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Bovine melanocortin receptor 4: cDNA sequence, polymorphisms and mapping

(2001) ANIMAL GENETICS. 32(4). p.189-192
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Abstract
A cDNA encoding the bovine melanocortin receptor 4 (MC4R) was cloned and sequenced. Comparing human, pig and rat homologues showed a 87, 85 and 89% identity on the DNA level, respectively, and over 90% on the protein level. The bovine MC4R gene was mapped to BTU 24 by radiation hybrid mapping. Two nucleotide changes were identified by single stranded conformation polymorphism (SSCP) and sequencing. The substitutions proved to be a T to C and G (allele B) to A (allele A) resulting, respectively, in a conservative valine to alanine substitution (Val 145 Ala) and an alanine to threonine (Ala 172 Thr). Using PCR-RFLP, 13 different cattle breeds were screened for the presence of the Ala 172 Thr substitution. With the exception of one Red Pied animal, allele A could only be detected in Red Holstein animals.
Keywords
melanocortin receptor 4, cDNA, mutation, PCR, RFLP, SSCP, BODY-WEIGHT REGULATION, OBESE GENE-PRODUCT, LEPTIN PATHWAYS, OB/OB MICE, RADIATION

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Citation

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Chicago
Haegeman, Andy, Frank Coopman, K Jacobs, M Mattheews, Alex Van Zeveren, and Luc Peelman. 2001. “Bovine Melanocortin Receptor 4: cDNA Sequence, Polymorphisms and Mapping.” Animal Genetics 32 (4): 189–192.
APA
Haegeman, Andy, Coopman, F., Jacobs, K., Mattheews, M., Van Zeveren, A., & Peelman, L. (2001). Bovine melanocortin receptor 4: cDNA sequence, polymorphisms and mapping. ANIMAL GENETICS, 32(4), 189–192.
Vancouver
1.
Haegeman A, Coopman F, Jacobs K, Mattheews M, Van Zeveren A, Peelman L. Bovine melanocortin receptor 4: cDNA sequence, polymorphisms and mapping. ANIMAL GENETICS. 2001;32(4):189–92.
MLA
Haegeman, Andy, Frank Coopman, K Jacobs, et al. “Bovine Melanocortin Receptor 4: cDNA Sequence, Polymorphisms and Mapping.” ANIMAL GENETICS 32.4 (2001): 189–192. Print.
@article{169628,
  abstract     = {A cDNA encoding the bovine melanocortin receptor 4 (MC4R) was cloned and sequenced. Comparing human, pig and rat homologues showed a 87, 85 and 89\% identity on the DNA level, respectively, and over 90\% on the protein level. The bovine MC4R gene was mapped to BTU 24 by radiation hybrid mapping. Two nucleotide changes were identified by single stranded conformation polymorphism (SSCP) and sequencing. The substitutions proved to be a T to C and G (allele B) to A (allele A) resulting, respectively, in a conservative valine to alanine substitution (Val 145 Ala) and an alanine to threonine (Ala 172 Thr). Using PCR-RFLP, 13 different cattle breeds were screened for the presence of the Ala 172 Thr substitution. With the exception of one Red Pied animal, allele A could only be detected in Red Holstein animals.},
  author       = {Haegeman, Andy and Coopman, Frank and Jacobs, K and Mattheews, M and Van Zeveren, Alex and Peelman, Luc},
  issn         = {0268-9146},
  journal      = {ANIMAL GENETICS},
  keyword      = {melanocortin receptor 4,cDNA,mutation,PCR,RFLP,SSCP,BODY-WEIGHT REGULATION,OBESE GENE-PRODUCT,LEPTIN PATHWAYS,OB/OB MICE,RADIATION},
  language     = {eng},
  number       = {4},
  pages        = {189--192},
  title        = {Bovine melanocortin receptor 4: cDNA sequence, polymorphisms and mapping},
  url          = {http://dx.doi.org/10.1046/j.1365-2052.2001.00750.x},
  volume       = {32},
  year         = {2001},
}

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