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Bovine melanocortin receptor 4: cDNA sequence, polymorphisms and mapping

Andy Haegeman, Frank Coopman UGent, K Jacobs, M Mattheews, Alex Van Zeveren UGent and Luc Peelman UGent (2001) ANIMAL GENETICS. 32(4). p.189-192
abstract
A cDNA encoding the bovine melanocortin receptor 4 (MC4R) was cloned and sequenced. Comparing human, pig and rat homologues showed a 87, 85 and 89% identity on the DNA level, respectively, and over 90% on the protein level. The bovine MC4R gene was mapped to BTU 24 by radiation hybrid mapping. Two nucleotide changes were identified by single stranded conformation polymorphism (SSCP) and sequencing. The substitutions proved to be a T to C and G (allele B) to A (allele A) resulting, respectively, in a conservative valine to alanine substitution (Val 145 Ala) and an alanine to threonine (Ala 172 Thr). Using PCR-RFLP, 13 different cattle breeds were screened for the presence of the Ala 172 Thr substitution. With the exception of one Red Pied animal, allele A could only be detected in Red Holstein animals.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
melanocortin receptor 4, cDNA, mutation, PCR, RFLP, SSCP, BODY-WEIGHT REGULATION, OBESE GENE-PRODUCT, LEPTIN PATHWAYS, OB/OB MICE, RADIATION
journal title
ANIMAL GENETICS
Anim. Genet.
volume
32
issue
4
pages
189 - 192
Web of Science type
Article
Web of Science id
000171020200002
JCR category
AGRICULTURE, DAIRY & ANIMAL SCIENCE
JCR impact factor
1.02 (2001)
JCR rank
12/43 (2001)
JCR quartile
2 (2001)
ISSN
0268-9146
DOI
10.1046/j.1365-2052.2001.00750.x
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
169628
handle
http://hdl.handle.net/1854/LU-169628
date created
2004-01-14 13:40:00
date last changed
2016-12-19 15:37:55
@article{169628,
  abstract     = {A cDNA encoding the bovine melanocortin receptor 4 (MC4R) was cloned and sequenced. Comparing human, pig and rat homologues showed a 87, 85 and 89\% identity on the DNA level, respectively, and over 90\% on the protein level. The bovine MC4R gene was mapped to BTU 24 by radiation hybrid mapping. Two nucleotide changes were identified by single stranded conformation polymorphism (SSCP) and sequencing. The substitutions proved to be a T to C and G (allele B) to A (allele A) resulting, respectively, in a conservative valine to alanine substitution (Val 145 Ala) and an alanine to threonine (Ala 172 Thr). Using PCR-RFLP, 13 different cattle breeds were screened for the presence of the Ala 172 Thr substitution. With the exception of one Red Pied animal, allele A could only be detected in Red Holstein animals.},
  author       = {Haegeman, Andy and Coopman, Frank and Jacobs, K and Mattheews, M and Van Zeveren, Alex and Peelman, Luc},
  issn         = {0268-9146},
  journal      = {ANIMAL GENETICS},
  keyword      = {melanocortin receptor 4,cDNA,mutation,PCR,RFLP,SSCP,BODY-WEIGHT REGULATION,OBESE GENE-PRODUCT,LEPTIN PATHWAYS,OB/OB MICE,RADIATION},
  language     = {eng},
  number       = {4},
  pages        = {189--192},
  title        = {Bovine melanocortin receptor 4: cDNA sequence, polymorphisms and mapping},
  url          = {http://dx.doi.org/10.1046/j.1365-2052.2001.00750.x},
  volume       = {32},
  year         = {2001},
}

Chicago
Haegeman, Andy, Frank Coopman, K Jacobs, M Mattheews, Alex Van Zeveren, and Luc Peelman. 2001. “Bovine Melanocortin Receptor 4: cDNA Sequence, Polymorphisms and Mapping.” Animal Genetics 32 (4): 189–192.
APA
Haegeman, Andy, Coopman, F., Jacobs, K., Mattheews, M., Van Zeveren, A., & Peelman, L. (2001). Bovine melanocortin receptor 4: cDNA sequence, polymorphisms and mapping. ANIMAL GENETICS, 32(4), 189–192.
Vancouver
1.
Haegeman A, Coopman F, Jacobs K, Mattheews M, Van Zeveren A, Peelman L. Bovine melanocortin receptor 4: cDNA sequence, polymorphisms and mapping. ANIMAL GENETICS. 2001;32(4):189–92.
MLA
Haegeman, Andy, Frank Coopman, K Jacobs, et al. “Bovine Melanocortin Receptor 4: cDNA Sequence, Polymorphisms and Mapping.” ANIMAL GENETICS 32.4 (2001): 189–192. Print.