolog lacking an essential glutamic acid residue for hydrolytic activity, is induced during nodule development on Sesbania rostrata},
  url          = {http://dx.doi.org/10.1104/pp.127.1.78},
  volume       = {127},
  year         = {2001},
}

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